Adventitious bud inducing and rooting medium for radicle of Carya cathayensis Sarg and tissue culture method

A technology for rooting medium and medium, applied in the fields of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve problems such as the insufficiency of quality and quantity

Inactive Publication Date: 2010-07-28
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The economic value of hickory tree species is more and more recognized by people, and the enthusiasm for developing hickory nut production is surging spontaneously. Relying on traditional seed propagation and grafting propagation, it is impossible to meet the actual needs in terms of quality a

Method used

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Examples

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Embodiment Construction

[0019] The present invention is described in further detail below in conjunction with the examples: in the culture medium for the induction and rooting of the hickory radicle adventitious buds, the basic medium for selection is MS medium, which is through using four basic mediums of MS, 1 / 2MS, WPM, and DKW. Sucrose 20-30g / L, agar A 6.5-8.5g / L, Picloram 4-amino-3,5,6-trichloropyridine carboxylic acid 0.01mg / L, 6-BA 6-amino Adenine 1.0-3.0mg / L, pH value 5.7, the experiment was carried out, each group was repeated 3 times, and each basic medium had 20 explants in the comparative experiment. After treatment in MS medium for 30 days, the radicle develops the best, the number of induced adventitious buds is the largest, and the quality is the best.

[0020] In a word, the culture medium and its proportioning value disclosed by the present invention, temperature, coagulant etc. can also be replaced by such as glucose, crystal agar, etc., and the proportioning value of each raw materi...

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PUM

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Abstract

The invention discloses an adventitious bud inducing and rooting medium for radicle of Carya cathayensis Sarg, which comprises four culture media for different induction periods: (1) a primary culture medium, (2) a radicle adventitious bud induction culture medium, (3) an adventitious bud enrichment culture medium, (4) an adventitious bud rooting medium. The four culture media all takes MS as a basic culture medium and are added with sucrose, agar A and other substances and have a pH value of 5.7. A tissue culture method for adventitious bud inducing and rooting of the radicle of the Carya cathayensis Sarg with the four culture media comprises the following five steps: 1, collecting and disinfecting explants; 2, performing primary culture; 3, inducing the adventitious bud of the radicle; 4, performing adventitious bud enrichment culture; and 5, performing rooting induction. The culture of the five steps lays a solid foundation for rapidly culturing saplings of the Carya cathayensis Sarg with good quality. When the medium is used for tissue culture, regenerated plantlet can maintain the excellent properties of parents; the propagation speed of the saplings is improved; the propagation number is expanded; seeds are effectively utilized; and the seedling period is shortened so as to contribute to meeting social requirements.

Description

technical field [0001] The invention relates to a culture medium and a tissue culture method for taking hickory nut young embryos as explants, developing to form radicles, then forming adventitious buds, and inducing rooting. Background technique [0002] Hickory nut (Carya cathayensis Sarg.) is an excellent economic tree species of fruit and wood of the Juglandaceae Hickory genus. In the natural environment, Hickory nut is propagated by seeds. Dried pecans can be processed into various special flavor foods and health products. The oil content of pecans is as high as 70% to 74%, rich in unsaturated fatty acids, 7.8% to 9.6% of crude protein and K, Ca, Mg, Na, etc. Mineral elements necessary for the human body. Hickory has hard wood, beautiful texture and corrosion resistance. It is widely used in military industry, shipbuilding and construction industry. It is a very good economic tree species in our country. However, the distribution area of ​​hickory tree is narrow, and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张启香黄坚钦王正加黄有军胡恒康
Owner ZHEJIANG FORESTRY UNIVERSITY
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