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Application of extract composition of natural variform arsenite

A technology for extracting liquid and composition, which is applied in the field of extracting liquid composition of natural various forms of arsenic, can solve the problems of inability to meet, no HIV virus, inability to form complexes with other drugs or excipients, etc.

Active Publication Date: 2014-05-07
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the preparation technology reported in modern times includes methods such as water flying + vinegar production, nano- and micronization, and the result is only a suspension, which cannot meet the requirements of current pharmaceutical preparations. A acidophilic Thiobacillus for metallurgy, extracting realgar to produce a mixed extract containing arsenic acid (III, V), methyl MMA (V) and a large amount of auxiliary elements (iron and iron compounds) (public documents are as follows: Li Hongyu, Zhang Jinghong, Zhi Dejuan. Heavy metal removal method in mineral medicine. Chinese patent [P]: 200610200067.5, 2006-1-02.), although these methods can obtain a mixed solution containing some forms of arsenic, but due to the mixed Other ingredients, whose exact active ingredients cannot be determined at present, cannot form complexes with other drugs or excipients, and its in vitro inhibitory effect on tumor cells such as lung cancer and liver cancer is significantly lower than that of As 2 o 3 , and more importantly, it has not yet been seen that it can inhibit tumor stem cells, hepatitis B and HIV at the same time

Method used

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  • Application of extract composition of natural variform arsenite
  • Application of extract composition of natural variform arsenite
  • Application of extract composition of natural variform arsenite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Preparation of natural extracts of various forms of arsenic

[0020] According to the invention patent application number 201010580193.4 and the publication date of 2011-04-20, a preparation method for extracting natural, polymorphic and tumor-inhibiting arsenic-containing compounds from marine microorganisms and the preparation method described in the application Realgarite (As 2 o 3 , content 36%, As 4 S 4 , content 62%) and or orpiment (As 2 S 3 , 72%, AsC, 25%) ore powder, the implementation plan specifically includes the following steps:

[0021] Step 1: After crushing 20g of arsenic ore powder to 200 mesh, add a mixed solution consisting of 1mol / L sulfuric acid and 15% ferric chloride (volume ratio 8 :2), and then cultivated in a constant temperature shaker with a rotation speed of 180r / min and a temperature of 30°C for 15 days to obtain an extract; filter the extract with a filter membrane with a pore size of 0.45 μm to obtain a filtrate; Under ...

Embodiment 2

[0027] Embodiment 2: Preparation of the extract composition of natural various forms of arsenic

[0028] Drugs taken include: camptothecin (3umol / L), vinblastine (VLB, 0.2ug / mL), vincristine (VCR, 0.16ug / mL), paclitaxel (Taxol, 1umol / L), tumor necrosis factor (TNF, 25ng / ml), interleukin (interleukin, IL-6, 10ng / ml), interferon (IFN, 10ng / ml), transforming growth factor-β (TGF-β, 2.5ng / ml), lipid Polysaccharide (LPS, 1ug / mL), phorbol ester (TPA, 20ng / ml), adenylyl cyclase activator (Forskolin, 20ng / ml) and bone morphogenetic protein 4 (BMP-4, 10ng / ml ) and other drug solutions, add 0.05mol / L Tris-HCL buffer solution (Tris-HCL) to 1mL, boil in 80°C water bath for 1 hour, centrifuge and filter according to the drug Extraction: the extract of natural various forms of arsenic (1:2, volume ratio), add the extract A or B or C of natural various forms of arsenic prepared in Example 1, add 50ul of lyase to the filtrate (concentration is 0.0167u / mL), 37°C, enzymatic reaction for 2 hou...

Embodiment 3

[0032] Example 3. Experimental example of pharmacodynamic evaluation of the extract composition of natural polymorphic arsenic

[0033] 1. In the following experimental examples, the test sample: the composition of the extract solution containing natural various forms of arsenic in the extract solution B:

[0034] Composition 1: The composition of the arsenic compound content in each unit preparation is determined as follows: arsenic acid (V, 1.5nM); arsenous acid (III, 0.7nM); methyl arsenic acid (III, 0.3nM), methyl Arsenic acid (V, 2.5nM); Monomethyl-thioarsenic acid (0.7nM); Dimethyldithioarsenic acid DMDTA (0.8nM); Dimethylarsenic acid (DMA, 0.6M); Arsine oxygen (TMAO, 1.5nM); arsenoborane (AsB, 0.4nM); arsenocholine (AsC, 1nM); the content and composition of other drugs in each unit preparation were detected as follows: Tumor necrosis factor (TNF, 25ng / ml), the combination ratio of other drugs and arsenic compounds is 1:1;

[0035]Composition 2: The composition of the...

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Abstract

The invention provides application of an extract composition of natural variform arsenite. The extract composition comprises extract of natural variform arsenite and at least one of the following anti-tumor medicines: camptothecin, vincaleukoblastinum, vincristine, taxol, tumor necrosis factor, interleukin, interferon, transforming growth factor-beta, lipopolysaccharide, phorbol ester, adenylate cyclase activating agent andbone morphogenetic protein 4, wherein the weight of the extract of the natural variform arsenite accounts for 0.001-99.9 percent of the total weight of the prepared medicine. The extract composition of natural variform arsenite can be used for preparing anti-tumor stem cells, anti-hepatitis B and anti-AIDS (acquired immune deficiency syndrome) virus medicines, can be used for inhibiting hepatitis B and resisting AIDS and other viruses, and expands the application range to development and utilization of natural arsenite preparations.

Description

【Technical field】 [0001] The invention relates to the application of an extract composition of natural arsenic in various forms. 【Background technique】 [0002] Tumor is the most common malignant tumor that threatens human health. Despite the continuous development of radiotherapy, chemotherapy and surgery, the mortality rate is still high. For example, as a common type of non-small cell lung cancer (NSCLC), the overall 5-year survival rate is still less than 15%. Based on the theory of cancer stem cells (CSC), it is believed that cancer stem cells with self-renewal and infinite proliferation capabilities are the "root" of cancer occurrence and recurrence (public document: Yang Ming. Nano-realgar on lung cancer and liver cancer cells and The apoptosis-inducing effect of tumor stem cells. [D]. Doctoral dissertation of Lanzhou University: Lanzhou. 2010, P10-15). For this reason, a drug screening strategy aimed at inhibiting cancer stem cells will be a new way of cancer treat...

Claims

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Application Information

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IPC IPC(8): A61K38/20A61K38/21A61K38/19A61K33/36A61P35/00A61P31/20A61P31/18
Inventor 张景红杨礼
Owner HUAQIAO UNIVERSITY
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