60results about How to "High in oleic acid" patented technology

The invention provides a method for preparing tea seed oil in a low-temperature cold pressing manner. The method comprises the following steps of cleaning tea seeds, removing shells and separating, milling oil at normal temperature, washing in water at low temperature, dehydrating under negative pressure, refining at low temperature, filtering for many times, winterizing and dewaxing. The method for preparing the tea seed oil in the low-temperature cold pressing manner, provided by the invention, has the advantages of simple process, low energy consumption and high oil yield; and the prepared tea seed oil has shallow color, high quality and comprehensive nutrition, and also keeps the original flavor of tea oil.

The invention discloses special blend oil for frying. The special blend oil for frying contains 10-50% by weight of high oleic acid peanut oil and 10-50% by weight of rice oil. The blend oil for frying is prepared by compounding any two or more than two of sunflower seed oil, olive oil, soybean oil, corn oil, cottonseed oil, palm oil, rapeseed oil, safflower oil, linseed oil and sesame oil in different proportions. The blend oil for frying is rich in fragrance, bright in color, high in content of oleic acid, rich in oryzanol and vitamin E, reasonable in composition of fatty acids and free of stickiness. Through a high-temperature frying test, the blend oil is high in smoke point, low in content of trans-fatty acids and high in frying stability. The fried food is small in oil content, good in crispy property, excellent in taste and nutritional and healthy. The blend oil disclosed by the invention can be used for frying different food materials, is suitable for being used as special fat for frying food in family and catering industry, is long in expiration date, and is novel nutritional and healthy special blend oil for frying.

The invention discloses a culture method for improving the oleic acid content of peanuts. The method comprises the following steps: selecting a new variety of peanuts with high oleic acid; sowing in an optimum period; covering with a black-and-white plastic film; additionally applying an organic fertilizer, inorganic fertilizer and coated control-releasing fertilizer for mixed use; spraying foliar fertilizer in a later period; and adopting a chemical adjusting method and an irrigation method to increase soil temperature. The method is utilized to plant peanuts so as to improve the per-mu yield of the peanuts by 20-40 kg, improve the oleic acid content of peanuts by 3-10 percent and have favorable economic benefits and social benefits.

The invention belongs to the technical field of traditional Chinese medicine, and relates to brucea javanica oil dry emulsion capsule and a preparation process thereof. The brucea javanica oil dry emulsion capsule takes brucea javanica oil as an active ingredient, and also comprises a pharmaceutically acceptable carrier and an accessory, the brucea javanica oil is added in micro emulsion manner, the accessory is lactose, the carrier is HPMC (Hydroxy Propyl Methyl Cellulose), the mass ratio of the HPMC to the lactose is (7-8):1, the mass ratio of the total mass of the HPMC and the Lactose to the micro emulsion of the brucea javanica oil is 1:(6-8); the micro emulsion of the brucea javanica oil consists of an oil phase, a surface active agent, a cosurfactant and distilled water, the oil phase comprises MCT (Medium Chain Triglyceride) and brucea javanica oil, and the cosurfactant is absolute ethyl alcohol. The brucea javanica oil dry emulsion capsule prepared by the invention is stable in physicochemical property, and is shaped like ovoid when being observed by using a transmission electron microscope after being redissolved, and the particle size is between 15-40nm. The thin-layer chromatography identification is adopted, and the specificity of the method is good. The oleic acid content in the dry emulsion is not less than 13.5% according to the measurement of the gas chromatography method, and the drug content in a sample is uniform.

The invention discloses a nucleotide mutational site for indicating high oleic acid content of rapeseeds and relates to the field of plant molecular seed breeding. When any nucleotide mutational site exists in a certain variety (strain) of the rapeseeds, the variety (strain) has a high oleic acid content property; when two nucleotide mutational sites exist at the same time, higher oleic acid content is indicated. According to the nucleotide mutational site, one rape high oleic acid mutant is used as a material, and PCR (Polymerase Chain Reaction) amplification is carried out on a rape oleic acid desaturase gene (BnFAD2), so as to obtain two high oleic acid mutational sites in the mutant. The high oleic acid rape variety (strain) containing the mutational sites is hybridized with a common variety (strain), and the mutational sites are detected in a subsequent separation generation strain, and the rape variety (strain) with the oleic acid content which is 85 percent can be efficiently and accurately obtained.

The invention belongs to the technical field of genetic crop gene engineering, and particularly relates to a construction method of cabbage type rape gene mutant PTG8 and an application of the construction method. The technical scheme includes that the construction method includes the steps: designing and screening target sequence of two base sites according to gene nucleotide sequences of cabbage type rape BnaC.FAD2.a and BnaA.FAD2.a, and designing primers according to the target sequence; constructing a double-target gene editing BnPTG8 vector; performing genetic transformation on the BnPTG8 vector and acceptor materials to obtain the gene mutant. According to the construction method, a plurality of different sources of cabbage type winter rape strains are screened, an efficient and stable transformation gene acceptor material ZP1 is acquired and accurately edited to obtain a BnaC.FAD2.a single-gene mutant and a double-gene mutant of the BnaC.FAD2.a and the BnaA.FAD2.a, the foundation is laid for building of an efficient winter rape genetic transformation technology platform, and a new germplasm resource is provided for improvement of cabbage type rape fatty acid.

The invention relates to the technical field of peanut breeding, in particular to a breeding method of a high oleic acid peanut germplasm. The breeding method comprises the following steps: (1) a female parent 1 and a male parent 1 are hybridized, and then successive generation selfing and oriented screening are performed to obtain 8-153 high oleic acid strains with high oleic acid content not less than 80%; (2) the 8-153 high oleic acid strains and a male parent 2 are hybridized, and then successive generation selfing and oriented screening are performed to obtain 11 high oleic acid strains with high oleic acid content not less than 80%; (3) the 11 high oleic acid strains and a male parent 3 are hybridized, and then successive generation selfing and oriented screening are performed to obtain the novel high oleic acid peanut germplasm. The novel peanut germplasm bred by a breeding technique has the characteristics of high oleic acid content, low linoleic acid content, high yield, higher kernel percent, bacterial wilt resistance, leaf spot resistance and rust resistance.

The invention discloses a perfuming, anti-corrosion and anti-bacterium processing technology for an ox bone folding fan framework, and belongs to the field of folding fan bamboo material processing technologies. The perfuming, anti-corrosion and anti-bacterium processing technology for the ox bone folding fan framework is characterized by comprising the steps of preparation of anti-bacterium anti-corrosion solvent and anti-bacterium anti-corrosion perfume, material boiling, baking, spraying and slurry wrapping. In addition, due to the fact that a surfactant, detergent dispersant and sodium carboxy methyl cellulose are added to the anti-bacterium anti-corrosion solvent, all anti-corrosion anti-bacterium components can be mixed together to be consistent, and the anti-bacterium anti-corrosion effect can be maximized and optimized under the synergistic action; due to the fact that staged baking perfuming operation is conducted on the ox bone folding fan framework through pure natural plant perfume materials, the fragrance can slowly penetrate into ox bone fibers, the smoking flavor cannot be generated, and the perfuming fragrance is durable and fresh; after a high-oleic ox bone preservative solution is sprayed, the surface of the ox bone folding fan framework is repeatedly wiped through cotton cloth and chamois leather until a slurry wrapping layer in a glossy state exists, an anti-oxidation layer on the surface can be densified, and the weather resistance is effectively improved.

The invention discloses a mosquito driving and antiseptic antibacterial processing technology for folding-fan bamboo ribs and belongs to the field of folding-fan bamboo material processing technologies. The technology is characterized by including the steps of preparation of an antibacterial antiseptic solution and an antibacterial antiseptic cooking material, material cooking, spraying and smoke curing. Besides, antiseptic antibacterial ingredients are enabled to be fused consistently by adding a surface active agent, purification dispersant and sodium carboxymethylcellulose into the antibacterial antiseptic solution, and antibacterial antiseptic effect is maximized and optimized under cooperative action. The antibacterial antiseptic solution and the antibacterial antiseptic cooking are selected from pure natural materials, purchase cost is economical and practical, mosquito driving and antiseptic antibacterial effect is good, no harmlessness is caused to environment and human beings, success is achieved via experimental stages and batch tests, and the technology is suitable for popularization in trade unions.

The invention discloses a high oleic and high yield peanut breeding method, and belongs to the field of plant breeding. The method comprises the following steps that (1), high yield peanut varieties are selected as a female parent, high-oleic peanut varieties are selected as a male parent, hybridization is conducted, and a high yield and high-oleic F1 generation is obtained; (2), the F1 generationis subjected to selfing after false hybrids is removed, and an F2 generation is obtained; (3), high-oleic and high yield single plants are screened out, selfing is conducted, and an F3 generation isobtained from the F2 generation; (4), stable high oleic and high yield single plants are screened out from the F3 generation and subjected to backcross with the high yield peanut parent in step (1), and an F4 generation is obtained; (5), high oleic and high yield single plants are screened out from the F4 generation and subjected to backcross with a high oleic peanut male parent, and an F5 generation is obtained; (6), F5 is subjected to selfing for four consecutive generations, an F6 generation, an F7 generation, an F8 generation and an F9 generation are obtained, and the F8 generation is thenew variety of the high oleic and high yield peanut. By means of the method, the new peanut variety of the peanut high in yield and oleic acid content is bred.

The invention discloses a method for obtaining high-oleic-acid cotton by applying a gene editing technology. According to the method, firstly, gRNA targets including sgRNA1 and sgRNA2 are selected, then a pRGEB32-GhU6.9-NPTII carrier is used as a basis, and the fused sgRNA1 and sgRNA2 are inserted into a BasI locus of the pRGEB32-GhU6.9-NPTII carrier; the established carrier is converted into competence escherichia coli, a culture medium slab with kanamycin is coated with the competence escherichia coli for screening, a single colony is selected for culture, PCR identification is conducted, amonoclone with correct sgRNA1 and sgRNA2 sequences is cultured, plasmid is extracted, an agrobacterium-mediated mode is adopted, the plasmid is converted into cotton, a transgenic cotton plant with FAD2 gene mutation is obtained through screening, the oleic acid content of transgenic cotton seeds is obviously increased, and the linoleic acid content of the transgenic cotton seeds is obviously lowered.

The invention discloses camellia oil soap powder and a preparation method thereof. The camellia oil soap powder is prepared from the following components in parts by weight: camellia oil saponin, sodium hydroxide, sodium chloride and a compound, wherein the ratio of the four substances is equal to 100 to (1 to 3) to 100 to 110; the compound is a mixture of sodium carbonate, sodium polyacrylate, fatty alcohol polyoxyethylene ether sodium, talcum powder, sodium citrate, ethylenediamine tetraacetic acid, sodium dodecyl benzene sulfonate and essence; the weight ratio of sodium carbonate to sodiumpolyacrylate to fatty alcohol polyoxyethylene ether sodium to the talcum powder to sodium citrate to the ethylenediamine tetraacetic acid to sodium dodecyl benzene sulfonate is 6 to 1 to 0.5 to 2 to 0.5 to 0.5 to 0.5; and the adding amount of the essence is 0.1 thousandth of the camellia oil soap powder. The camellia oil soap powder has obvious decontamination ability and an excellent washing effect on carbon inks, proteins and grease stains; and in addition, the camellia oil soap powder has the advantages of easiness in rinsing, small irritation to skin, good environmental protection effect and low cost.

The invention firstly discloses nucleotide sequence of mutant gene delta12-fatty acid dehydrogenase gene (AhFAD2') which leads oleic acid content of peanut variety E11 and E16 to increase and shortened protein sequence which is coded. The nucleotide sequence also provides a clone method of the peanut fatty acid dehydrogenase mutant gene. The expression analysis of mRNA of gene AhFAD2' of the invention shows that the gene can express in function.

The invention belongs to the technical field of gene engineering, and particularly relates to a preparation method of a peanut mutant, a peanut mutant gene, protein encoded by the peanut mutant gene and an application. By designing SgRNA T1-T4, a CRISPR/Cas9 genome editing system is constructed, a Cas9 enzyme guided by sgRNA cuts a peanut desaturated fatty acid enzyme gene AhFAD2A at a gene target, and gene sequence insertion or base mutation is generated after DNA repair, so that targeted mutation of the peanut desaturated fatty acid enzyme gene AhFAD2A is completed, the gene cannot synthesize a functional desaturated fatty acid enzyme and cannot catalyze oleic acid dehydrogenation to generate linoleic acid, peanuts with high oleic acid content can be obtained, and the quality of peanut varieties can be improved.

The invention belongs to the field of biotechnology, and relates to a gene improving technique for improving nutritional quality of rapeseed oil by co-inhibiting rape FAD2 and FAE1 gene expression. According to the method, it creates FAD2 and FAE1 single-gene and double-gene RNA interference materials, namely FAD2-Ri, FAE1-Ri and FAD2/FAE1-Ri, which are under the control of a seed specific expression promoter napin A. The interference materials show obviously reduced FAD2 and FAE1 gene expression, however growth and development processes are not affected. Fatty acid fingerprinting of the rapeseed oil is modified by the inhibited FAD2 and FAE1 gene expression, resulting in obvious increase in an oleic acid content and quite obvious decrease in an erucic acid content in interference material seeds, and consistently, C18: 1/C18: 2 and C18: 1/C18: 3 ratios in C18 unsaturated fatty acid of transgenic rapeseed oil are greatly improved. Furthermore, increase in a protein content of rape seeds to some degree is also caused by the reduced FAD2 and FAE1 gene expression.

The invention discloses an externally-used traditional Chinese medicine ointment for treating burns and scalds and a preparation method thereof. The ointment is composed of raw materials and auxiliary materials, wherein the raw materials are composed of, by weight, 40-60 parts of catechu, 40-60 parts of common bletilla tubers, 40-60 parts of radix sanguisorbae, 80-120 parts of radix scutellariae, 80-120 parts of pubescent holly roots, 4-6 parts of borneol and 750-850 parts of chicken grease. The externally-used traditional Chinese medicine ointment is a folk recipe based on the theory of traditional Chinese medicine, has the effects of clearing heat, removing toxicity, achieving astringency, stopping pain, removing swellings and promoting granulation and can effectively relieve pain of the burns and scalds and facilitate healing and regenerating of wound surfaces, the preparation technology is simple, the production cost is low, and use is convenient. The externally-used traditional Chinese medicine ointment has a significant therapeutic effect on first-degree and slight second-degree burns and scalds such as boiled water scalds, fire burns, electric burns and drug stimulation.

The invention discloses a method for improving rice variety characteristics through microgravity. The method comprises the following steps: S1, seeds are put into a microgravity device, the microgravity device rotates at the speed of 10 to 15 r/min for 24 to 36 h in the first direction, and the pretreated seeds are obtained, S2, the pretreated seeds are accelerated, the microgravity device is adjusted to rotate at the speed of 60 to 80 r/min for 240 o 360 h in the first direction, and activated seeds are obtained; and S3, the activated seeds are decelerated and rotated at the speed of 10 to 15r/min for 24 to 35 h in the second direction, and improved seeds are obtained, wherein the second direction is opposite to the first direction. Progeny seeds of the rice seeds obtained through the method show that the total amount of amino acids is increased, the protein content is increased, the sodium ion content is greatly increased, and the oleic acid content of certain varieties is increased, so that the quality is improved, the growth period of rice varieties is shortened, and the yield is increased.

The invention discloses antimicrobial antiseptic cooking liquid for folding-fan bamboo ribs and an antimicrobial antiseptic technology of the antimicrobial antiseptic cooking liquid and belongs to the field of folding-fan bamboo material processing formula and technologies. The antimicrobial antiseptic cooking liquid is characterized by comprising an antimicrobial antiseptic solution and an antimicrobial antiseptic cooking material. Besides, antiseptic antibacterial ingredients are enabled to be fused consistently by adding a surface active agent, purification dispersant and sodium carboxymethylcellulose into the antibacterial antiseptic solution, and antibacterial antiseptic effect is maximized and optimized under cooperative action. The antibacterial antiseptic solution and the antibacterial antiseptic cooking are selected from pure natural materials, purchase cost is economical and practical, mosquito driving and antiseptic antibacterial effect is good, no harmlessness is caused to environment and human beings, success is achieved via experimental stages and batch tests, and the technology is suitable for popularization in trade unions.

A preparation method of an alkaline oleate detergent, comprising the following steps: adding an organic solvent, an alcohol accelerator, calcium hydroxide or calcium oxide and water into a flask, turning on the stirrer, and raising the temperature to 30-40°C, After stirring evenly, add oleic acid and base oil to carry out neutralization reaction, maintain the reaction temperature at 40-60°C, and react for 0.5h-1h; , maintain the reaction temperature at 40-60°C; after the high alkalinity reaction, raise the temperature of the mixture to 110-130°C to remove the alcohol accelerator and water, then cool down to 60-80°C, add an organic solvent to dilute and centrifuge to remove The slag is then distilled under reduced pressure to remove the organic solvent to obtain the product, which is stable at high temperature, has no biological toxicity and has good environmental compatibility, excellent high temperature detergency, good storage stability, abrasion resistance and water separation.

The invention discloses an antiseptic and anti-microbial processing process of folding fan bamboo ribs capable of releasing negative ions and belongs to the field of folding fan bamboo material processing processes. The antiseptic and anti-microbial processing process is characterized by comprising the steps of preparing an antiseptic and anti-microbial solvent and an antiseptic and anti-microbial spice, forming slotted holes, boiling the materials, performing spraying, manufacturing negative-ion insertion strips, embedding the insertion strips and performing slurry wrapping. In addition, a surface active agent, a dispersant and sodium carboxymethyl cellulose are added into the antiseptic and anti-microbial solvent, so that all antiseptic and anti-microbial components are compatible consistently, and the antiseptic and anti-microbial effects are maximized and optimized under the synergistic effect. By means of the antiseptic and anti-microbial processing process, the folding fan bamboo ribs can be effectively subjected to antiseptic treatment and can be durable in use, the material performance index of the bamboo ribs is greatly improved, and the folding fan bamboo ribs have antiseptic and health-care effects and can diffuse negative ions beneficial to human bodies.

The invention relates to a gene mutant for improving soybean oleic acid content and application thereof, and belongs to the technical field of plant gene engineering. The gene mutant is a single-genemutant or a polygene mutant; the single-gene mutant is subjected to FAD2-1A gene mutation; the polygene mutant is subjected to joint mutation of an FAD2-1A gene and other genes, and the other genes are one or two of an FAD2-1B gene and an FAD3A gene; FAD2-1A gene mutation is that two bases are deleted at the 529th site and the 530th site of the gene; the FAD2-1B gene mutation is that 10 basic groups are deleted at the 265-274 site of the gene; the FAD3A gene mutation is that seven basic groups are deleted at the 55-61th site of the gene. The gene mutation site information provides a brand-new way for quality breeding work of soybeans or other oil crops with high oleic acid content, and has a good application prospect.

The invention relates to a soybean Gm-SEIPIN2 family gene and application of the soybean Gm-SEIPIN2 family gene in increasing the oil content of plant seeds, belonging to the technical field of gene engineering. The soybean Gm-SEIPIN2 family gene comprises two genes, namely, a Gm-SEIPIN2A gene and a Gm-SEIPIN2B gene. The soybean Gm-SEIPIN2A gene and the soybean Gm-SEIPIN2B gene of soybean Gm-SEIPIN2 family gene are subjected to functional verification by using a yeast mutant transformation technology and a transgenic Arabidopsis thaliana technology; the results of oil content determination ofand fatty acid ratio determination through a gas chromatography technology prove that the Gm-SEIPIN2A gene and the Gm-SEIPIN2B gene can improve the oil content to different degrees; the results of determination of fatty acid ratios in yeasts show that the above two genes can improve the content of oleic acid; and Arabidopsis oil content determination results show that the above two genes can obviously improve the oil content of Arabidopsis seeds and obviously reduce the content of palmitic acid. The two genes of the Gm-SEIPIN2 family are of great significance in increasing the oil content of plant seeds and increasing the oleic acid content, especially in cultivating high-oil and high-oleic-acid soybean varieties.