Method for promoting jatropha curcas explant to regenerate adventitious buds

A technology of explants and Jatropha curcas, applied in the field of plant biology, can solve the problems of long regeneration cycle, low regeneration rate, poor quality of regeneration buds, etc., and achieves the effects of simple method, reduced negative impact, and shortened culture cycle.

Active Publication Date: 2013-12-11
嘉兴卓十生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a method for promoting Jatropha curcas explants to regenerate adventitious buds in order to overcome the defects of low

Method used

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  • Method for promoting jatropha curcas explant to regenerate adventitious buds
  • Method for promoting jatropha curcas explant to regenerate adventitious buds
  • Method for promoting jatropha curcas explant to regenerate adventitious buds

Examples

Experimental program
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Effect test

Embodiment 1

[0039] S1. Preparation of Vessels for Short-Term Cytokinin Solution Treatment of Explants

[0040] Select a petri dish with a diameter of 9 cm, place it in a high-pressure steam sterilizer, and sterilize it at 121 °C and 0.1 MPa for 20 min.

[0041] S2. Preparation of phenylthiadiazolyl urea (TDZ) treatment solution.

[0042] Accurately weigh a certain amount of TDZ powder, fully dissolve it with 1 mol / l NaOH solution, and then dilute it with deionized water to prepare 0, 10, 20, 30, 40, 50, 60 mg / l TDZ treatment solutions ( Control group 0 mg / l TDZ solution is sterile deionized water). Use 1 mol / l HCl solution to adjust the pH of the TDZ treatment solution to 5.8~6.0; filter and sterilize the prepared TDZ treatment solution with a 0.22 micron water filter before use.

[0043] S3. Acquisition of Jatropha curcas sterile hypocotyl explants

[0044] Jatropha curcas seeds soaked for 2 days were dehulled, sterilized by 0.1% mercuric chloride solution, and the embryos wer...

Embodiment 2

[0059] Example 2 The influence of the time of soaking in TDZ solution to treat Jatropha curcas sterile hypocotyl explants on the effect of direct regeneration of adventitious buds

[0060] S1. Preparation of the container for short-term cytokinin solution treatment of explants: same as in Example 1.

[0061] S2. Prepare a 20 mg / l phenylthiadiazolyl urea (TDZ) solution: the same as in Example 1.

[0062] S3. Acquisition of Jatropha curcas sterile hypocotyl explants: same as in Example 1

[0063] S4. Jatropha curcas sterile hypocotyl explants treated with cytokinin (TDZ) solution: the cut sterile hypocotyl explants were placed in a container treated with cytokinin (TDZ) solution, and the Pour the TDZ solution with a concentration of 20 mg / l into the wide-mouth glass bottle of the container until all the hypocotyl explants are submerged (0 mg / l TDZ solution of the control group is sterile deionized water), and cover the bottle cap , after soaking the hypocotyl explants ...

Embodiment 3

[0069] S1. Preparation of the container for short-term cytokinin solution treatment of explants: same as in Example 1.

[0070] S2. Prepare a 20 mg / l phenylthiadiazolyl urea (TDZ) solution, and the specific preparation method is the same as in Example 1.

[0071] S3. Acquisition of sterile hypocotyl explants of Jatropha curcas and hypocotyl explants of seedlings: the specific method is the same as that in Example 1.

[0072] S4. Cytokinin (TDZ) solution treated Jatropha curcas sterile hypocotyl explants and seedling hypocotyl explants respectively: the cut 2 types of hypocotyl explants were placed in cell division In the container treated with TDZ solution, pour TDZ solution with a concentration of 20 mg / l into the wide-mouth glass bottle of the container until all hypocotyl explants are submerged, cover the bottle cap, and test the hypocotyl explants. After soaking the axis explants for 20 min, pour off the TDZ solution, keep the hypocotyl explants, take out ...

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Abstract

The invention belongs to the technical field of plant biology, and particularly discloses a method for promoting a jatropha curcas explant to regenerate adventitious buds. The traditional jatropha curcas explant adventitious bud regeneration method is changed by the method, that is hormone is not added to a culture medium for inducing the adventitious buds to regenerate any longer, while a high-concentration thidiazuron solution is used for performing short-term soaking treatment on the jatropha curcas explants of various sources, and short-term and high-strength hormone stimulation is applied on explant cells, so that partial cells re-differentiate directly with higher efficiency to form more adventitious buds. The regeneration efficiency and the quality of the adventitious buds of the jatropha curcas explant can be improved significantly by using the method. In addition, the explant in the method is not required to be subjected to conventional caluus formation or an adventitious bud multiplication stage, so that a regeneration culture cycle can be shortened significantly, and the working efficiency of jatropha curcas related biotechnology breeding is improved correspondingly and greatly.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for promoting regeneration of adventitious buds from Jatropha curcas explants. Background technique [0002] With the growing global demand for fossil fuels and the depletion of stored fossil fuels, there is growing interest in renewable biodiesel. Among the candidate plants for biodiesel production, Jatropha curcas ( Jatroha curcas L.) has obvious advantages. Its seeds have high oil content, and the oil content of its kernels can reach 40%~60%. Pesticide and pharmaceutical value. [0003] However, vigorously promoting the planting of Jatropha curcas is facing a series of problems. Although the oil content in Jatropha curcas seeds is high, the seed yield is not high; there are many active ingredients in the seed oil, but the composition of the oil still needs to be changed to directly replace fossil fuels ; Jatropha curcas has high environmental requirements, weak ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘颖杨跃生刘振兰庄楚雄李静童欣惠文凯陈晓阳
Owner 嘉兴卓十生物科技有限公司
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