45 results about "Phorbol ester" patented technology

The invention relates to a method for removing phorbol esters from Jatropha curcas seed oil by extraction. The method comprises drying Jatropha curcas seed oil meal obtained from pre-squeezing and leaching processes, grinding into powder, mixing with industrial methanol at a weight ratio of 3.5:1.1 at 30 DEG C for more than 20 min, filtering to separate oil meal and methanol phase, collecting the oil meal and heating to remove residual methanol in the oil meal to obtain detoxicated plant protein; and concentrating the separated methanol phase to obtain phorbol ester product. After removing phorbol esters by methanol extraction, the content of phorbol esters in the oil meal is reduced from 1.78% to 0.014%, so that the oil meal can be used in preparing feed proteins.

The present invention provides an inhibitor of NF-κB, guggulsterone and its analogs. Guggulsterone suppresses NF-κB activation induced by TNF, phorbol ester, okadaic acid, cigarette smoke, H2O2 and IL-1β, as well as constitutive NF-κB activation expressed in most tumor cells. One mechanism by which guggulsterone inhibits activation of NF-κB is through suppression of IκBα phosphorylation and IκBα degradation. NF-κB-dependent gene transcription is modulated by guggulsterone and its analogs. In particular, induction by TNF, TNFR1, TRADD, TRAF2, NIK and IKK, is modulated by guggulsterone and its analogs. In addition, guggulsterone decreased the expression of genes involved in anti-apoptosis (IAP1, XIAP, Bfl-1 / A1, bcl-2, cFLIP, survivin), proliferation (cyclin D1, c-myc) and metastasis (MMP-9, COX2 and VEGF).

Methods and compositions containing a phorbol ester or a derivative of a phorbol ester are provided for the treatment of cytopathic diseases. Cytopathic diseases may be caused by a variety means suchas viral infections like HIV and AIDS, or the development of neoplasms in a mammalian subject. The methods and compositions of the invention are effective for inhibiting de novo HIV infection, upregulating viral expression from latent provirus, inhibiting HIV-induced cytopathic effects, down regulating the HIV receptor, increasing ThI cytokine expression, decreasing Th2 cytokine expression, increasing ERK phosphorylation, inducing apoptosis in malignant cells, inducing remission, maintaining remission, as chemotherapeutic agents, as well as for decreasing symptoms of cytopathic diseases and opportunistic infections that may accompany such diseasesl. Additional compositions and methods are provided which employ a phorbol ester or derivative compound in combination with at least one additional agent such as those used in HAART protocols, therapeutic agents used to treat opportunistic infections due to HIV, or chemotherapeutic agents to yield more effective treatment tools against cytopathic diseases in mammalian subjects.

The invention belongs to the technical fields of molecular biology and genetic engineering, and provides an encoding sequence for encoding cDNA of Jatropha curcas farnesyl pyrophosphate synthase. The farnesyl pyrophosphate synthase catalyzes isopentenyl pyrophosphate and dimethylallyl pyrophosphate to carry out continuous condensation reaction to form farnesyl pyrophosphate, is key enzyme for metabolic pathway of Jatropha curcas isoprene, and contributes to reducing the content of phorbol esters and precursor thereof in the Jatropha curcas. Due to the use of the Jatropha curcas protein, substances interacting with the Jatropha curcas protein, or receptors, inhibitors or antagonists, and the like can be screened by various conventional screening methods. The encoding sequence for encoding the cDNA of the Jatropha curcas farnesyl pyrophosphate synthase has great application prospects.

The invention discloses a method for detoxifying de-oiled jatropha curcas kernels. The method comprises the following steps of: crushing the de-oiled jatropha curcas kernels obtained by cold pressing into granules with granule diameter of less than 40 meshes, and performing dry steaming on the granules for 20 to 30 minutes at the temperature of between 115 and 121 DEG C under the pressure of between 0.05 and 0.1MPa; adding industrial ethanol into the granules in a weight / volume ratio of 1:3-1:5, performing ultrasonic oscillation for 25 to 30 minutes in ultrasonic wave, repeating the ultrasonic oscillation once, filtering, and separating oil meal and ethanol phase to obtain detoxified jatropha curcas kernels and ethanol-soluble crude phorbol ester product. The method has remarkable detoxifying effect, the process is simple and convenient, the extraction period is short, and the method is energy-saving and environmentally-friendly; the used ethanol is convenient and easily obtained and can be reclaimed to reduce the cost; and the method does not produce toxicity, is safe, is a renewal method for detoxifying the jatropha curcas kernels, and can be greatly popularized and applied.

Described are methods for extracting oil and phorbol esters from oil seed kernel, for example from Jatropha curcas oil seed kernel. The methods comprise treating the oil seed kernel with at least one solvent and separating the resultant solvent / oil mix from the treated kernel to leave a seedcake substantially free of phorbol esters. Also described are seedcakes produced by the methods which can be used as nutritional compositions, for example as animal feeds.

The invention provides a novel purpose of triptolide and a triptolide derivative. The triptolide can obviously inhibit the GFP fluorescent protein and P24 antigen rise effect in a phorbol ester activated lymphocyte model. When the concentration of the lymphocyte concentration is higher, the GFP positive cell percentage and the P24 antigen concentration are lower; the negative dosage-effect relationship exists. Even when the concentration of the methylprednisolone is as high as 400 uM, the inhibition effect cannot reach the inhibition effect of the triptolide with the concentration being 0.02 uM; the cell apoptosis proportion obviously exceeds the triptolide with the concentration being 0.02 uM. According to the principle, the triptolide inhibits the G0 / G1 period cell proportion rise and S period cell proportion descending due to PMA; the cell period is promoted to be stopped in the unactivated state; the triptolide achieves the effect of inhibiting the lymphocyte cell proliferation and activation through regulating the cell period; meanwhile, the effect of inhibiting the virus replication is also achieved. The triptolide and the triptolide derivative can replace glucocorticoid analogues or can be combined with the glucocorticoid analogues to be used, and are used for treating and / or preventing lung injury diseases.

The invention discloses a method for extraction and separation purification of phorbol ester components of jatropha curcas seeds and a use of the phorbol ester components. Phorbol ester components of jatropha curcas are subjected to ethyl acetate extraction and column chromatography so that total phorbol ester components are obtained. The total phorbol ester components are treated by a semipreparative high-performance liquid chromatography so that five phorbol ester monomer components are obtained. The method provided by the invention provides a foundation for further exploitation and utilization of phorbol ester components of jatropha curcas.

The present invention relates to a method for detoxifying plant constituents from Jatropha, a detoxified material of Jatropha for producing a nourishment and uses thereof for producing a nourishment. Detoxification is performed with alkali (sodium hydroxyde) and short chain alcohol (methanol), resulting in removal of toxic phorbol esters.

The invention provides a jatropha curcas seed oil detoxification method, and belongs to the field of fine and deep processing of jatropha curcas seeds and plant oil production, wherein ultraviolet light irradiation is adopted to degrade phorbol ester, and then ethanol with an appropriate concentration is adopted to elute, such that toxin residue in jatropha curcas seed oil is solved. The method has the following characteristics that: equipment requirements are not high, the production process is simple, the production process conditions are mild, operations are convenient, industrialization implementation can be achieved, strong acid and strong alkali treatments are not required, an environmentally friendly property is provided, the used raw material ethanol is a commonly-used reagent in the field of deep processing of vegetable oils, and is suitable for large-scale use, and more importantly important significance is provided for releases of edible oil strategy safety crisis in China.

The invention relates to a preparation method of a phorbol ester compound Euphorbia Factor L2. A two-dimensional liquid chromatographic technology is adopted to extract Euphorbia Factor L2 from leptochloa chinensis, through reasonable selection of a chromatographic column, a dissolved solution, a mobile phase and the like, optimization of technical conditions such as the flow velocity, the elution mode, the collection section and the like and the orthogonality of the first dimension and the second dimension, complementary separation is realized, so that the whole separation and purification method is practicable, the operability is high, and the purity of the obtained Euphorbia Factor L2 is higher than 98% at most.

The invention discloses a method for improving the detection rate of cytokines secreted by peripheral blood leukocytes. The method comprises the following steps: S1, taking whole blood and adding a culture solution to dilute the whole blood; S2, adding phorbol ester and ionomycin calcium salt, carrying out culturing for 6 hours, and adding monensin in the later 4 hours of the culture so as to obtain cells to be detected; S3, adding a cell surface labeled antibody into the cells to be detected for staining of the cells to be detected, dissolving red blood cells with a whole blood sample, carrying out washing with a staining buffer, adding polyformaldehyde for lucifugal immobilization, adding a membrane permeating buffer for membrane permeation, adding bovine serum albumin and mouse serum for blocking, adding a cytokine antibody for staining, carrying out washing with membrane permeating buffer, and carrying out washing with a staining buffer to obtain stained cells; and S4, carrying outdetection with a flow cytometer. The method of the invention has a wide application range and high ratio of cytokine secreting cells, and experimental data and control data have statistical significance.

The invention discloses application of Klebsiella variicola in fermentation detoxification of a Jatropha curcas seed cake and a fermentation detoxification method of the Jatropha curcas seed cake. The method comprises the following steps: (1) taking Klebsiella variicola LY-1 and activating Klebsiella variicola LY-1 so as to obtain a seed liquid; (2) taking the Jatropha curcas seed cake, adding water, carrying out uniform mixing and inoculating the seed liquid prepared in the step (1), wherein the inoculation amount of the seed liquid is 20 to 100% (v / w) of the Jatropha curcas seed cake, and the initial addition amount of water is 50 to 150% (v / w) of the Jatropha curcas seed cake; and (3) carrying out fermentation at 27 to 47 DEG C for 24 to 72 h. The method improves the degradation rate of phorbol ester in the Jatropha curcas seed cake, shortens fermentation time, does not destroy nutritional components in the seed cake, is simple and convenient and has low cost and good market application prospects.

The invention discloses a method for detoxifying de-oiled jatropha curcas kernels. The method comprises the following steps of: crushing the de-oiled jatropha curcas kernels obtained by cold pressing into granules with granule diameter of less than 40 meshes, and performing dry steaming on the granules for 20 to 30 minutes at the temperature of between 115 and 121 DEG C under the pressure of between 0.05 and 0.1MPa; adding industrial ethanol into the granules in a weight / volume ratio of 1:3-1:5, performing ultrasonic oscillation for 25 to 30 minutes in ultrasonic wave, repeating the ultrasonic oscillation once, filtering, and separating oil meal and ethanol phase to obtain detoxified jatropha curcas kernels and ethanol-soluble crude phorbol ester product. The method has remarkable detoxifying effect, the process is simple and convenient, the extraction period is short, and the method is energy-saving and environmentally-friendly; the used ethanol is convenient and easily obtained and can be reclaimed to reduce the cost; and the method does not produce toxicity, is safe, is a renewal method for detoxifying the jatropha curcas kernels, and can be greatly popularized and applied.

Methods and compositions containing a phorbol ester or a derivative of a phorbol ester are provided for the treatment of chronic and acute conditions. Such conditions may be caused by disease, be symptoms or sequelae of disease. Chronic and acute conditions may be due to viral infections such as HIV and AIDS, neoplastic diseases stroke, kidney disease, urinary incontinence, autoimmune disorders, Parkinson's disease, prostate hypertrophy, aging, or the treatment of such diseases. Additional compositions and methods are provided which employ a phorbol ester or derivative compound in combination with at least one additional agent to yield more effective treatment tools against acute and chronic conditions in mammalian subjects.

The present invention relates to phorbol esters from the seeds of Aquilaria malaccensis by a series of chromatographic processes, and compositions containing these congeners for the treatment or prevention of allergic responses. Furthermore, compositions are provided which comprise these phorbol esters in combination with at least one to assess the effects for preventing or treating allergies.

The invention relates to a functional strain, and relates to a strain for highly effectively degrading phorbol ester and application of the strain in fermenting detoxifying jatropha curcas cake meal.The strain for highly effectively degrading the phorbol ester is a strain Z11 of enterobacter cloacae; the strain is preserved in China General Microbiological Culture Collection Center on September 21st, 2017 (address: Institute of Microbiology, Chinese Academy of Sciences, 3#, Yard 1, West Beichen Road, Chaoyang District, Beijing, postal code:100101), and the number is CGMCC No.14655. The straincan effectively reduce toxic components and anti-nutrient factor content in the jatropha curcas cake meal, and can improve the feeding value of the jatropha curcas cake meal.

The invention discloses a barbadosnut phorbol ester water emulsion insecticide. The barbadosnut phorbol ester water emulsion insecticide comprises the following components by weight: 1-20 percent of barbadosnut phorbol ester, 5-20 percent of a solvent, 3-20 percent of an emulsifier, 5-20 percent of a cosolvent, 2-5 percent of an antifreeze, 2-15 percent of a stabilizer, and the balance of water. The invention further discloses a preparation method of the barbadosnut phorbol ester water emulsion insecticide. Due to the fact that effective component of the product provided by the invention is phorbol ester extracted from barbadosnut seed oil and obtained as one of the side products generated producing a biodiesel, not only the barbadosnut resource is used comprehensively and the gap of using phorbol ester as the environment-friendly botanic pesticides is filled, but also the source of raw materials is wide, the cost is low, and the insecticide is easy to degrade and has no pollution to the environment. At the same time, as phorbol ester is a secondary metabolite of the plants, the product is featured with a wide insecticidal spectrum and various use ways, and is convenient to popularize.

The invention discloses a composite acid pickling corrosion inhibitor of barbadosnut cake meal extract and a preparation method of the composite acid pickling corrosion inhibitor, and belongs to the technical field of corrosion inhibitors. The process of extracting an acid corrosion inhibitor from barbadosnut cake meal is optimized, toxalbumin and phorbol ester are removed, the problem that the acid corrosion inhibitor extracted from the barbadosnut cake meal is toxic is solved, shaddock peel extract and the barbadosnut cake meal extract suffer from synergistic interaction, the corrosion inhibition efficiency is high, high adaptability is achieved, the effect of corrosion of acid on steel and aluminum is obviously inhibited, and more importantly, a good corrosion inhibition effect is achieved for zinc and zinc and aluminum alloy. The corrosion inhibitor is safe, free of harm, biodegradable and convenient to use, can be applied to washing petrochemical equipment, boilers and even tap water pipelines, and has good development prospects.

The invention provides application of an extract composition of natural variform arsenite. The extract composition comprises extract of natural variform arsenite and at least one of the following anti-tumor medicines: camptothecin, vincaleukoblastinum, vincristine, taxol, tumor necrosis factor, interleukin, interferon, transforming growth factor-beta, lipopolysaccharide, phorbol ester, adenylate cyclase activating agent andbone morphogenetic protein 4, wherein the weight of the extract of the natural variform arsenite accounts for 0.001-99.9 percent of the total weight of the prepared medicine. The extract composition of natural variform arsenite can be used for preparing anti-tumor stem cells, anti-hepatitis B and anti-AIDS (acquired immune deficiency syndrome) virus medicines, can be used for inhibiting hepatitis B and resisting AIDS and other viruses, and expands the application range to development and utilization of natural arsenite preparations.

The invention relates to a preparation method of a phorbol ester compound Euphorbia Factor L1. According to the method, a QJZ-6 original extract is all dissolved and separated with a preparation liquid phase normal-phase DAC (dynamic axial compression) chromatographic column, a binary mobile phase system is adopted for elution separation, a mobile phase A and a mobile phase B adopt combination of normal hexane and ethanol, the elution mode adopts gradient elution, the detection wavelength is 210 nm, and a target peak is collected and dried to obtain a target monomeric compound. The method has simple steps, good repeatability and high practicality and meets requirements for large-scale industrial production, and the purity of the separated and purified phorbol ester compound Euphorbia Factor L1 can be as high as 99%.

The invention belongs to the field of detection, and particularly relates to a kit for rapidly detecting active tuberculosis. The kit comprises a stimulating protein and a stimulant, a mixed stimuluscomposed of the stimulating protein and the stimulant is used as a specific stimulating antigen to stimulate peripheral blood lymphocytes to produce cell factors IFN- gamma and IL-2, and the purpose of judging whether the disease is the active tuberculosis is achieved by detecting the secretion concentration of IFN- gamma and IL-2. The stimulating protein of the kit provided by the invention comprises CFP-10, ESAT-6 and Rv 1985c, and the stimulant comprises a phorbol ester polyclonal stimulant and ionomycin. The stimulating protein can stimulate tuberculosis-specific effector T cells in the lymphocytes to secrete the cell factors, and the stimulant can stimulate the activated lymphocytes to enhance the ability of synthesizing and secreting the cell factors. By adopting the kit provided bythe invention, the specificity of the detection result can be greatly improved, and whether the disease is the active tuberculosis can be judged more accurately.

The invention relates to 3-hydroxyl-3-mathylglutaryl-CoA reductase albumen encoding sequence of barbadosnut, which belongs to the technical field of molecular biology and gene engineering. The DNA molecule separation comprises the following steps: the nucleotide sequence of polypeptide with barbadosnutJcHMGRs albumen activity is encoded, and the nucleotide sequence has a homology of at least 70 percent with the nucleotide sequence with nucleotide ranging from bit 81-1832 in SEQ ID NO.1; the nucleotide sequence can be hybridized with the nucleotide sequence with nucleotide ranging from bit 81-1832 in SEQ ID NO.2 at 40-55 DEG C. The invention is an enzyme, namely, 3-hydroxyl-3-mathylglutaryl-CoA generated by catalyzing acetyl-CoA and acetoacetyl coenzyme A. The enzyme is favorable for reducing the content of phorbol ester or the prosoma thereof in barbadosnut and greatly helpful for cultivating quality variety of aseptic barbadosnut.

The invention relates to a method for obtaining detoxified jatropha curcas oil from seeds of jatropha curcas which is an energy plant, and belongs to the fields of comprehensive utilization of energyplants and production of vegetable oil. According to the method, detoxified jatropha curcas oil is extracted from seeds of jatropha curcas via a simple two-step method by adoption of two solvents, andcake with low toxicity and a phorbol-ester-enriched extract with high toxicity are obtained at the same time. The method is simple to operate, high in product yield, low in cost, and suitable for massive production. The method has a high value with respect to deep processing and added value increase of jatropha curcas resource.