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Molecular marker related to photoperiod and thermo-sensitive genic male sterile phenotype of rice and application

A molecular marker and male sterility technology, applied in the field of plant molecular biology, can solve the problem of little understanding of the molecular basis, and achieve the effect of improving the efficiency of breeding

Active Publication Date: 2020-05-05
HAINAN BOLIAN RICE GENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many rice photothermosensitive genic male sterile genes have been mapped or cloned, the molecular basis for the regulation of rice photothermosensitive genic male sterility traits is still poorly understood

Method used

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  • Molecular marker related to photoperiod and thermo-sensitive genic male sterile phenotype of rice and application
  • Molecular marker related to photoperiod and thermo-sensitive genic male sterile phenotype of rice and application
  • Molecular marker related to photoperiod and thermo-sensitive genic male sterile phenotype of rice and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Phenotype and genetic analysis of rice photothermo-sensitive genic male sterile mutant tms3193

[0044] 1) Screening of tms3193 mutants

[0045] In June 2013, 10 kg of 93-11 seeds were irradiated with cobalt 60 to obtain the M0 generation. The irradiated seeds were planted in the experimental field in Lingao County, Hainan Province, and harvested by individual plants after maturity. A total of about 6,500 M1 generation materials were obtained. In the spring of 2014, 3617 M1 generation materials with a large amount of seeds were selected and planted into plant lines, each planting 50 individual plants. Various types of mutants such as plant type, ear type, fertility, yield, etc. were screened at the tillering stage, booting stage, heading stage, flowering stage, and filling stage, and harvested and stored. One mutant was found to be male sterile in the 3193 family, and was named tms3193.

[0046] 2) Phenotypic analysis of tms3193 mutant

[0047] Under the high seaso...

Embodiment 2

[0050] Example 2 Preliminary localization of TMS3193 photo-temperature-sensitive male sterility gene

[0051] Using bulked segregant analysis and recessive-class analysis to locate the TMS3193 gene. A F2 population was constructed by crossing Minghui 63 as the male parent with the tms3193 mutant, and 205 sterile plants were isolated. 301 pairs of SSR markers evenly distributed on 12 chromosomes were used to screen the markers with polymorphism between 93-11 and Minghui 63, and 64 pairs of SSR markers with polymorphism were obtained. The polymorphism was low, indicating that Hui 63 and 93-11 have little difference in genome sequence. A mixed gene pool of fertile plants and sterile plants of the F2 population is constructed separately, and the fertile gene pool and the sterile gene pool are analyzed using the 64 pairs of polymorphic markers. The results showed that SSR markers RM243 and RM023 on chromosome 1 were linked to the mutation phenotype of tms3193. The above-mentioned l...

Embodiment 3

[0052] Example 3 Fine Mapping of TMS3193 Photo-temperature Sensitive Male Sterility Gene in Rice

[0053] On the basis of the above coarse positioning results, a recessive-class analysis method is used for fine positioning. Using linkage markers to select tms3193 heterozygous individual plants in the F2 population developed an F3 population containing 3322 individual plants, of which 904 sterile plants were isolated. At the same time, 5 polymorphic markers were further developed between the markers RM243 and RM10526. Among them, the primer used to detect the molecular marker Indel14420 is shown in SEQ ID NO.1-2; the primer used to detect the molecular marker M3193-2 is shown in SEQ ID NO.3-5; the primer used to detect Indel14430 is shown in SEQ ID NO. IDNO.6-7 shown. Using these markers to detect the genotypes of the sterile plants isolated from the F3 population one by one, it was found that only one sterile plant had expanded 105bp and 111bp bands at the M3193-2 marker, while...

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Abstract

The invention provides a molecular marker related to a photoperiod and thermo-sensitive genic male sterile phenotype of rice and application and belongs to the technical field of gene engineering. Byusing a map-based cloning method, TMS3193 is finely positioned on a No.1 chromosome and in a section of 28.76Kb between molecular markers M3193-2 and Indel14430, and the nucleotide sequence of the section is shown in SEQ ID NO.8. The gene is in coseparation with a marker Indel14420, and is in close linkage with the markers M3193-2 and Indel14430. Because of mutation of TMS3193, rice has a photoperiod and thermo-sensitive phenotype of long-day high-temperature sterility and short-day low-temperature fertility. Due to fine positioning of tms3193 and development of coseparation or close linkage markers, novel sterile gene pools and molecular assistant selection means are provided for breeding of a photoperiod and thermo-sensitive two-line sterile line of rice, and great application potentialcan be achieved in breeding of two-line sterile lines.

Description

Technical field [0001] The invention belongs to the field of plant molecular biology, and specifically relates to molecular markers and applications related to the phenotype of rice photothermo-sensitive nuclear male sterility. Background technique [0002] Rice is the staple food for more than half of the world’s population. Increasing rice production will help humans get rid of hunger. Hybrid rice takes advantage of the heterosis phenomenon of rice, which greatly improves the yield, resistance and adaptability of rice. Since Mr. Yuan Longping proposed to use the male sterility of rice to produce hybrid rice in the 1960s, hybrid rice in China has experienced a development from three-line to two-line hybrid rice. Three-line hybrid rice uses nucleocytoplasmic sterile lines for hybrid rice breeding, while two-line hybrid rice uses light-temperature-sensitive sterile lines for hybrid rice breeding. Compared with the three-line sterile line, the two-line sterile line has a wide ran...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 龙湍唐杰李佳林刘昊韩晓斌曾翔吴永忠黄培劲
Owner HAINAN BOLIAN RICE GENE TECH CO LTD
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