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Peanut delta12-fatty acid dehydrogenase mutant gene and its coding protein and clone method

A technology of fatty acid dehydrogenase and mutant gene, which is applied in peanut fatty acid dehydrogenase mutant gene and its encoded protein and cloning field, which can solve the problems of unhealthy health and lower beneficial cholesterol content, etc.

Inactive Publication Date: 2008-08-06
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Linoleic acid is a polyunsaturated fatty acid, although it can reduce bad cholesterol, it can also reduce the content of good cholesterol, so it is not good for health.

Method used

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Examples

Experimental program
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Embodiment Construction

[0065] The present invention will be described in further detail below in conjunction with specific examples.

[0066] Select the seeds (not more than 0.1 g) of high-oleic peanut variety E11 or E16 in a mortar, add 0.5 ml plant RNA extraction reagent (RNAplant reagent from Tianwei Times Company) at room temperature, grind thoroughly, and transfer to 1.5 mL EP In the tube, shake until thoroughly mixed, extract the total RNA, use formaldehyde denaturing gel electrophoresis to identify the quality of the total RNA, and then measure the RNA content on a spectrophotometer. The obtained total RNA was used as a template, and 5'-RACE-Ready cDNA and 3'-RACE-Ready cDNA were synthesized by reverse transcription, and the 5'-end sequence and 3'-end sequence of the fatty acid dehydrogenase mutant gene were obtained by RACE technology. Design primers at both ends according to the 5' end sequence and 3' end sequence of the fatty acid dehydrogenase mutant gene

[0067] FADf5: 5'-ACTCACTTCTCTT...

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Abstract

The invention firstly discloses nucleotide sequence of mutant gene delta12-fatty acid dehydrogenase gene (AhFAD2') which leads oleic acid content of peanut variety E11 and E16 to increase and shortened protein sequence which is coded. The nucleotide sequence also provides a clone method of the peanut fatty acid dehydrogenase mutant gene. The expression analysis of mRNA of gene AhFAD2' of the invention shows that the gene can express in function.

Description

technical field [0001] The invention belongs to the field of fatty acid metabolism engineering, and in particular relates to a mutant gene of peanut fatty acid dehydrogenase, its encoded protein and a cloning method. Background technique [0002] Peanut is an important oil crop, rich in high-quality protein and fat, and it is a good food and health product for people to keep fit and increase their lifespan. The oil content in peanut kernels is 45-51%, of which about 80% is composed of oleic acid and linoleic acid. Oleic acid plays a special role in the lipid metabolism of the human body. It can reduce bad cholesterol (low-density lipoprotein, LDL), but maintain the level of good cholesterol (high-density lipoprotein, HDL), thereby slowing down atherosclerosis , Effectively prevent the occurrence of cardiovascular diseases such as coronary heart disease. And linoleic acid is a polyunsaturated fatty acid, although it can reduce bad cholesterol, it can also reduce the content...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12N9/04
Inventor 禹山林曹玉良闵平杨庆利潘丽娟
Owner SHANDONG PEANUT RES INST
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