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Rooting culture medium and rooting culture method for tissue culture seedling of lonicera macranthoides Hand.Mazz Yulei #1

A rooting medium and a technology for rooting culture, applied in the field of plant tissue culture, can solve the problems of restricting industrial production, unable to meet a large number of demands, difficult rooting in tissue culture of honeysuckle, and achieving the effect of improving the rooting rate

Inactive Publication Date: 2014-02-05
CHONGQING UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to take root in tissue culture of Lonicera argentina, which restricts its industrial production and cannot meet the large demand for Lonicera argentina in today's society.

Method used

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  • Rooting culture medium and rooting culture method for tissue culture seedling of lonicera macranthoides Hand.Mazz Yulei #1
  • Rooting culture medium and rooting culture method for tissue culture seedling of lonicera macranthoides Hand.Mazz Yulei #1
  • Rooting culture medium and rooting culture method for tissue culture seedling of lonicera macranthoides Hand.Mazz Yulei #1

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Embodiment Construction

[0017] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0018] The test material Lonicera tomentosa Yulei No. 1 tissue culture seedling used in the embodiment of the present invention was provided by the tissue culture workshop of the Garden Flower Engineering Center of Chongqing University of Arts and Sciences.

[0019] 1. Screening of basic medium

[0020] Inoculate the subcultured seedlings of Lonicera chinensis Yulei No. 1 into the rooting medium for rooting culture; the basic medium of the rooting medium is 1 / 2MS, 1 / 2MB, 1 / 3MB, 1 / 4MB four kinds, add IAA and IBA with different concentrations and ratios, each medium was added with 30g / L sucrose, 6g / L agar and 50mg / L activated carbon, the pH was 5.8-6.0; 4 bottles were inoculated for each treatment, and 10 plants with the same growth status were inoculated in each bottle. For subcultured seedlings, the culture temperature is (20±1)°C, the lig...

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Abstract

The invention discloses a rooting culture medium and a rooting culture method for a tissue culture seedling of lonicera macranthoides Hand.Mazz Yulei #1. The rooting culture medium takes a 1 / 2MB culture medium as a basic culture medium and is added with 0.1mg / L of indoleacetic acid, 2.0mg / L of indol-3-butyric acid, 30g / L of sucrose, 6g / L of agar and 50mg / L of active carbon. Culture conditions of a rooting culture are as follows: the illumination time is 12 hours per day, the illumination intensity is 1500-2000Lx and the culture temperature is 20 DEG C. According to the rooting culture medium and the rooting culture method, the influences on the rooting of the tissue culture seedling of the lonicera macranthoides Hand.Mazz Yulei #1, caused by different basic culture mediums (MS and MB), hormones (IAA (Indoleacetic Acid) and IBA (Indol-3-Butyric Acid)) and culture temepraatures (20 DEG C and 25 DEG C), are researched to find a culture medium formula which is most suitable for the rooting of the tissue culture seedling of the lonicera macranthoides Hand.Mazz Yulei #1, so that the rooting rate is increased and technical supports are provided for industrial production.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a rooting culture medium and a rooting culture method for Lonicera chinensis Yulei No. 1 tissue culture seedlings. Background technique [0002] Lonicera macranthoides Hand.Mazz belongs to Lonicera L. in the family Caprifoliaceae, commonly known as Lonicera grandiflora. The content of chlorogenic acid in its flower buds is the highest among all kinds of honeysuckle in the country, and it is also the most popular species in Southwest and Central South regions. One of the main varieties of commercial honeysuckle is an important bulk Chinese herbal medicine in my country. It contains volatile oil, flavonoids, triterpene saponins, organic acids, etc., and has multiple functions such as anti-virus, anti-oxidation, and immunity enhancement. It is reported that the flower buds of more than 20 kinds of plants in the country are used as honeysuckle as medicine, all of which are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈泽雄
Owner CHONGQING UNIV OF ARTS & SCI
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