Total flavone extract of lonicera macranthoides leaves, and preparation method and application thereof

The technology of honeysuckle and total flavonoids is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, which can solve the problems of increasing production cost, low product purity, polluting the environment, etc., and achieves good activity, simple craftsmanship

Inactive Publication Date: 2013-03-27
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: 1. small molecular impurities such as inorganic salts and amino acids cannot be effectively removed, and the product quality is poor; 2. ethanol organic solvents cannot be further removed, the purification is not thorough, and "three wastes discharge" pollutes the environment; 3. the purity of the product is not high. The color is not good, etc.
The main shortcoming of this method is: 1. use ethanol solution as extraction liquid, the solution impurity after extraction is many, is unfavorable for follow-up purification, directly causes the purity of product low and the quality color and luster of product; 2. the used temperature of extraction is too high, to The quality of the product is affected, the requirements for the production equipment are high, the production cost is increased, the waste of energy is not environmentally friendly; ③ the extracted solution has not been further purified, and the product quality is low

Method used

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  • Total flavone extract of lonicera macranthoides leaves, and preparation method and application thereof
  • Total flavone extract of lonicera macranthoides leaves, and preparation method and application thereof
  • Total flavone extract of lonicera macranthoides leaves, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of total flavonoids extract in embodiment 1 gray felted honeysuckle leaves

[0021] Weigh 10 kg of honeysuckle leaves, dry and pulverize, reflux extract with a weight ratio of medicinal materials and purified water of 1:3 for 30-50 minutes, vacuum filter the extract, collect the filtrate for later use, add medicinal materials after precipitation and extract again;

[0022] Concentrate the filtrate collected above in a water bath to 1 / 3 of the original volume, and collect the concentrate I;

[0023] Add 95% ethanol to the concentrated solution Ⅰ until the concentration of ethanol in the concentrated solution is 60%, let it stand for 24 hours, then centrifuge at 3000 rpm for 20 minutes, take the supernatant and perform the same method for the second time Alcohol precipitation and centrifugation, collect the supernatant of the second centrifugation, recover ethanol until there is no alcohol smell, and obtain the concentrated solution II;

[0024] Dissolve ...

Embodiment 2

[0029] Example 2 Protective effect of total flavonoids extract from leaves of Lonicerae argentina on immune liver injury mice

[0030] 70 KM mice, male, weighing 18-22g, were randomly divided into normal group, model group, low-dose group (100mg / kg), medium-dose group (200mg / kg), High-dose group (400mg / kg) group, extraction control group (400mg / kg) and bifendate 150mg / kg, 10 mice in each group, except the normal group on the first day of modeling, the tail of each mouse in the other groups Intravenous injection of Bacillus Calmette-Guerin (BCG) 12.5mg / mL (about 5×10 7 number of viable bacteria), each treatment group was given the corresponding drug ig; the normal group and the model group were given the same volume of vehicle ig for 10 consecutive days. On the 10th day, except for the normal group given an equal volume of vehicle IV, each mouse in the other groups was intravenously injected with bacterial lipopolysaccharide (LPS) 37.5 μg / mL. After fasting for 16 hours after ...

Embodiment 3

[0039] The effect of total flavonoids extract in the leaf of embodiment 3 gray felted honeysuckle on the blood sugar, blood fat of high fat + high sugar + streptozotocin diabetic model rats

[0040] Ten of the 70 rats were left without any treatment, and they were regarded as the normal group; the remaining 70 rats were continuously fed with high-fat and high-sugar diet for one month, and after fasting for 24 hours, they were intraperitoneally injected with streptozotocin (STZ) 25mg / kg. Blood glucose was measured after 72 hours, and the rats with blood glucose value ≥ 11.0mmol / L were randomly divided into model group, low-dose group (100mg / kg) of total flavonoids extract of Lonicerae Lonicerae Lonicerae, and middle-dose group (200mg / kg) , high-dose group (400mg / kg) group, extraction control group (400mg / kg) and metformin 800mg / kg, 10 rats in each group, administered once a day for one month in a row. Blood glucose, total cholesterol (TC), and triglyceride (TG) were detected ...

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Abstract

The invention provides an extraction method and application of total flavone extract from lonicera macranthoides leaves. The total flavone of lonicera macranthoides leaves is furthest extracted by adopting a water-extraction and alcohol precipitation process; and the flavone compound is selectively separated through macroporous resin, and is concentrated and dried to obtain the flavone compound with high purity. The process is simple, and is suitable for large-scale industrial production, and the total flavone content of the total flavone extract prepared from the lonicera macranthoides leaves is higher than 50 percent. The total flavone extract has excellent activity in treating autoimmune hepatitis, hyperglycemia and hyperlipidemia syndromes.

Description

technical field [0001] The invention relates to a method for extracting active components of traditional Chinese medicinal materials, in particular to a total flavonoid extract of Lonicerae argentina leaves, a preparation method and application thereof. technical background [0002] Lonicera cinerea is a plant of Lonicera genus Lonicera. It has the effect of clearing away heat and detoxifying, cooling and dispersing wind-heat. Lonicerae cinerea is not only a commonly used traditional Chinese medicine, but also a green natural product with the same origin of medicine and food. However, its by-product, the leaf, has been regarded as a non-medicinal part and has not been well utilized for a long time and was discarded. . The existing literature shows that the content of flavonoids in the old leaves of Lonicera cinerea is about 2.78 times that of medicinal honeysuckle and 6.98 times that of Lonicera vine. The leaves of Lonicerae argentina may be a better medicinal part, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/355A61P37/02A61P1/16A61P3/10A61P3/06A61K127/00
Inventor 李卿李隆云秦剑罗先钦周兴胡荣
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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