Method for efficiently producing jatropha M_2 seeds processed by EMS (methanesulfonic acid ethyl ester)

A technology of Jatropha curcas and seeds, applied in the field of plant mutation and reproductive biology, can solve the problem of difficulty in mass production, insufficient mutagenesis efficiency and quality, and inability to quickly and effectively obtain high-yield, high-quality, and stress-resistant leprosy tree species etc.

Inactive Publication Date: 2011-04-20
嘉汉林业(广州)有限公司
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  • Abstract
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Problems solved by technology

[0005] However, generally speaking, although the existing chemical mutagenesis methods are relatively simple to operate, their mutagenesis efficiency and quality are still insufficient...

Method used

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  • Method for efficiently producing jatropha M_2 seeds processed by EMS (methanesulfonic acid ethyl ester)
  • Method for efficiently producing jatropha M_2 seeds processed by EMS (methanesulfonic acid ethyl ester)
  • Method for efficiently producing jatropha M_2 seeds processed by EMS (methanesulfonic acid ethyl ester)

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specific Embodiment approach

[0032] The invention provides a kind of method of producing Jatropha curcas M_2 seed, comprises the following steps:

[0033] The pretreatment step is to collect Jatropha curcas seeds, and carry out pretreatment to the seeds by chemical mutagenesis;

[0034] In the mutagenesis treatment step, the EMS solution of appropriate concentration is used to carry out chemical mutagenesis treatment on Jatropha curcas seeds for a suitable long time. During the chemical mutagenesis treatment process, stirring is also carried out at the same time to ensure that the concentration of the EMS solution is uniform and all Jatropha curcas seeds Can fully contact with EMS solution;

[0035] Seedling raising and transplanting steps, the Jatropha curcas M_1 seeds that have been treated with mutagenesis are carried out to grow seedlings and transplanted in time to cultivate Jatropha curcas M_1 plants. This step has no substantial difference with the corresponding steps in the existing Jatropha curca...

Embodiment 1

[0052] This embodiment specifically includes the following steps:

[0053] 1. Select 8000 wild seeds of Jatropha curcas collected in the same year in Yongren County, Chuxiong Prefecture, Yunnan Province, and cut off 3mm seed shells at the radicle end of each seed according to the aforementioned requirements.

[0054] 2. Clean the washing machine that has been improved according to the above requirements, put 14940ml of clean water and 60ml of EMS stock solution, and after rotating and stirring for 5 minutes, pour the seeds prepared in step 1 into the stirred EMS solution, rotate and stir for 2 minutes, and then set to automatic In the intermittent stirring mode, set the total operating time to 9 hours, and run and stir for 20 sec every 30 minutes, so that the seeds are fully impregnated with the EMS solution.

[0055] 3. After the automatic intermittent stirring mode is finished, discharge the EMS solution in time, put in 15000ml of clean water, stir for 5 minutes and then dis...

Embodiment 2

[0060] The processing step that present embodiment adopts is basically the same as embodiment 1, and its difference is that the Jatropha curcas seed collected in the pretreatment step of present embodiment is the provenance Jatropha curcas of Jin'an Town, Chulijiang City, Yunnan Province collected in the same year Wild Seeds.

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Abstract

The invention relates to a method for efficiently producing jatropha M_2 seeds processed by EMS (methanesulfonic acid ethyl ester), which comprises the following steps: a preprocessing step: collecting jatropha seeds, and preprocessing the jatropha seeds; a mutagenizing step: mutagenizing the jatropha seeds for an appropriate time by using an EMS solution (with appropriate concentration), and in the process of chemical mutagenizing, simultaneously mixing the seeds with the solution so as to ensure that the EMS solution is uniform in concentration and the jatropha seeds can be fully contacted with the EMS solution; a seedling raising and transplanting step: carrying out seedling raising and timely transplanting on the jatropha M_1 seeds subjected to mutagenizing so as to cultivate jatropha M_1 plants; and a self-pollination step: after the cultivated jatropha M_1 plants reach the flowering stage, carrying out self-pollination on the jatropha M_1 plants, after the fruits of the jatropha M_1 plants get ripe, obtaining the jatropha M_2 seeds. The method in the invention has the advantages that an ethylmethane sulfonate is used as a mutagen for mutagenizing the jatropha seeds, the preprocessing step and the mixing and self-pollination are adopted in the process of mutagenizing, the jatropha M_2 seeds processed by EMS can rapidly be obtained.

Description

technical field [0001] The present invention relates to the fields of plant mutagenesis and reproductive biology, in particular to a method using the alkylating agent ethyl methanesulfonate (abbreviated as "EMS" for short, and its molecular formula is CH 3 SO 2 OC 2 h 5 ) method for inducing mutations and efficiently producing Jatropha curcas M_2 seeds by treating them. Background technique [0002] Euphorbiaceae (Euphorbiaceae) Jatropha (Jatropha) Plant is a multi-purpose fast-growing tree species that is resistant to drought and barrenness. In my country, it is mainly distributed in Yunnan, Sichuan, Guangxi, Guangdong, Hainan and other areas with complex geological structures, serious soil erosion, serious mountain disasters, and fragile ecological environments. Jatropha curcas is the main raw material for the production of bio-energy, bio-pesticide, bio-medicine, and bio-feed. Research at home and abroad shows that Jatropha curcas seeds have a high oil content. In ...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H1/02A01C1/00
Inventor 杨建廖柏勇
Owner 嘉汉林业(广州)有限公司
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