Method for mutagenizing barbadosnut seeds by ethylmethane sulfonate

A technology of ethyl methyl sulfonate and Jatropha curcas, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of immature technology, lack of accuracy in optimal processing time and concentration, and heavy workload and other problems, so as to avoid the large variation of germination rate, promote the development of industrialization, and reduce the time and concentration.

Inactive Publication Date: 2013-10-09
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Jatropha curcas is an oil plant, and its seeds have high oil content, so there are many factors that affect the germination rate of the seeds. However, the optimal treatment time and concentration proposed after the seeds are soaked and treated by EMS mutagenes...

Method used

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  • Method for mutagenizing barbadosnut seeds by ethylmethane sulfonate
  • Method for mutagenizing barbadosnut seeds by ethylmethane sulfonate
  • Method for mutagenizing barbadosnut seeds by ethylmethane sulfonate

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Embodiment 1

[0022] Embodiment 1 The first preferred embodiment of the method of a kind of ethyl methanesulfonate mutagenesis treatment of Jatropha curcas seeds of the present invention

[0023] This embodiment is a preferred embodiment of a method for mutagenic treatment of Jatropha curcas seeds with ethyl methanesulfonate of the present invention. The present embodiment adopts the improved method of ethyl methanesulfonate mutagenesis Jatropha curcas seed, comprises the following specific steps:

[0024] (1) Seed pre-germination: select the plump Jatropha curcas seeds of the current season, put the seeds in distilled water, soak them at room temperature for 24 hours, pick them up and drain them, and cultivate them at 30°C for 7 days. In another embodiment , cultivate and accelerate germination for 5 days; during this period, quickly rinse the seeds with distilled water and drain them every 2 days, and then culture and accelerate germination at 30°C;

[0025] (2) Seed mutagenesis treatmen...

Embodiment 2

[0028] Embodiment 2 The second preferred embodiment of the method of a kind of ethyl methanesulfonate mutagenesis treatment of Jatropha curcas seeds of the present invention

[0029] The second preferred embodiment of the method for the mutagenic treatment of Jatropha curcas seeds of a kind of ethyl methanesulfonate of the present invention, comprises the following steps:

[0030] (1) Pre-germination of seeds: select plump Jatropha seeds and soak them in water;

[0031] (2) Seed mutagenesis treatment: select the seeds that have been pre-germinated in step (1) and soaked in ethyl methanesulfonate solution;

[0032] (3) Pregermination of induced seeds: soaking the seeds obtained in step (2) in clean water to obtain germinated seeds;

[0033] (4) Seedling cultivation: plant the germinated seeds obtained in step (3) in a planting tray for seedling cultivation.

Embodiment 3

[0034] Embodiment 3 The third preferred embodiment of the method of a kind of ethyl methanesulfonate mutagenesis treatment of Jatropha curcas seeds of the present invention

[0035] A preferred embodiment of the method for the mutagenic treatment of Jatropha curcas seeds of a kind of ethyl methanesulfonate of the present invention, comprises the following steps:

[0036] (1) Pre-germination of seeds: select the plump Jatropha curcas seeds of the current season, put the seeds in distilled water, soak them at room temperature for 24 hours, pick them up and drain them, and cultivate them at 30°C for 7 days; During this period, the seeds were quickly rinsed with distilled water and drained every 2 days, and then cultured at 30°C to accelerate germination.

[0037] (2) Seed mutagenesis treatment: select the seeds obtained in step (1) that have undergone preliminary germination and have broken shells and exposed white seeds with unprotruded radicles, soaked in ethyl methanesulfonate...

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Abstract

The invention discloses a method for mutagenizing barbadosnut seeds by ethylmethane sulfonate. The method disclosed by the invention comprises the following steps of: first, performing pre-bud forcing treatment at 30 DEG C on the barbadosnut seeds, selecting the seeds which are broken and exposed with unexpanded radicles after 3-7 days and soaking the seeds in a 0.9% EMS (Ethylmethane Sulfonate) liquor and soaking for 7 hours; forcing buds of the treated seeds at 28 DEG C and culturing for 3-4 days; and sowing mutagenized survival seeds in a soft peat soil substrate with radicles facing downward, and earthing by 1cm to breed. According to the method, the defects in the prior art are overcome, the mutagenizing concentration and time of EMS are reduced and the mutagenizing efficiency of the barbadosnut seeds by ethylmethane sulfonate is improved so as to facilitate selection by mutation of barbadosnut and promote industrialized development of the barbadosnut.

Description

technical field [0001] The invention relates to the technical field of phytochemical mutation breeding, in particular to a method for mutagenic treatment of Jatropha curcas seeds with ethyl methanesulfonate (EMS). Background technique [0002] The energy plant Jatropha curcas (Jatrophacurcas L.), also known as Jatropha curcas, Jatropha curcas, etc., is a deciduous small tree or shrub of the Euphorbiaceae Jatropha genus. It is drought-tolerant and thin-barren, and is mainly distributed in tropical and subtropical regions. . Jatropha curcas is one of the plants with high seed oil content, and is internationally recognized as an ideal raw material for biodiesel preparation, and has high comprehensive utilization value. Jatropha curcas has been listed as a key development target of biodiesel because of its advantages of "not competing with food for land and people for food". Although the Jatropha curcas biodiesel industry has shown a trend of rapid development, it will take a ...

Claims

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Application Information

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IPC IPC(8): A01H1/06
Inventor 张剑亮王继华王丽曹干
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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