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Jatropha curcas alkane type diterpenoid compound, preparation method and application thereof

A technology of jatrophane and diterpenoids, which is applied in the field of medicine, can solve the problems of less chemical components and pharmacological effects, and achieve the effects of easy industrial scale-up production, simple preparation method, and environmental friendliness

Pending Publication Date: 2021-09-03
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are relatively few studies on the chemical components and pharmacological effects of lacquer, and there is still an urgent need for further research and development of functional active ingredients in lacquer to better develop its medicinal value.

Method used

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  • Jatropha curcas alkane type diterpenoid compound, preparation method and application thereof
  • Jatropha curcas alkane type diterpenoid compound, preparation method and application thereof
  • Jatropha curcas alkane type diterpenoid compound, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A kind of preparation method of jatropha alkane type diterpenoid mainly comprises the following steps:

[0032] (1) 900 g of lacquer was heated and refluxed with 75% ethanol to extract 3 times (each time was 7.5 L, each extraction was 2 hours), and after merging, the extract was recovered under reduced pressure to obtain a crude extract;

[0033] (2) Dissolve the crude extract obtained in step (1) in water (crude extract and water weight volume ratio=1:3, use petroleum ether to extract, according to the volume ratio of the aqueous phase and the organic phase 1:1, extract 5 times , to obtain the extract;

[0034] (3) The petroleum ether extract in step (2) is separated by silica gel column chromatography, and eluted successively with a mixed solvent of petroleum ether and ethyl acetate with a volume ratio of 100:3, 100:8, and 100:10;

[0035] (4) The sherwood oil obtained in the above-mentioned step (3): the fraction of ethyl acetate volume ratio 100:8~100:10 is through...

Embodiment 2

[0050] A kind of preparation method of jatropha alkane type diterpenoid mainly comprises the following steps:

[0051] (1) 1000 g of lacquer was heated and refluxed with 80% ethanol to extract twice (each dosage: 10 L, each extraction 4h), and the extract was recovered under reduced pressure to obtain a crude extract;

[0052] (2) The ethanol extract obtained in step (1) is dissolved in water (crude extract and water weight volume=1:4), extracted with petroleum ether, and extracted 3 times according to the volume ratio of the aqueous phase and the organic phase of 1:1, get the extract;

[0053] (3) The petroleum ether extract in step (2) is separated by silica gel column chromatography, and eluted successively with a mixed solvent of petroleum ether and ethyl acetate with a volume ratio of 100:2, 100:4, 100:8, and 100:10;

[0054] (4) Petroleum ether obtained in the above step (3): Ethyl acetate volume ratio 100:8~100:10 Fraction is subjected to ODS chromatography, with volum...

Embodiment 3

[0058] A kind of preparation method of jatropha alkane type diterpenoid mainly comprises the following steps:

[0059] (1) 500 g of lacquer was heated and refluxed with 85% ethanol to extract 3 times (each dosage: 5 L, each extraction 6 h), and the extract was recovered under reduced pressure to obtain a crude extract;

[0060] (2) The ethanol extract obtained in step (1) is dissolved in water (crude extract and water weight volume=1:3), extracted with petroleum ether, the volume ratio of the aqueous phase and the organic phase is 1:1, and extracted 4 times to obtain Extracts;

[0061] (3) Petroleum ether extract in step (2), separated by silica gel column chromatography, washed with a mixed solvent of dichloromethane and acetone in volume ratios of 100:2, 100:4, 100:6, 100:8, 100:10 off;

[0062](4) Dichloromethane obtained in the above-mentioned step (3): Acetone volume ratio 100:6~100:8 fraction is through ODS chromatography, with volume ratio 30:70, 50:50, 60:40, 70:30, ...

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Abstract

The invention discloses a jatropha curcas alkane type diterpenoid compound, a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: firstly extracting euphorbia helioscopia by using an ethanol aqueous solution to obtain a crude extract, and then sequentially carrying out extraction, ODS chromatographic column and HPLC chromatographic multi-stage separation to obtain the jatropha curcas alkane type diterpenoid compound. According to the invention, the preparation method of the jatropha curcas alkane type diterpenoid compound is simple, easy to operate, convenient for industrial large-scale production, and suitable for industrial large-scale production of the jatropha curcas alkane type diterpenoid compound; and the jatropha curcas alkane type diterpenoid compound prepared by the invention has obvious cytotoxicity to a drug-resistant cell line A549 / Taxol, and is expected to be used as a potential drug for treating drug-resistant lung cancer.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to jatrophane diterpenoids in lacquer and its preparation method and application. Background technique [0002] Zeqi, also known as Wuduoyun, Wudengcao, Wufengcao, etc., is a plant of Euphorbia helioscopia L. in the family Euphorbiaceae (Euphorbiaceae). The whole plant is used as medicine, and it is distributed in the whole country except Xinjiang and Tibet. everywhere. Zeqi has always been used as a folk herbal medicine. It tastes bitter, slightly cold in nature, poisonous, and returns to the lung, small intestine, and large intestine meridian. According to the records of "Compendium of Materia Medica", it has the effects of diuresis, swelling, phlegm and fever, stagnation and insecticide. It is clinically used to treat ascites, edema, tuberculosis, phlegm, cough and asthma, and it is also used to treat cervical cancer, esophageal cancer, etc., and has certain curati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/78C07C67/48C07C67/56C07C67/58A61K31/235A61P35/00
CPCC07C67/48C07C67/56C07C67/58A61P35/00C07C2602/32C07B2200/07C07C69/78
Inventor 李宁周地陈刚李巍
Owner SHENYANG PHARMA UNIVERSITY
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