Application of Klebsiella variicola in fermentation detoxification of Jatropha curcas seed cake
A technology for Klebsiella and Jatropha meal, which is applied in the field of fermentation to achieve the effects of shortening fermentation time, simple method and low cost
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Embodiment 1
[0040] Embodiment 1 adopts the inventive method to Jatropha curcas cake fermentation detoxification
[0041] 1.1 Medium
[0042] 1) LB liquid medium: sterilized by moist heat at 121°C for 20 minutes, and set aside.
[0043] 2) Solid-state fermentation medium: Jatropha curcas cake powder 5g, water, natural pH, non-sterilized.
[0044] 1.2 Preparation of bacterial suspension
[0045] After activating Klebsiella xenophila LY-1 with LB solid medium, pick and inoculate them into LB liquid medium, and place them in a constant temperature shaker at 37°C and 180r / min to culture the bacterial suspension. The time is 14h, making the strain concentration reach 10 8 cfu / mL to obtain the seed solution of strain LY-1.
[0046] 1.3 Solid state fermentation test
[0047] The cake of Jatropha curcas in Liren Township, Yongsheng County, Yunnan Province was used as raw material (the initial content of phorbol ester was 2.32mg / g).
[0048] Put the unsterilized solid-state fermentation mediu...
Embodiment 2
[0054] Embodiment 2 adopts the inventive method to Jatropha curcas cake fermentation detoxification
[0055] 1.1 Medium
[0056] 1) LB liquid medium: sterilized by moist heat at 121°C for 20 minutes, and set aside.
[0057] 2) Solid-state fermentation medium: Jatropha curcas cake powder 5g, water, natural pH, non-sterilized.
[0058] 1.2 Preparation of bacterial suspension
[0059] After activating Klebsiella xenophila LY-1 with LB solid medium, pick and inoculate them into LB liquid medium, and place them in a constant temperature shaker at 37°C and 180r / min to culture the bacterial suspension. The time is 14h, making the strain concentration reach 10 8 cfu / mL to obtain the seed solution of strain LY-1.
[0060] 1.3 Solid state fermentation test
[0061] The cake of Jatropha curcas in Liren Township, Yongsheng County, Yunnan Province was used as raw material (the initial content of phorbol ester was 2.32mg / g).
[0062] Put the unsterilized solid-state fermentation mediu...
Embodiment 3
[0068] Embodiment 3 adopts the inventive method to Jatropha curcas cake fermentation detoxification
[0069] 1.1 Medium
[0070] 1) LB liquid medium: sterilized by moist heat at 121°C for 20 minutes, and set aside.
[0071] 2) Solid-state fermentation medium: Jatropha curcas cake powder 5g, water, natural pH, non-sterilized.
[0072] 1.2 Preparation of bacterial suspension
[0073] After activating Klebsiella xenophila LY-1 with LB solid medium, pick and inoculate them into LB liquid medium, and place them in a constant temperature shaker at 37°C and 180r / min to culture the bacterial suspension. The time is 14h, making the strain concentration reach 10 8 cfu / mL to obtain the seed solution of strain LY-1.
[0074] 1.3 Solid state fermentation test
[0075] The cake of Jatropha curcas in Liren Township, Yongsheng County, Yunnan Province was used as raw material (the initial content of phorbol ester was 2.32mg / g).
[0076] Put the unsterilized solid-state fermentation mediu...
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