Nucleotide mutational site for indicating high oleic acid content of rapeseeds

A mutation site, nucleotide technology, applied in the field of nucleotide mutation sites

Inactive Publication Date: 2017-01-04
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But unfortunately, the content of rapeseed oleic acid corresponding to these two molecular markers is only 76-77%

Method used

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  • Nucleotide mutational site for indicating high oleic acid content of rapeseeds
  • Nucleotide mutational site for indicating high oleic acid content of rapeseeds
  • Nucleotide mutational site for indicating high oleic acid content of rapeseeds

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Embodiment Construction

[0038] The methods used in the following embodiments are conventional methods unless otherwise specified.

[0039] 1 Search for and obtain candidate indicator sites for high oleic traits in Brassica napus.

[0040] First of all, it needs to be explained that the material we use is rapeseed high oleic acid (oleic acid content > 85%) mutant N1379T (publicly known and public) with independent intellectual property rights, patent application number: 201010513722.9, preserved in the China Committee for Microorganism Culture Collection General Microbiology Center, preservation number CGMCC NO.4106) and common oleic acid content (oleic acid content is about 65%) Brassica napus variety Ningyou No. 14 (publicly known and public, nationally approved rapeseed variety, approval number: National Examination You 2004024 ).

[0041] The synthesis and degradation pathways of vegetable oleic acid are relatively clear. There are two synthetic pathways for oleic acid: one is to use stearic aci...

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Abstract

The invention discloses a nucleotide mutational site for indicating high oleic acid content of rapeseeds and relates to the field of plant molecular seed breeding. When any nucleotide mutational site exists in a certain variety (strain) of the rapeseeds, the variety (strain) has a high oleic acid content property; when two nucleotide mutational sites exist at the same time, higher oleic acid content is indicated. According to the nucleotide mutational site, one rape high oleic acid mutant is used as a material, and PCR (Polymerase Chain Reaction) amplification is carried out on a rape oleic acid desaturase gene (BnFAD2), so as to obtain two high oleic acid mutational sites in the mutant. The high oleic acid rape variety (strain) containing the mutational sites is hybridized with a common variety (strain), and the mutational sites are detected in a subsequent separation generation strain, and the rape variety (strain) with the oleic acid content which is 85 percent can be efficiently and accurately obtained.

Description

technical field [0001] The invention relates to a nucleotide mutation site indicating the high oleic acid content of rape seeds, belonging to the field of plant molecular breeding, in particular to the detection and utilization of the nucleotide mutation site which can indicate the trait of high oleic acid content of rapeseed. More specifically, it relates to the isolation of high oleic acid gene nucleic acid sequence of Brassica napus, the discovery of high oleic acid mutation sites and its detection method, and the molecular breeding of rapeseed varieties (lines) with high oleic acid traits. Background technique [0002] Brassica napus is an important oil crop in the world. Rapeseed oil is a traditional edible oil in China and the largest source of domestic edible vegetable oil. The quality of rapeseed oil has an important influence on the healthy consumption of oils and fats in my country (Yin Yan, et al., China Agricultural Science and Technology Herald, 2010(3): 16-21)....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/11C12Q1/68
CPCC12N9/0071C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 龙卫华浦惠明高建芹胡茂龙张洁夫陈松戚存扣
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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