Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Klebsiella xenophobia in the detoxification of Jatropha curcas cake fermentation

A technology of Klebsiella and Jatropha meal, which is applied in the field of fermentation to achieve the effects of improving the degradation rate, shortening the fermentation time and the method being simple and convenient

Active Publication Date: 2016-05-04
SICHUAN UNIV
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on its application in the detoxification of Jatropha curcas cake

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Klebsiella xenophobia in the detoxification of Jatropha curcas cake fermentation
  • Application of Klebsiella xenophobia in the detoxification of Jatropha curcas cake fermentation
  • Application of Klebsiella xenophobia in the detoxification of Jatropha curcas cake fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 adopts the inventive method to Jatropha curcas cake fermentation detoxification

[0041] 1.1 Medium

[0042] 1) LB liquid medium: sterilized by moist heat at 121°C for 20 minutes, and set aside.

[0043] 2) Solid-state fermentation medium: Jatropha curcas cake powder 5g, water, natural pH, non-sterilized.

[0044] 1.2 Preparation of bacterial suspension

[0045] After activating Klebsiella xenophila LY-1 with LB solid medium, pick and inoculate them into LB liquid medium, and place them in a constant temperature shaker at 37°C and 180r / min to culture the bacterial suspension. The time is 14h, making the strain concentration reach 10 8 cfu / mL to obtain the seed solution of strain LY-1.

[0046] 1.3 Solid state fermentation test

[0047] The cake of Jatropha curcas in Liren Township, Yongsheng County, Yunnan Province was used as raw material (the initial content of phorbol ester was 2.32mg / g).

[0048] Put the unsterilized solid-state fermentation mediu...

Embodiment 2

[0054] Embodiment 2 adopts the inventive method to Jatropha curcas cake fermentation detoxification

[0055] 1.1 Medium

[0056] 1) LB liquid medium: sterilized by moist heat at 121°C for 20 minutes, and set aside.

[0057] 2) Solid-state fermentation medium: Jatropha curcas cake powder 5g, water, natural pH, non-sterilized.

[0058] 1.2 Preparation of bacterial suspension

[0059] After activating Klebsiella xenophila LY-1 with LB solid medium, pick and inoculate them into LB liquid medium, and place them in a constant temperature shaker at 37°C and 180r / min to culture the bacterial suspension. The time is 14h, making the strain concentration reach 10 8 cfu / mL to obtain the seed solution of strain LY-1.

[0060] 1.3 Solid state fermentation test

[0061] The cake of Jatropha curcas in Liren Township, Yongsheng County, Yunnan Province was used as raw material (the initial content of phorbol ester was 2.32mg / g).

[0062] Put the unsterilized solid-state fermentation mediu...

Embodiment 3

[0068] Embodiment 3 adopts the inventive method to Jatropha curcas cake fermentation detoxification

[0069] 1.1 Medium

[0070] 1) LB liquid medium: sterilized by moist heat at 121°C for 20 minutes, and set aside.

[0071] 2) Solid-state fermentation medium: Jatropha curcas cake powder 5g, water, natural pH, non-sterilized.

[0072] 1.2 Preparation of bacterial suspension

[0073] After activating Klebsiella xenophila LY-1 with LB solid medium, pick and inoculate them into LB liquid medium, and place them in a constant temperature shaker at 37°C and 180r / min to culture the bacterial suspension. The time is 14h, making the strain concentration reach 10 8 cfu / mL to obtain the seed solution of strain LY-1.

[0074] 1.3 Solid state fermentation test

[0075] The cake of Jatropha curcas in Liren Township, Yongsheng County, Yunnan Province was used as raw material (the initial content of phorbol ester was 2.32mg / g).

[0076] Put the unsterilized solid-state fermentation mediu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of Klebsiella variicola in fermentation detoxification of a Jatropha curcas seed cake and a fermentation detoxification method of the Jatropha curcas seed cake. The method comprises the following steps: (1) taking Klebsiella variicola LY-1 and activating Klebsiella variicola LY-1 so as to obtain a seed liquid; (2) taking the Jatropha curcas seed cake, adding water, carrying out uniform mixing and inoculating the seed liquid prepared in the step (1), wherein the inoculation amount of the seed liquid is 20 to 100% (v / w) of the Jatropha curcas seed cake, and the initial addition amount of water is 50 to 150% (v / w) of the Jatropha curcas seed cake; and (3) carrying out fermentation at 27 to 47 DEG C for 24 to 72 h. The method improves the degradation rate of phorbol ester in the Jatropha curcas seed cake, shortens fermentation time, does not destroy nutritional components in the seed cake, is simple and convenient and has low cost and good market application prospects.

Description

technical field [0001] The invention relates to the application of Klebsiella variicola in the fermentation and detoxification of Jatropha curcas cake, which belongs to the field of fermentation. Background technique [0002] Jatropha curcas (Jatrophacurcas L.) is recognized worldwide as the most likely tree species with great development potential to replace fossil energy in the future, and the biodiesel produced by processing Jatropha curcas oil is close to or even exceeds the national "0" in various performance indicators No. diesel oil and national biodiesel standards, so the production of biodiesel from Jatropha curcas fruit has become an active direction of research and production of biodiesel now. Jatropha curcas seed cake (JCSC) is a by-product of the biodiesel production process, which has high protein content and high nutritional value, but because of its high toxicity, it is mainly used as fertilizer or directly discharged into the natural environment It not only...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12C12N1/20C12R1/22A23K10/37
CPCY02P60/87
Inventor 唐琳刘莹陈放
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com