A kind of preparation method of rapid separation of white fungus pure mycelium and white fungus female species

A technology for white fungus and female species, which is applied in the fields of botanical equipment and methods, horticulture, agriculture, etc., can solve the problems of easy yellowing, weakened vitality, unfavorable for long-term preservation, etc., and achieves robust growth, simple and convenient operation, and shortened preparation time. Effect

Active Publication Date: 2021-09-28
GUANGZHOU GENSOURCE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tremella pure mycelium is stored on agar medium, which tends to turn yellow, weaken its vitality, easily gelatinize to form small ear pieces, or transform into yeast-like conidia, which is not conducive to long-term storage

Method used

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  • A kind of preparation method of rapid separation of white fungus pure mycelium and white fungus female species
  • A kind of preparation method of rapid separation of white fungus pure mycelium and white fungus female species
  • A kind of preparation method of rapid separation of white fungus pure mycelium and white fungus female species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The present embodiment is a preparation method for rapidly separating pure mycelium of Tremella fungus and female Tremella species, and its specific preparation steps are as follows:

[0025] (1) Separation of fragrant ash mycelium: On the fungus bag for cultivating white fungus, near the part with marbled markings at the bottom, dig out a small piece of sawdust, and insert it into a test tube of potato dextrose agar medium. This part contains a lot of mycelium of fragrant ash. After inoculation, it is placed in the environment of 23-25 ​​℃. After 3-5 days, white and thick mycelium will grow, and then it will turn yellow-green and then black. Pick the black part with an inoculating needle and put it into a new test tube. This transfer several times, that is, pure incense ash mycelium.

[0026] (2) Preparation of the wood chips culture medium containing the ash ash fungus culture: inoculate the isolated and purified ash ash mycelium on the sterilized bottled wood chips ...

Embodiment 2

[0030] The present embodiment is the growth situation of white fungus pure mycelium on agar medium ( image 3 ).

[0031]The white fungus fruiting body that meets the standard of the species of ear in Example 1 is used as the separation material, and a small ear piece of about 1 square centimeter is cut, rinsed 2-3 times with sterile water, and placed in 75% alcohol or 0.1% mercuric chloride. Soak in the solution for two minutes to kill the bacteria on the surface of the ear piece. Immediately put the small ear pieces soaked in the bactericide into sterile water, rinse 2-3 times, and then dry the water with sterile gauze, and inoculate it into the sterilized agar medium.

Embodiment 3

[0033] This example shows the growth of pure mycelium of Tremella on the wood chip medium.

[0034] The white fungus fruiting body that meets the standard of the species of ear in Example 1 is used as the separation material, and a small ear piece of about 1 square centimeter is cut, rinsed 2-3 times with sterile water, and placed in 75% alcohol or 0.1% mercuric chloride. Soak in the solution for two minutes to kill the bacteria on the surface of the ear piece. Immediately put the small ear pieces soaked in the fungicide into sterile water, rinse 2-3 times, and then absorb the water with sterile gauze, and inoculate it into the sterilized sawdust medium.

[0035] The results of Examples 1, 2, and 3 are compared as follows:

[0036] Growth of pure mycelium of Tremella on different media

[0037]

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PUM

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Abstract

The invention discloses a preparation method of white fungus female species. The present application prepares a wood chip culture medium containing a gray fungus culture by isolating the fungus ash mycelia, and then cross-cultures the isolated white fungus pure mycelia and the white fungus mycelia on the wood chip culture medium containing a gray fungus culture to prepare tremella female species. Compared with the traditional method, the white fungus pure mycelium separated by the present invention has the characteristics of short germination time, strong growth, white and dense, etc.; the separation of white fungus pure mycelia and the preparation of white fungus female species are all completed in the same bottle of sawdust culture medium. The operation is simple and convenient, which avoids damage to the pure mycelium of white fungus by multiple transfers and possible bacterial contamination during the transfer process, shortens the preparation time of the white fungus mother seed, and effectively reduces the preparation cost of the white fungus mother seed.

Description

technical field [0001] The invention relates to the technical field of edible fungus culture, in particular to a preparation method of Tremella female species. Background technique [0002] Tremella is the fruiting body of Basidiomycota, Tremella, Tremella, Tremella, Tremella, also known as white fungus, snow fungus, white fungus, etc., and has the reputation of "the crown of fungi". Tremella is generally chrysanthemum-shaped or cockscomb, soft and white, translucent and elastic. Tremella, as a traditional edible fungus in my country, has always been a favorite food of the majority of people. It is usually used as the main ingredient for cooking various dishes, and the active ingredient Tremella polysaccharide contained in it has special health care function. The biggest difference from common edible fungus strains is that Tremella strains have two kinds of strains, that is, regardless of the preparation of parent species, original species or cultivated species, each test ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 涂桂洪邓惠平谭志康潘忠林陈品健
Owner GUANGZHOU GENSOURCE BIO TECH CO LTD
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