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Method for extraction and separation purification of phorbol ester components of jatropha curcas seeds

A technology for separating and purifying Jatropha curcas, which is applied in the field of resource botany and can solve the problems of complex extraction process route, unsuitable for rapid separation and preparation of phorbol ester components, phorbol ester monomer components, etc.

Inactive Publication Date: 2012-09-12
SICHUAN UNIV
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Problems solved by technology

[0005] Especially in recent years it has been widely reported that the phorbol ester components in Jatropha curcas have significant biological activity or have the potential to induce tumorigenesis and be used as a tumor-inducing agent, so the preparation of its phorbol ester components, especially high-purity phorbol The ester component is particularly important. Although some literatures have carried out the extraction, separation and purification research of its phorbol ester components, due to its complicated extraction process route, it is not suitable for rapid separation and preparation of high-content phorbol ester components and high Purity phorbol ester monomer components

Method used

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  • Method for extraction and separation purification of phorbol ester components of jatropha curcas seeds
  • Method for extraction and separation purification of phorbol ester components of jatropha curcas seeds
  • Method for extraction and separation purification of phorbol ester components of jatropha curcas seeds

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Embodiment 1

[0035] This embodiment is the extraction and separation method of total phorbol esters in Jatropha curcas seeds.

[0036] The dried 2kg of Jatropha curcas seeds were crushed through a 200-mesh sieve, and after using 10L ethyl acetate for 24 hours, the cold soaking liquid was filtered, and then 24 hours of 10L ethyl acetate was used to combine the two extracts, and the extracts were placed in a large centrifuge Centrifuge at a speed of 4000r / min for 20 minutes, combine the supernatant, take the supernatant:water ratio of 1:1 for extraction, combine the ethyl acetate phase, and discard the water phase to remove the polarity in the supernatant For stronger substances, concentrate the ethyl acetate phase in a rotary evaporator, recover the ethyl acetate for reuse, and the remaining oily liquid in the flask, about 800mL, transfer it to a volumetric flask, store it in the refrigerator, and take the silica gel Dissolve 3L in about 500mL of petroleum ether, mix well and degas, then pu...

Embodiment 2

[0039] This embodiment is a method for separating and purifying phorbol ester monomer components in Jatropha curcas seeds.

[0040] The total phorbol ester components showing the characteristic peaks of phorbol esters in the extraction example were dissolved in acetonitrile-water (8:2), and passed through a filter membrane with a diameter of 0.45 μm for preparation and analysis. The preparative separation chromatographic conditions are: chromatographic column, C8 column (Welchrom TM, 5μm, 10.0×250mm); mobile phase, acetonitrile-water (80:20); detection wavelength, 280nm and 254nm. The total phorbol esters were separated and purified by Shimadzu LC-6AD semi-preparative liquid chromatography, and compounds 1-5 were collected at 37.93, 45.41, 52.36, 57.99, and 63.77 min, respectively. The purity of compounds 1-5 was tested by HPLC-UV method, and the impure compounds were re-prepared. Compounds 1~5 were identified by LC / micrOTOF-Q (Agilent 1260, Bruker-micrOTOF-QII) while referr...

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Abstract

The invention discloses a method for extraction and separation purification of phorbol ester components of jatropha curcas seeds and a use of the phorbol ester components. Phorbol ester components of jatropha curcas are subjected to ethyl acetate extraction and column chromatography so that total phorbol ester components are obtained. The total phorbol ester components are treated by a semipreparative high-performance liquid chromatography so that five phorbol ester monomer components are obtained. The method provided by the invention provides a foundation for further exploitation and utilization of phorbol ester components of jatropha curcas.

Description

technical field [0001] The invention belongs to the field of resource botany, in particular to a method for extracting, separating and purifying phorbol ester components in Euphorbiaceae resource plants, especially the extraction, separation and purification method for phorbol ester components in Euphorbiaceae resource plant Jatropha curcas seeds method. Background technique [0002] Jatropha curcas is a plant of the Euphorbiaceae Jatropha genus. It is a poisonous traditional medicinal resource plant in most countries and regions of Africa, America, and Asia. It is widely used in the treatment of various diseases. The whole plant is poisonous, and the seeds are the most poisonous. , followed by leaves. Jatropha curcas is a blindly poisonous traditional Chinese medicine in the system of traditional Chinese medicine. It is recorded in "Dictionary of Traditional Chinese Medicine" and other works, and it is often used in clinical practice after processing or compounding toxins....

Claims

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Application Information

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IPC IPC(8): C07C69/33C07C67/56
Inventor 陈放唐琳王战国颜钫魏磊张伟
Owner SICHUAN UNIV
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