Application of Klebsiella variicola in fermentation detoxification of Jatropha curcas seed cake

A technology for Klebsiella and Jatropha meal, which is applied in the field of fermentation to achieve the effects of shortening fermentation time, simple method and low cost

Active Publication Date: 2014-10-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on its application in the detoxification of Jatropha curcas cake

Method used

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  • Application of Klebsiella variicola in fermentation detoxification of Jatropha curcas seed cake
  • Application of Klebsiella variicola in fermentation detoxification of Jatropha curcas seed cake
  • Application of Klebsiella variicola in fermentation detoxification of Jatropha curcas seed cake

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 adopts the inventive method to Jatropha curcas cake fermentation detoxification

[0041] 1.1 Medium

[0042] 1) LB liquid medium: sterilized by moist heat at 121°C for 20 minutes, and set aside.

[0043] 2) Solid-state fermentation medium: Jatropha curcas cake powder 5g, water, natural pH, non-sterilized.

[0044] 1.2 Preparation of bacterial suspension

[0045] After activating Klebsiella xenophila LY-1 with LB solid medium, pick and inoculate them into LB liquid medium, and place them in a constant temperature shaker at 37°C and 180r / min to culture the bacterial suspension. The time is 14h, making the strain concentration reach 10 8 cfu / mL to obtain the seed solution of strain LY-1.

[0046] 1.3 Solid state fermentation test

[0047] The cake of Jatropha curcas in Liren Township, Yongsheng County, Yunnan Province was used as raw material (the initial content of phorbol ester was 2.32mg / g).

[0048] Put the unsterilized solid-state fermentation mediu...

Embodiment 2

[0054] Embodiment 2 adopts the inventive method to Jatropha curcas cake fermentation detoxification

[0055] 1.1 Medium

[0056] 1) LB liquid medium: sterilized by moist heat at 121°C for 20 minutes, and set aside.

[0057] 2) Solid-state fermentation medium: Jatropha curcas cake powder 5g, water, natural pH, non-sterilized.

[0058] 1.2 Preparation of bacterial suspension

[0059] After activating Klebsiella xenophila LY-1 with LB solid medium, pick and inoculate them into LB liquid medium, and place them in a constant temperature shaker at 37°C and 180r / min to culture the bacterial suspension. The time is 14h, making the strain concentration reach 10 8 cfu / mL to obtain the seed solution of strain LY-1.

[0060] 1.3 Solid state fermentation test

[0061] The cake of Jatropha curcas in Liren Township, Yongsheng County, Yunnan Province was used as raw material (the initial content of phorbol ester was 2.32mg / g).

[0062] Put the unsterilized solid-state fermentation mediu...

Embodiment 3

[0068] Embodiment 3 adopts the inventive method to Jatropha curcas cake fermentation detoxification

[0069] 1.1 Medium

[0070] 1) LB liquid medium: sterilized by moist heat at 121°C for 20 minutes, and set aside.

[0071] 2) Solid-state fermentation medium: Jatropha curcas cake powder 5g, water, natural pH, non-sterilized.

[0072] 1.2 Preparation of bacterial suspension

[0073] After activating Klebsiella xenophila LY-1 with LB solid medium, pick and inoculate them into LB liquid medium, and place them in a constant temperature shaker at 37°C and 180r / min to culture the bacterial suspension. The time is 14h, making the strain concentration reach 10 8 cfu / mL to obtain the seed solution of strain LY-1.

[0074] 1.3 Solid state fermentation test

[0075] The cake of Jatropha curcas in Liren Township, Yongsheng County, Yunnan Province was used as raw material (the initial content of phorbol ester was 2.32mg / g).

[0076] Put the unsterilized solid-state fermentation mediu...

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Abstract

The invention discloses application of Klebsiella variicola in fermentation detoxification of a Jatropha curcas seed cake and a fermentation detoxification method of the Jatropha curcas seed cake. The method comprises the following steps: (1) taking Klebsiella variicola LY-1 and activating Klebsiella variicola LY-1 so as to obtain a seed liquid; (2) taking the Jatropha curcas seed cake, adding water, carrying out uniform mixing and inoculating the seed liquid prepared in the step (1), wherein the inoculation amount of the seed liquid is 20 to 100% (v/w) of the Jatropha curcas seed cake, and the initial addition amount of water is 50 to 150% (v/w) of the Jatropha curcas seed cake; and (3) carrying out fermentation at 27 to 47 DEG C for 24 to 72 h. The method improves the degradation rate of phorbol ester in the Jatropha curcas seed cake, shortens fermentation time, does not destroy nutritional components in the seed cake, is simple and convenient and has low cost and good market application prospects.

Description

technical field [0001] The invention relates to the application of Klebsiella variicola in the fermentation and detoxification of Jatropha curcas cake, which belongs to the field of fermentation. Background technique [0002] Jatropha curcas (Jatrophacurcas L.) is recognized worldwide as the most likely tree species with great development potential to replace fossil energy in the future. The biodiesel produced by processing Jatropha curcas oil is close to or even exceeds the national "0" in various performance indicators. "Number diesel oil and the national biodiesel standard, so the production of biodiesel from Jatropha curcas has become an active direction of research and production of biodiesel now. Jatropha curcas seed cake (JCSC) is a by-product of the biodiesel production process, which has high protein content and high nutritional value, but because of its high toxicity, it is mainly used as fertilizer or directly discharged into the In the natural environment, not o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K10/12A23K10/37
CPCY02P60/87
Inventor 唐琳刘莹陈放
Owner SICHUAN UNIV
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