Breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1

Abstract

The invention discloses a breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1, comprising the following steps of: taking lamina extracted in the current year as explant, sterilizing the lamina and inoculating the lamina on an induced medium to culture callus in an inducing way, transplanting the callus into a differential medium to carry out the differential cultivation of adventitious bud, cutting the obtained primary cluster buds into single buds and transplanting the single buds into a subculture medium to carry out the subculture of adventitious buds, when the sub-cultured cluster buds grow to the height of 1.5-2cm, cutting the sub-cultured cluster buds into single buds and transplanting the single buds into a rooting medium to culture in a rooting way, andtransplanting generated test-tube plantlet into a pearlite-peat soil mixed matrix with the volume ratio of 1:9 to culture, to obtain the tissue culture seedling of lonicera macranthoides Yulei No.1. According to the method, the induced medium, the differential medium, the subculture medium and the rooting medium can be optimized. The breeding method has the advantages of being high in callus induction rate, high in adventitious bud differentiation rate and multiplication coefficient, high in rooting rate, high in test-tube plantlet transplanting survival rate, robust in sprout growth, normal in lamina shape and color, subcutaneous in rooting, quick in the germination of new leaves and the like, so that the requirement of the large-area and large-scale cultivation of the lonicera macranthoides Yulei No.1 can be met.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a breeding method of plant tissue culture seedlings. Background technique [0002] Gray felted honeysuckle (Lonicera m acranthoides Hand. Mazz) is a perennial semi-evergreen winding small shrub or erect small shrub plant of Lonicera genus Lonicera. The content of chlorogenic acid in its flower buds is the highest among all kinds of honeysuckle in the country. One of the main varieties of commercial honeysuckle. [0003] Yulei No. 1 was approved by the Chongqing Forest Variety Approval Committee in 2008 as a new variety of Lonicera aureus. The flower yield of this variety is more than 400 kg per mu, which is 30-80% higher than that of the traditional planting of Lonicera chinensis. Its stress resistance and adaptability are also stronger than that of traditional home-grown Lonicera spp. ~30 days and the entire flowering period is full of flower buds, basically no flow...

AI Technical Summary

Problems solved by technology

Due to the large variation in plant traits of this variety, clonal breeding methods such as cuttings and grafting are usually used to propagate excellent single-plant clones. However, there are not many rootstocks and scion resources for grafting, and the survival rate of cuttings is lower than 40%, resulting in a low reproductive coefficient. , cannot meet production and market demand

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