50 results about "Tumour immunotherapy" patented technology

The present invention is in the field of immunotherapy, in particular tumor immunotherapy. The present invention provides pharmaceutical formulations for delivering RNA to antigen presenting cells such as dendrite cells (DCs) in the spleen after systemic administration. In particular, the formulations described herein enable to induce an immune response after systemic administration of antigen-coding RNA.

The invention relates to a genetically modified mesenchymal stem cell (MSC) and medical use thereof in the treatment of tumours, said MSC comprising one or more exogenous nucleic acid molecule(s), wherein said exogenous nucleic acid molecule(s) comprise a region encoding one or more immune response-stimulating or immune response-modulating cytokine(s) operably linked to a promoter or promoter / enhancer combination. The invention encompasses the use of said cells in modulating the tumour microenvironment in order to attract immune effector cells and facilitate their activation and / or adoption of a memory phenotype. One aspect of the invention relates to the use of said cells in anti-tumour treatment comprising the combined administration of said mesenchymal stem cells with anti-tumour immunotherapies, such as checkpoint inhibitors, immune cells, for example T cells, such as T cells with artificial T cell receptors, for example a chimeric antigen receptor (CAR-Ts) or exogenous T-Cell Receptor (TCR) transduced cells, NK cells or macrophages / monocytes, or a cancer vaccine.

OX40 is a potent immune stimulating target. Provided herein is a method of treating cancer, which includes administering to a subject in need of treatment an OX40 agonist and a common gamma chain (yc) cytokine or an active fragment, variant, analog, or derivative thereof. In certain aspects the common gamma chain (yc) cytokine is interleukin-2 (IL-2) or an active fragment, variant, analog, or derivative thereof. Combined treatment with an agonist anti-OX40 mAb and IL-2 synergized to augment tumor immunotherapy against multiple tumor types. Dual therapy was also able to restore the function of anergic tumor-reactive CD8+ T cells.

The invention relates to the field of gene editing and tumor immunotherapy. Specifically, the present invention relates to a method for preparing a modified T cell, such as a CAR-T cell, by gene editing, and the modified T cell prepared by the method and application thereof.

The invention belongs to the technical field of biopharming, and specifically relates to a novel chimeric antigen receptor (CAR) targeting CD19 and applications thereof. The chimeric antigen receptorincludes signal peptide, nanoantibodies targeting CD19, a transmembrane region, costimulatory factors and an intracellular signal domain; and the intracellular signal domain is the mutant CD3[zeta] intracellular signal domain. Through the using of the nanoantibodies targeting CD19 and the mutant CD3[zeta] intracellular signal domain, the molecular weight of the chimeric antigen receptor can be reduced, convenient nucleic acid transfection and molecular biology operation can be achieved, antineoplastic activity can be enhanced, stronger tumor killing effects can be achieved, and therefore, novel approaches can be provided for the development of a novel tumor immunotherapy; and the chimeric antigen receptor can be used in CAR-T therapy, and shows good application prospects.

The invention discloses an application of gold platinum thermometal nano particles on preparation of cell immunotherapy accelerant. According to the application, the hydrogen peroxide enzymatic activity of the gold platinum thermometal nano particles is used, the gold platinum thermometal nano particles can react with hydrogen peroxide in the tumour hypoxia microenvironment and generate oxygen andhave good biocompatibility, the gold platinum thermometal nano particles are combined with cell immunotherapy and used as accelerant of cell immunotherapy, so that the tumour hypoxia situation is improved, inhibition of the tumour hypoxia environment to the killing capacity of the immune cell is lowered, accordingly, the killing effect of the immune cell to the hypoxia tumour cell is remarkably improved, and the gold platinum thermometal nano particles have wide application prospects on tumour immunotherapy.

The invention relates to the field of tumor treatment, particularly to a tumor immunotherapy method for treating tumors and / or preventing tumor recurrence or metastasis through intratumoral administration with a specific cytokine combination, and a corresponding pharmaceutical composition.

The invention provides a fusion protein comprising a flagellin adjuvant and a tumor antigen. Also provided are compositions comprising a flagellin adjuvant and a tumor antigen. The invention also provides pharmaceutical formulations for inducing an immune response against tumor antigen and methods thereof, and a method for treating tumor in tester.

The invention provides a construction method of an immune cell tumor killing capacity detection model. The construction method comprises the following steps: (1) 24 after egg membranes of embryos of zebrafish fertilized for 24-96 hours are removed, injecting tumor cells labeled by a first living cell staining agent into zebrafish bodies, then executing culturing in culture water containing a melanin inhibitor, and screening out the zebrafish with the tail parts labeled by the first living cell staining agent; (2) after the collected zebrafish is anesthetized, the zebrafish is divided into an immune cell injection group and a control group, injecting immune cells labeled by a second living cell staining agent into the immune cell injection group in a microinjection mode, injecting a carriersolution into the control group, and then putting the zebrafish in the culture water containing the melanin inhibitor for continuous culture. Short period, time saving, labor saving and low modelingcost are achieved by adopting the construction method, and a constructed detection model can accurately and objectively evaluate the effect of tumor immunotherapy. The invention also provides a detection model prepared based on the method and application thereof.

The invention relates to an immunologically active peptide-biliverdin conjugate, a preparation method thereof and application thereof in cancer diagnosis, and / or tumor immunotherapy, and / or tumor 'photothermal immunotherapy' (tumor photothermal therapy combined immunotherapy). The conjugate disclosed by the invention not only can stimulate an organism to generate a tumor immune effect, but also can relieve and / or eliminate tumor inflammation, remodel a tumor inflammatory microenvironment and realize photo-thermal immune cancer diagnosis and treatment. The conjugate disclosed by the invention is high in biocompatibility, good in stability and long in half-life period. The conjugate is prepared from immunologically active peptide and biliverdin through chemical synthesis, the peptide end ofthe conjugate plays a role in immunoregulation, and the pigment end of the conjugate plays a role in tumor imaging diagnosis, tumor photo-thermal ablation, immune inflammatory microenvironment regulation and the like. The conjugate can significantly enhance the anti-tumor effect and effectively inhibit tumor metastasis and recurrence.

The invention provides a method for constructing a synovial sarcoma xenograft mouse model with sound immunity. The method comprises the steps of culture of a P0 generation, wherein firstly, fresh tumor tissue masses of a patient with a synovial sarcoma are collected and then transplanted into the body of a mouse with immune deficiency under a sterile condition; a mouse with normal immunity is subjected to ciclosporin pretreatment; culture of Px generations, wherein tumor tissue masses growing in the body of a mouse of any generation from P0-P<x-1> are taken to be inoculated into the body of amouse subjected to the ciclosporin pretreatment to form a Px-generation mouse; culture of Py generations, wherein synovial sarcoma tumor tissue masses growing in the body of a mouse of any generationfrom Px-P<y-1> are taken to be inoculated into the body of the mouse with the normal immunity until tumor tissue grows in the body of the mouse with the normal immunity, and the culture of the Py generations is completed. The method solves the problem that a traditional PDX model has no immune system, provides an in-vivo model for a tumor immunotherapy, and broadens the application range of the PDX model.

The invention relates to the field of genetic editing and tumor immunotherapy, in particular to a method of preparing modified T cells, such as CAR-T cells, through genetic editing, modified T cells prepared via the preparation method, and application of the modified T cells.

A tumor or a malignant disease can be treated by the combined administration of mesenchymal stem cells (MSCs) with an anti-tumor immunotherapy. The MSCs need not be genetically modified, and do not include exogenous nucleic acids that encode immune response-stimulating cytokines. The anti-tumor immunotherapy can be a cellular immunotherapy, such as administration of chimeric antigen receptor (CAR) T cells, in which the T cell receptor binds specifically to a tumor-associated antigen.

The invention provides a gingseng extract product FQR1 as well as a preparation and an application thereof, and provides a FQR1 induced culture method for DC-CIK cell. The method comprises the following steps: a collection of peripheral blood, an acquisition of tumor antigens, a separation of mononuclear cells, a washing, an induction of DC-CIK cells, a culture, etc. The obtained gingseng extract product FQR1 can effectively increase propagation of DC-CIK cells, improve tumor killing activity of DC-CIK cells, and prolong the survival time with tumor of tumor-bearing mice with the DC-CIK cell therapy.

Provided herein are predictive biomarker signatures that identify patients as likely to benefit from TIL therapy. Also provided are tumor immunotherapy resistance pathways that may be targets of combination therapies to enhance TIL therapy.

The invention relates to the field of biotechnologies, and discloses an antitumor composition, a co-expression vector for the same and application of the antitumor composition. The antitumor composition, the co-expression vector and the application have the advantages that combination of 1L-36 gamma and PD-1 (programmed death-1) / PD-L1 (programmed death-ligand 1) signal blocking agents on tumor sites is demonstrated, antitumor immune response in tumor micro-environments can be effectively promoted, and tumor growth can be obviously inhibited; the lifetime of tumor-bearing mice can be greatly prolonged, novel and effective tumor immunotherapy is created, and theoretical and method foundations can be laid for further developing corresponding tumor immunological preparations.

Provided is a novel means for activating an immune response in tumor immunotherapy in which an immune checkpoint inhibitor is used. An immune-checkpoint-inhibitor antitumor effect potentiator having probiotics and prebiotics as active ingredients.

The invention provides a method for constructing an immune healthy synovial sarcoma xenograft mouse model; the method comprises cultivating a generation P0, to be specific, resuscitating a tumor tissue block of a synovial sarcoma patient stored in a liquid nitrogen environment, then transplanting the tumor tissue block into an immunodeficient mouse under a sterile condition, and pretreating a micewith normal immunity by cyclosporine; culturing a generation Px, to be specific, inoculating the tumor tissue block, grown in a mouse from any of the generations P0 to Px, into the mouse pretreated with cyclosporine to form a generation Px mouse; and culturing a generation Py, to be specific, inoculating a synovial sarcoma tumor tissue block, grown in a mouse from any of the generations Px to Py-1, into the mouse with normal immunity until tumor tissues grow in the mouse, so that culturing of the generation Py is completed. The invention solves the problem that the traditional PDX (patient-derived xenograft) model has no immune system, provides an in-vivo model for the research on tumor immunotherapy, and widens the application range of the PDX model.

The invention discloses a serum-free culture medium of CAR-T cells. The serum-free culture medium consists of a basic culture medium and the following components added into the culture medium: nicergoline, icariin, ferulate, IL-2, IL-15, vitamin E and L-glutamine. The nicergoline and the icariin are added into the serum-free culture medium of the CAR-T cells in the invention, so that the proliferation activity of the CAR-T cells can be effectively improved; and a large number of CAR-T cells can be harvested within a short time. The ferulate is also added, so that the number of living cells in the harvested cells is easily increased; and the cell survival rate is increased. The components, the L-2, the IL-15, the vitamin E and the L-glutamine jointly act; the proliferation activity of the CAR-T cells is improved; the used components are safe; blood components such as plasma are not added; the culture cost of the CAR-T cells is reduced; and the burden of a patient is relieved. The invention further provides a culture method of the CAR-T cells; the culture method is simple and convenient to operate and easy to implement; and popularization and application of the CAR-T cells in the field of tumour immunotherapy are facilitated.

The present invention relates to tumor immunotherapy. In particular, the present invention provides methods and compositions for converting cancer cells into antigen presenting cells. Thus the present invention provides immunogenic compositions for the treatment and prevention of cancer.

The invention discloses an intestinal flora regulator. The intestinal flora regulator comprises icarisid I. The intestinal flora regulator can regulate the structure of intestinal flora, improve the metabolism of the flora, convert the intestinal flora of a patient into a healthy intestinal flora outline, enhance the immunity of a host and resist and inhibit cancers, and can be applied to the fields of medicines, foods and the like. The invention further discloses an application of the intestinal flora regulator to increment of the content of intestinal probiotics and the content of intestinalflora secondary metabolites, and an application of the intestinal flora regulator to preparation of functional foods, anti-cancer drugs or cancer immunotherapy. The content of lactobacillus and bifidobacterium in intestinal tracts can be increased, and generation of metabolite short-chain fatty acid is promoted; the intestinal flora regulator is free from toxic or side effects, can be used for daily eating, regulates intestinal flora and improves immunity; the icariside I has a good inhibition effect on tumor cells, and can also improve the low reaction rate of a patient to tumor immunotherapy and possible side effects; and a new direction is provided for the application of the icariside I.

The invention discloses a preparation method of lipidosome containing alkannin and anthracycline chemotherapeutic drugs and an application of the lipidosome in synergistic anti-tumor immunotherapy, and belongs to the technical field of pharmaceutical preparations. Firstly, alkannin and an anthracycline drug mitoxantrone or adriamycin are simultaneously encapsulated in a liposome, a synergistic proportion is screened by using a co-loaded liposome through a cytotoxicity experiment, an uptake experiment and ICD induction capability, and the prepared dual-drug-loading liposome has a pH / GSH dual-response release characteristic, can deliver a drug to a tumor tissue, the anti-tumor effects is synergistically induced, the administration dosage of the two drugs is reduced, the toxic and side effects of the drugs are reduced, and the purposes of enhancing the effect and reducing the toxicity are achieved.

The success of anti-tumor immune responses requires effector T cells to infiltrate solid tumors, a process guided by chemokines. Herein, we demonstrate that in vivo post-translational processing of chemokines by dipeptidylpeptidase 4 (DPP4, also known as CD26) limits lymphocyte migration to sites of inflammation and tumors. Inhibition of DPP4 enzymatic activity enhanced tumor rejection by preserving biologically active CXCL10, and increasing trafficking into the tumor by lymphocytes expressing the counter-receptor CXCR3. Furthermore, DPP4 inhibition improved adjuvant-based immunotherapy, adoptive T cell transfer and checkpoint blockade. These findings provide the first direct in vivo evidence for controlling lymphocyte trafficking through CXCL10 cleavage and support the use of DPP4 inhibitors for stabilizing the biologically active form of chemokines as a strategy to enhance tumor immunotherapy.

The invention provides a method for constructing an immune-sound synovial sarcoma xenograft mouse model, which includes the cultivation of the P0 generation: first resuscitating the tumor tissue block of a synovial sarcoma patient stored in a liquid nitrogen environment, and then under aseptic conditions It was transplanted into immunodeficient mice; normal immune mice were pretreated with cyclosporine; culture of Px generation: the tumor tissue pieces grown in any generation of mice from P0 to Px‑1 generation were inoculated into the above-mentioned From the mice pretreated with cyclosporine, the Px generation mice were formed; the cultivation of the Py generation: take the synovial sarcoma tumor tissue pieces grown in any generation of mice from the Px generation to the Py-1 generation above and inoculate them into normal immune cells. In the mouse body, until the tumor tissue grows in the body, the culture of the Py generation is completed. The invention improves the problem that the traditional PDX model has no immune system, provides an in vivo model for the research of tumor immunotherapy, and broadens the application range of the PDX model.

The invention discloses a cytoplasmic polyadenylation element-binding protein 4 vaccine, and preparation and application thereof. The vaccine is a ISCOM-DNA compound, the preparation method of the vaccine comprises: cloning of full-length DNA of cytoplasmic polyadenylation element-binding protein 4, cloning of full-length DNA of tumor necrosis factor BAFF, construction of a recombinant vector pStar-CPEB4 / BAFF, construction of a pStar-CPEB4 / BAFF engineering bacteria, inducible expression of CPEB4, and preparation of the ISCOM-DAN compound. The vaccine has good application prospect on neuroglioma controlling. The vaccine has the advantages of being efficient, low in toxicity, simple in preparation method and the like, and is applicable to medicines for tumour immunotherapy.

The invention discloses an immune checkpoint inhibitor five-membered heterocycle substituted biphenyl compound capable of blocking a PD-1 / PD-L1 signal pathway and a preparation method and application thereof. The compound is shown in the formula I as described in the specification. The compound is novel in structure and can be orally taken, the defects of treatment and drug resistance of a monoclonal antibody immune checkpoint inhibitor are overcome, and as a small-molecule inhibitor, the preparation is simple, and industrial production is convenient; various related tumor diseases can be treated through tumor immunotherapy by regulating and controlling a PD-1 / PD-L1 signal pathway, and potential patent medicine prospects are achieved.

The invention belongs to the fields of tumor immunotherapy and molecule immunology, and particularly relates to a humanized antihuman CTLA4 monoclonal antibody. The invention further relates to a preparation method and application of the humanized antihuman CTLA4 monoclonal antibody. Particularly, the humanized antihuman CTLA4 monoclonal antibody provided by the invention has high substantivity and high specificity for CTLA4, can stimulate T cells to secrete cytokine, for example, immunization negative regulation of the CTLA4 can be specially removed, and T cell cytokine can be activated. Thefunctional humanized antihuman CTLA4 monoclonal antibody provided by the invention can block a CTLA4 signal route to activate the T cells, and further the purpose of tumor immunotherapy is realized.

Provided is a tumor antigen protein, gene, or peptides derived from topoisomerase 2 alpha. As it is discovered that topoisomerase 2 alpha binds to an MHC class I or II antigen, thereby forming a complex, and the complex is recognized by cytotoxic T lymphocytes, the tumor antigen can be used in tumor immunotherapy.