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Method for extracting compound of phenolic acid from leaves of woodbind

A technology for honeysuckle leaves and plant leaves, applied in the field of chlorogenic acid and isochlorogenic acid, which can solve problems affecting the extraction and separation of chlorogenic acid and isochlorogenic acid, and achieve low cost, no harmful solvents, and high production safety Effect

Inactive Publication Date: 2007-02-14
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The resources of honeysuckle plant leaves are more abundant and cheap. Although the leaves of honeysuckle plants also contain chlorogenic acid and isochlorogenic acid, they contain a lot of impurities such as oil, tannins, polysaccharides, proteins, and chlorophyll. Extraction and separation of chlorogenic acid and isochlorogenic acid

Method used

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  • Method for extracting compound of phenolic acid from leaves of woodbind
  • Method for extracting compound of phenolic acid from leaves of woodbind
  • Method for extracting compound of phenolic acid from leaves of woodbind

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1 Assay method

[0032]Instruments and reagents High performance liquid chromatograph Aglient1100 series, binary pump G1312A, autosampler, diode array detector, Chemstation A.06.01 chemistry workstation, chromatographic column: Aglient Zorbax 80A, Extend-C18 (4.6mm×250mm, 5cm ), experimental water (Guangzhou Robust Food and Beverage Co., Ltd.), acetonitrile is chromatographically pure, and phosphoric acid is analytically pure.

[0033] Chromatographic conditions: mobile phase: 0.2% phosphoric acid solution-acetonitrile (88:12), maintained for 0-12min, linearly changed to (83:17) at 12-15min, and maintained the ratio (83:17) at 15-30min; 30-60min linearly changed to (50:50). The flow rate is 1.0mL·min -1 , The detection wavelength is 327nm, and the column temperature is 30°C.

[0034] 2 choice of medicinal materials

[0035] In the honeysuckle genus Lonicera japonicus, L. hypoglauca, L. confusa, L.similes, L. dasystyla, and L. honeysuckle (L. L. chrysantha) can be used to ex...

Embodiment 2

[0054] Take 20kg of Lonicera japonicus leaf medicinal material, pulverize it into 40 mesh powder, extract with petroleum ether at 80℃ for 6 hours to degrease, and extract 8 times the volume of 60% ethanol by the weight of the medicinal material, heat reflux at 85℃ for two times, 1 time each time hour. The extracts were combined, and the solvent was recovered under reduced pressure. The obtained concentrated solution was segmented by D101 macroporous resin, first eluted with water, and then eluted with 10% and 35% ethanol in sequence. Combine water and 10% alcohol eluent, after concentration, first add ethanol to 50% alcohol content, alcohol precipitation, remove the precipitate, the filtrate is concentrated to (70 ℃) relative density of 1.08, then add ethanol to the alcohol content It is 80%, alcohol precipitation, centrifugation to take the supernatant, concentration and vacuum drying to obtain 1.30kg of crude chlorogenic acid with a purity of 70.3%.

[0055] In addition, the 35%...

Embodiment 3

[0058] Take 30 kg of Lonicera japonicus leaf medicinal material, pulverize it into 40 mesh powder, extract with petroleum ether at 85°C for 5 hours by Soxhlet method for degreasing, and extract the medicinal material with 10 times the volume of 75% ethanol under hot reflux for two times, each time for 1 hour. The extract was decompressed to recover the solvent, the concentrated solution was separated by D101 macroporous resin, and directly eluted with 10% ethanol to collect the 10% ethanol eluate; then eluted with 40% ethanol to collect the 40% ethanol eluate. Take 10% ethanol eluate, after concentration, first add ethanol to 50% alcohol content, alcohol precipitation, remove the precipitate, the filtrate is concentrated to (70 ℃) relative density of about 1.10, then add ethanol to the alcohol content of 80 %, alcohol precipitation, centrifugation to take the supernatant, concentration and vacuum drying to obtain 1.9 kg of crude chlorogenic acid with a purity of 72.5%.

[0059] In...

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Abstract

The preparation method of chlorogenic acid and isochlorogenic acid compound uses leaf of lonicera macranthoides as raw material and adopts the following steps: defatting, using ethyl alcohol with a certain concentration to make extraction, concentrating ethyl alcohol extract, feeding the concentrated ethyl alcohol extract into macroporous resin column, eluting by using 0-10% ethyl alcohol to obtain chlorogenic acid and its isomer, then eluting with 20-35% ethyl alcohol to obtain isochlorogenic acid and its isomer; mixing then according to different ratio and making treatment so as to obtain total phenelic acid, its content can be up to 80%.

Description

Technical field [0001] The invention belongs to a method for extracting phenolic acid compounds from the leaves of the honeysuckle plant, and specifically relates to a method for extracting and separating chlorogenic acid and isochlorogenic acid with strong anti-radical and anti-oxidant effects from the leaves of the honeysuckle plant. Background technique [0002] The chlorogenic acid and isochlorogenic acid (DCQ or dicaffeoylquinic acid) and their derivatives in honeysuckle have antiviral effects (King PJ, Ma G, Miao W, et al. Structure-activity relationships: analogues of thedicaffeoylquinic and dicaffeoyltartaric acids as potent inhibitors of humanimmunodeficiency virus type 1 intergerase and repiciation[J]J.Med Chem, 1999, 42(3):497-509), in addition to having a strong anti-free radical effect, Heilmann and Weiss etc. Human neutrophils are stimulated by zymosan or FMLP to produce respiratory bursts, and the generation of oxygen free radicals is detected. It is found that DCQ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/58
Inventor 李萍黄雄毕志明李会军
Owner CHINA PHARM UNIV
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