Adventitious bud inducing and rooting medium for radicle of Carya cathayensis Sarg and tissue culture method

Abstract

The invention discloses an adventitious bud inducing and rooting medium for radicle of Carya cathayensis Sarg, which comprises four culture media for different induction periods: (1) a primary culture medium, (2) a radicle adventitious bud induction culture medium, (3) an adventitious bud enrichment culture medium, (4) an adventitious bud rooting medium. The four culture media all takes MS as a basic culture medium and are added with sucrose, agar A and other substances and have a pH value of 5.7. A tissue culture method for adventitious bud inducing and rooting of the radicle of the Carya cathayensis Sarg with the four culture media comprises the following five steps: 1, collecting and disinfecting explants; 2, performing primary culture; 3, inducing the adventitious bud of the radicle; 4, performing adventitious bud enrichment culture; and 5, performing rooting induction. The culture of the five steps lays a solid foundation for rapidly culturing saplings of the Carya cathayensis Sarg with good quality. When the medium is used for tissue culture, regenerated plantlet can maintain the excellent properties of parents; the propagation speed of the saplings is improved; the propagation number is expanded; seeds are effectively utilized; and the seedling period is shortened so as to contribute to meeting social requirements.

Description

technical field [0001] The invention relates to a culture medium and a tissue culture method for taking hickory nut young embryos as explants, developing to form radicles, then forming adventitious buds, and inducing rooting. Background technique [0002] Hickory nut (Carya cathayensis Sarg.) is an excellent economic tree species of fruit and wood of the Juglandaceae Hickory genus. In the natural environment, Hickory nut is propagated by seeds. Dried pecans can be processed into various special flavor foods and health products. The oil content of pecans is as high as 70% to 74%, rich in unsaturated fatty acids, 7.8% to 9.6% of crude protein and K, Ca, Mg, Na, etc. Mineral elements necessary for the human body. Hickory has hard wood, beautiful texture and corrosion resistance. It is widely used in military industry, shipbuilding and construction industry. It is a very good economic tree species in our country. However, the distribution area of ​​hickory tree is narrow, and ...

AI Technical Summary

Problems solved by technology

[0004] The economic value of hickory tree species is more and more recognized by people, and the enthusiasm for developing hickory nut production is surging spontaneously. Relying on traditional seed propagation and grafting propagation, it is impossible to meet the actual needs in terms of quality and quantity. For this reason, the present invention The technical problem to be solved is to provide a culture medium and tissue culture method for inducing radicle development, formation of adventitious buds and rooting of adventitious buds by taking the immature embryos of hickory nuts as explants