Triploid lonicera confusa and cultivation method thereof

A cultivation method and triploid technology, applied in the fields of botany equipment and method, application, plant gene improvement, etc., can solve the problem of no public reports on the cultivation method of triploid mountain silver flower, low planting economic benefit, green field Low acid content and other problems, to achieve the effect of solving the hybrid flowering period, shortening the breeding time, and high content of active ingredients

Active Publication Date: 2012-03-28
桂林亦元生现代生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the flower buds of the conventional mountain silver flower are short, the flower bud period is short, easy to open, the flower wall is thin, and it is inconvenient to pick.
[0003] Predecessors have selected and bred varieties with early flowering, long flower buds, high chlorogenic acid content, high and stable yields from conventional Lime japonicus plants (chromosomal karyotype index is 2n=18), but the flower bud period is short, easy to open, and easy to pick. It is inconvenient, and the appearance is poor after being made into dry products. For example, the red glandular honeysuckle in Mashan County, Guangxi, enters the green bud stage on April 1 of each year, and it only takes 10 days from the green bud stage to the silver flower stage; the corolla is also bred. Varieties that have never cracked and have a long flowering period, but have thin flower walls, low chlorogenic acid content, and come on the market late, such as the gray felted honeysuckle in Guangxi Ziyuan County, which only enters the green bud period on June 20 every year
Predecessors have carried out polyploid induction on honeysuckle plants of the same family and genus, and the bred autotetraploid plants (chromosomal karyotype index is 4n=36) have thick branches and leaves, increased number of flower buds, thickened flower walls, and chlorogenic acid. The higher the content, the higher the yield; but the bud period is short, easy to open, in opposite inflorescences, and it is inconvenient to pick
So far, there has been no public report on the triploid Silverflower (chromosomal karyotype index is 3n=27) and its breeding method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) The adult plant of Lonicera red glandularis produced in Mashan County, Guangxi, which has the characteristics of early flowering, long flower buds, high content of active ingredients, cluster-like inflorescence, high and stable yield (abbreviated as plant 1), produced in Ziyuan County, Guangxi The adult plants (plant 2 for short) of Lonicerae argentina with the characteristics of long flowering period, no cracking of the corolla, high content of active ingredients, cluster-like inflorescence, high and stable yield (abbreviated as plant 2) were planted according to conventional techniques;

[0023] 2) When the flower buds of plant 1 have just entered the green bud stage, wrap the flower buds with absorbent cotton respectively, drop colchicine with a mass concentration of 0.5% into the absorbent cotton, drop 5 times a day, drop 2mL for the first time, and drop 2mL for the rest every day. Drop 1mL each time, continuously drop for 7 days, and then remove the wrapping mate...

Embodiment 2

[0030] 1) Plant A and plant B according to conventional techniques respectively;

[0031] 2) When the flower buds of plant A have just entered the green bud stage, wrap the flower buds with absorbent cotton respectively, drop pentoxychlor with a molar concentration of 280 μmol / L into the absorbent cotton, drop 3 times a day, 1 mL each time, continuously Drop for 7 days, then remove the wrapping material;

[0032] 3) Pick the flowers induced by plant A and in the white bud stage, take out the anthers and spread them on clean white paper (no more than 10 anthers per square centimeter), place them in a desiccator equipped with anhydrous magnesium sulfate, and Seal and dry for 40 hours at room temperature, collect dried pollen, determine the particle size of diploid large pollen by detecting the chromosome karyotype index of pollen with different particle sizes, and then screen out diploid large pollen according to conventional techniques, and store at -2°C Save the backup in the...

Embodiment 3

[0038]1) The adult plant of Lonicera red glandularis produced in Mashan County, Guangxi, which has the characteristics of early flowering, long flower buds, high content of active ingredients, cluster-like inflorescence, high and stable yield (abbreviated as plant 1), produced in Ziyuan County, Guangxi The adult plants (plant 2 for short) of Lonicerae argentina with the characteristics of long flowering period, no cracking of the corolla, high content of active ingredients, cluster-like inflorescence, high and stable yield (abbreviated as plant 2) were planted according to conventional techniques;

[0039] 2) When the flower buds of plant 2 have just entered the green bud stage, use a micro-syringe to inject a pentoxychlor solution with a molar concentration of 150 μmol / L into the base of the flower buds along the longitudinal axis of the flower buds, and inject 50 μL each time twice a day, continuously Injection for 3 days;

[0040] 3) Pick the induced flowers of plant 2 that...

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PUM

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Abstract

The invention discloses triploid lonicera confusa and a cultivation method thereof. The cultivation method comprises the following steps: taking grown-up plants of diploid lonicera hypoglauca miq and lonicera macranthoides as original parents, planting the parents according to the conventional technology, and then mutually pollinating corresponding plants in a hybridization manner; inducing the parents to form diploid pollen when the parents just enter a green bud period; picking the induced flower in a white bud period, taking out and drying anther, and then screening the diploid pollen for low temperature storage; hybridizing the diploid pollen with haploid oocytes of corresponding plants to obtain hybrid seeds; breeding the hybrid seeds into complete plants and then screening triploid plants; and planting the triploid plant according to the conventional technology, and budding to obtain triploid lonicera confusa. Compared with the prior art, by adopting the method, the diploid pollen can be screened more quickly, the difficulty of sterility in a hybridization period can be solved, the breeding time can be obviously shortened, the operation is simple and feasible, the genetic advantages of the triploid lonicera confusa are higher than those of diploid or tetraploid plants, and the planting economic benefit is high.

Description

technical field [0001] The invention relates to the technical field of biological breeding, in particular to a triploid mountain silver flower and a cultivation method thereof. Background technique [0002] Mountain silver flower is the dry flower bud or the initial stage of Lonicera macranthoides Hand.-Mazz., Lonicera hypoglauca Miq., Lonicera confuseDC., or Lonicera fulvotomentosa Hsu et S.C.Cheng. The flowers that bloom are recorded in the 2010 edition of "Chinese Pharmacopoeia". Its main components are chlorogenic acid, Lonicerae saponin B, and Dipsacus saponin B. effect. Compared with the same plant, honeysuckle, its flower buds are hairless and widely applicable; with high content of chlorogenic acid, it is the first choice raw material for extracting chlorogenic acid monomer; it was launched early, coincides with the season for the pharmaceutical and food industries, and seized market opportunities . Therefore, mountain silver flower is widely used in medicine, foo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01H1/02A01H1/04
Inventor 蒋向军杨洪元覃吉胜蒋剑刚羽运飞覃慧慧
Owner 桂林亦元生现代生物技术有限公司
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