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Ethyl acetate extract of Lonicera macranthoides, preparation method and application thereof

A technology for extraction of ethyl acetate and Lonicera cinerea, applied in the field of ethyl acetate extract of Lonicera cinerea and its preparation, can solve the problems that have not yet been seen in the reports of ethyl acetate extract and its application, and achieve Excellent effect

Inactive Publication Date: 2014-07-09
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no report on the ethyl acetate extract of Lonicera cinerea and its application

Method used

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  • Ethyl acetate extract of Lonicera macranthoides, preparation method and application thereof
  • Ethyl acetate extract of Lonicera macranthoides, preparation method and application thereof
  • Ethyl acetate extract of Lonicera macranthoides, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Ethyl acetate extract of Lonicera cinerea

[0042] Grind honeysuckle, pass through a 35-mesh sieve (with a particle size of 500 μm), take 500 grams of coarse powder, soak in 1500 ml of petroleum ether for 24 hours, then percolate with petroleum ether, and extract by percolation until the color of the extract is light (ie Obtain 5 times the volume of the weight of Lonicera pilosula, about 2500ml of leachate), recover the residue, evaporate the solvent in the residue, and then use 80% ethanol for reflux extraction twice, each time using 1500ml of ethanol, each extraction time For 2 hours, combine the extracts, recover the ethanol under reduced pressure until there is no alcohol smell, and obtain the ethanol concentrate, then extract twice with ethyl acetate equal to the volume of the concentrate, collect the supernatant, and concentrate the combined supernatant to dryness, that is 1.1 g of ethyl acetate extract was obtained (0.22% yield).

Embodiment 2

[0043] Example 2: Ethyl acetate extract of Lonicera cinerea

[0044] Take honeysuckle and grind it, pass it through a 20-mesh sieve (particle size is 840 μm), soak in 1500ml of petroleum ether for 16h, and percolate with petroleum ether to obtain 3000ml of leachate and residue, evaporate the residue to dry petroleum ether, and then remove the residue Use 2000ml of 70% ethanol to extract twice, each time for 2 hours, combine the two extracts, recover the ethanol under reduced pressure until there is no alcohol smell, and obtain the ethanol concentrate, then extract with ethyl acetate equal to the volume of the concentrate for 2 Once, the supernatant was collected, and the combined supernatant was concentrated to dryness to obtain 1.16 g of ethyl acetate extract (yield: 0.232%).

Embodiment 3

[0045] Example 3: Ethyl acetate extract of Lonicera cinerea

[0046] Take honeysuckle and grind it, pass it through a 45-mesh sieve (particle size is 350 μm), soak it in 2500ml of petroleum ether for 36 hours, and percolate with petroleum ether to obtain 3500ml of leachate and residue, evaporate the residue to dry petroleum ether, and then remove the residue Use 2500ml of 85% ethanol to extract twice, each time for 2 hours, combine the two extracts, recover the ethanol under reduced pressure until there is no alcohol smell, and obtain the ethanol concentrate, then extract with ethyl acetate equal to the volume of the concentrate for 2 Once, the supernatant was collected, the combined supernatant was concentrated to dryness, and 1.275 g of ethyl acetate extract was obtained (the yield was 0.255%).

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PUM

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Abstract

The invention relates to an ethyl acetate extract of Lonicera macranthoides, a preparation method and application thereof. The extract is prepared by the method of: soaking Lonicera macranthoides by petroleum ether, then conducting percolation by petroleum ether, recovering the residue, volatilize the solvent in the residue, subjecting the residue to reflux extraction by ethanol, concentrating the extracted solution, then performing extraction with ethyl acetate, and collecting the supernatant, thus obtaining the ethyl acetate extract of Lonicera macranthoides. In vitro tests find that the ethyl acetate extract of Lonicera macranthoides provided by the invention can inhibit EGFR kinase activity, has obvious effect, and can resist skin cancer, leukemia, colon cancer, liver cancer, gastric cancer, breast cancer and other tumors.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to an ethyl acetate extract of Lonicera cinerea and its preparation method and application. Background technique [0002] Lonicera macranthoides is the dry flower bud or flower with the first bloom of Lonicera macranthoides Hand.-Mazz, a plant of the Lonicera family Lonicera. The 2010 edition of the Chinese Pharmacopoeia compares it with Lonicera hypoglauca Miq., Lonicera Confusa DC., and Lonicera Confusa DC. The dried flower buds or the first flowers of Lonicera fulvotomentosa Hsu et S.C.Cheng are included under the heading of Mountain Silver Flower. [0003] Lonicerae ash has the effect of clearing heat, detoxifying and evacuating wind-heat. It is mainly distributed in Guizhou, Sichuan, Hunan, Guangxi, Fujian and other places in my country. The main chemical components are phenolic acids, flavonoids, saponins and so on. Phenolic acids mainly include caffeic acid, chlorog...

Claims

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Application Information

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IPC IPC(8): A61K36/355A61P35/00G01N30/02G01N30/36
Inventor 陈士林黄林芳林森森任伟光卞兆祥徐宏喜杨大坚陈新滋
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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