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Molecular marker related to rice nucleus male sterility phenotype and application

A molecular marker and male sterility technology, applied in the field of plant molecular biology, can solve the problems of high risk of seed production, fertility affected by light and temperature environment, and geographical restrictions of seed production, so as to improve the breeding efficiency, efficient and accurate screening and other problems. The effect of detection

Pending Publication Date: 2021-01-08
HAINAN BOLIAN RICE GENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the fertility of this type of sterile line is affected by the light and temperature environment, it also leads to high risk of seed production, and the area of ​​seed production is limited

Method used

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  • Molecular marker related to rice nucleus male sterility phenotype and application
  • Molecular marker related to rice nucleus male sterility phenotype and application
  • Molecular marker related to rice nucleus male sterility phenotype and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Phenotype and genetic analysis of rice nuclear male sterile mutant gms4372

[0055] 1) Screening of gms4372 mutants

[0056] In June 2013, 10 kg of 93-11 seeds were irradiated with cobalt 60 to obtain the M0 generation. The irradiated seeds were planted in the experimental field of Lingao County, Hainan Province, and harvested by individual plants after maturity. A total of about 6,500 M1 generation materials were obtained. In the spring of 2014, 3617 M1 generation materials with a large amount of seeds were selected and planted into strains, and 50 individual plants were planted in each strain. Various types of mutants such as plant type, panicle type, fertility, yield, etc. were screened at the tillering stage, booting stage, heading stage, flowering stage, and grain filling stage, and harvested and stored. In family No. 4372, a mutant was found to be male sterile, and it was named gms4372.

[0057] 2) Phenotype analysis of gms4372 mutant

[0058] Under ...

Embodiment 2

[0061] Example 2 Preliminary Mapping of the Male Sterility Gene GMS4372

[0062] The GMS4372 gene was mapped using bulked segregant analysis and recessive-class analysis for extreme traits. A F2 population was constructed by crossing Minghui 63 with the gms4372 mutant, and 59 sterile plants were isolated. Using 301 pairs of SSR markers evenly distributed on 12 chromosomes to screen polymorphic markers between 93-11 and Minghui 63, 64 pairs of polymorphic SSR markers were screened, and the polymorphism was low, indicating that The genome sequences of Hui 63 and 93-11 were slightly different. The mixed gene pools of the fertile and sterile plants of the F2 population were respectively constructed, and the fertile gene pool and the sterile gene pool were analyzed by using the 64 pairs of polymorphic markers. The results showed that there was a linkage relationship between the SSR marker RM263 on chromosome 2 and the mutant phenotype of gms4372. The genotypes of the sterile str...

Embodiment 3

[0066] Example 3 Polymorphisms of RM13592, RM13599 and RM13606 in Different Varieties

[0067] In order to verify the specificity of the three markers RM13592, RM13599 and RM13606 in indicating the male sterility phenotype of the gms4372 mutant, the polymorphisms of the three markers in multiple varieties were analyzed, wherein RM13592 consists of SEQ ID NO.1-2 The primers shown are amplified, RM13599 is amplified by the primers shown in SEQ ID NO.3-4, and RM13606 is amplified by the primers shown in SEQ ID NO.5-6. Figure 6 Swimming lanes 1-10 show that RM13592 amplified bands of 105 bp different from the size of the parent gms4372 in other parents, except for the 105 bp bands amplified in gms4372, H28B, Q3B, Zhongzhe B and Wufeng B. Figure 6 Lanes 11-19 show that in the F1 hybrids of gms4372 and other parents, in addition to the above-mentioned H28B, Q3B, Zhongzhe B and Wufeng B donors, the bands of two parents can be amplified simultaneously with other parents, indicating ...

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Abstract

The invention relates to the technical field of plant molecular biology, in particular to a molecular marker related to a rice nucleus male sterility phenotype and an application. Through a map-basedcloning method, a gene GMS4372 is positioned in a segment of 1669.1 Kb between a molecular marker RM13562 and a molecular marker RM13630 on a long arm of a second chromosome, besides, the invention also finds that the segment is co-separated from the markers RM13592, RM13599 and RM13606 and is closely linked with the markers RM13562 and RM13630. The mutation of GMS4372 enables rice to show male sterility phenotype. Discovery of the GMS4372 and development of markers which are co-separated from or closely linked with the GMS4372 provide a new sterile gene source and a molecular assistant selection means for breeding of the male sterile line of rice, and have huge application potential in breeding of the male sterile line.

Description

technical field [0001] The invention belongs to the technical field of plant molecular biology, and specifically relates to molecular markers and applications related to rice nuclear male sterility phenotype. Background technique [0002] Rice is one of the most important food crops in the world. With the growth of population and the improvement of quality of life, it is estimated that by 2050, the annual output of rice will increase by 1-2 times to meet the needs of human development. Hybrid rice is the generation of offspring obtained by crossing parents. Its yield is often 15% higher than that of conventional rice parents, and its resistance and adaptability are far better than the parents. Therefore, the application and popularization of hybrid rice is an important way to increase rice yield. [0003] The male sterile line is a key node in hybrid rice breeding technology. Male sterile lines refer to plant strains with abnormal development of male gametes and loss of f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCC12Q1/6895A01H1/04C12Q2600/13C12Q2600/156
Inventor 唐杰吴春瑜李佳林曾翔吴永忠黄培劲
Owner HAINAN BOLIAN RICE GENE TECH CO LTD
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