The invention belongs to the technical field of bioengineering and specifically discloses a method for constructing a
ribosome inactivating
protein gene virus vector and expressing active proteins intumor cells through the
ribosome inactivated
protein gene virus vector. The method comprises the following steps that first, suitable
ribosome inactivated proteins are selected, and codon optimizationfor humanized expression is conducted on mature region genes of the ribosome inactivated proteins; second, an adenovirus carrier of
wild type and optimized genes is constructed, and recombinant viruses are packaged; and third,
recombinant adenoviruses infect the
tumor cells, the
recombinant adenoviruses express proteins in the
tumor cells through detection, and the anti-tumor effect of the
recombinant adenoviruses is detected. According to the method, an alpha-charantin
gene is subjected to
codon optimization, an
expression vector system for the adenoviruses capable of infecting the
tumor cells of mammals is constructed so as to be suitable for expressing the active proteins in the tumor cells, and the defects that in the prior art, a prokaryotic
cell expression
system can only be expressed in
escherichia coli when being used, and a
plant virus vector can only be expressed in
plant cells but cannot be expressed in the tumor cells and kill tumors when being used are overcome.