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Method for promoting seed germination of Kwangsi mayten herb

A seed, germination culture technology, applied in the direction of seed immunity, seed and rhizome treatment, application, etc., can solve problems such as failure to explore the interaction of different factors

Inactive Publication Date: 2014-04-16
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the existing studies focus on the chemical constituents and pharmacological effects of Maydenia plants, and there are few reports on the propagation and seedlings of Guangxi Maydenia, and the research on the germination of Guangxi Maydenia seeds is still blank; The few studies on seed germination in other species have studied the effect of a certain factor on the germination rate under specific conditions. This single-factor research method fails to explore the interaction between different factors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of culture substrate: The culture substrate is peat soil. Two weeks before use, spray and soak the culture substrate with 0.5% carbendazim solution, carry out disinfection treatment, and dry it under natural conditions for later use;

[0018] (2) Preparation of seeds: Wash the seeds of Guangxi Meidengmu with clean water, and select the sinking plump seeds by water selection method, and use 0.5% KMnO 4 Soak and sterilize for 30 minutes, rinse with clean water, and filter dry for germination;

[0019] (3) Seed soaking: Put the prepared seeds in a clean pot, add 3 mg / L gibberellin (GA 3 ) treatment solution, soak the seeds at room temperature for 12 h;

[0020] (4) Germination culture: put the prepared culture medium into flat-bottomed plates, soak it with distilled water, spread it out, and the thickness of the culture medium is about 2cm; spread the soaked seeds evenly on the culture medium, place Cultivate in an artificial climate incubator under th...

Embodiment 2

[0024] The culture medium is river sand; when soaking the seeds, add 1mg / L naphthaleneacetic acid (NAA) treatment solution 3 times the volume of the seeds, and soak the seeds at room temperature for 12 hours. All the other are the same as example 1.

[0025] Results: Germination began 20 days after sowing, reached the peak of germination at 35 days, and ended at 59 days. The germination rate was 60.0%, and the germination potential was 38.7%. The germination was irregular, and the radicle from the seeds was stronger.

Embodiment 3

[0027] The culture medium is river sand; when soaking the seeds, add distilled water 3 times the volume of the seeds, and soak the seeds at room temperature for 12 hours. All the other are the same as example 1.

[0028] Results: Germination began at 23 days after sowing, reached the peak at 39 days, and ended at 65 days. The germination rate was 30.7%, and the germination potential was 14.7%. The germination was irregular, and the radicle from the seeds was thinner.

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Abstract

The invention discloses a method for promoting seed germination of the Kwangsi mayten herb. The optimal external factor combination of the seed germination of the Kwangsi mayten herb is that a constant temperature is 25 DEG C; illumination intensity is 3,000lx and illumination time is 12h / d; a culture substrate adopts peat soil; growth regulator treating fluid adopts gibberellin (GA3) with the concentration of 3mg / L. Under the conditions, not only is the survival rate the highest and reaches 88.0 percent, but also indexes of germinability, an average root length, germinating performance, the healthy and strong degree of radicles and the like can achieve better effects. The method lays a good foundation for nursery stock breeding of the Kwangsi mayten herb and has very active influence on regeneration and continuous utilization of resources of medicinal plants, which are in imminent danger and in shortage.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for promoting the germination of Guangxi medeng seeds. Background technique [0002] Maydenia ( Maytenus ) plants belong to the Celastracae family and are perennial shrubs. The roots, stems and leaves of this genus contain anti-cancer active ingredients Maytansine and Maytanprrine, which have significant curative effects on breast cancer, lymphosarcoma, digestive tract cancer and other malignant tumors. In folklore, this genus of plants is widely used as anti-cancer, anti-septic, anti-septic, anti-inflammatory, and salivation-inducing drugs. Clinically, plants of the genus Maytans have anti-inflammatory, pain-relieving, detoxifying, and appetite-stimulating effects. Guangxi Meideng wood ( May tenus guangxiensis C. Y. Chenget W. L. Sha) is a medicinal plant unique to Guangxi karst. Due to the deterioration of the living environment and the indiscriminate logging by the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01C1/08
Inventor 陈宗游梁惠凌韦霄罗宝丽王满莲孔德鑫
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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