A root cutting seedling raising method for Lonicera japonica

Abstract

The present application provides a method for raising seedlings of honeysuckle by root cuttings. The method comprises the following steps: (1) selecting healthy, pest-free, and 0.3 cm thick root strips from a mother tree as cuttings, cutting the root strips into 5-8 cm long strips and disinfecting them; (2) after disinfection, quickly dipping the base of the cuttings with a plant rooting agent, and then soaking the cuttings in a composite nutrient solution comprising the following raw materials: loofah extract, Ailanthone, Acanthopanax senticosus polysaccharide, and ethanol; taking the cuttings out after soaking for 10-15 minutes before cutting; (3) spraying the cuttings alternately with a composite agent and a fungicide every 5-7 days after cutting, at night when the leaf surface is free of water, wherein the composite agent is prepared by extracting gentian, akebia, and kudzu root. The method of the present invention effectively increases the growth rate of honeysuckle seedlings, improves disease resistance to diseases such as brown spot disease, white rot, powdery mildew, etc., promotes healthy and rapid growth, and improves seedling survival rate and seedling quality.

Description

Technical Field

[0001] The present application relates to the field of plant biology, and in particular to a root cutting seedling cultivation method for Lonicera japonica. Background Art

[0002] Gray felt honeysuckle (Lonicera macranthoides) is the dried buds or initially opened flowers of the gray felt honeysuckle plant of the genus Lonicera of the family Caprifoliaceae.

[0003] Honeysuckle (Lonicera japonica) is a member of the genus Lonicera in the family Caprifoliaceae. It is one of the botanical sources of the medicinal material "Lonicera japonica" in the Chinese Pharmacopoeia and is used clinically as a source of the traditional Chinese medicine "Lonicera japonica." It is extensively cultivated in southwestern and central-southern my country and is a mainstream commercial product of the medicinal honeysuckle. It has the effects of clearing heat and detoxifying, cooling and dispersing wind-heat, and is primarily used for carbuncles, sore throat, erysipelas, and dysentery caused by heat and blood. Currently, the production of seedlings of Honeysuckle (Lonicera japonica) relies primarily on cutting propagation. While extensive research has been conducted on cutting propagation techniques, common diseases associated with this method include root rot, gray mold, root-knot nematode disease, anthracnose, and gray leaf spot, which can easily lead to leaf wilting and, in severe cases, seedling death. Summary of the Invention

[0004] In view of this, the present application proposes a root cutting seedling raising method of Lonicera japonica to solve the above problems.

[0005] The technical solution of this application is achieved as follows:

[0006] A root cutting seedling raising method for Lonicera japonica comprises the following steps:

[0007] (1) Select healthy, pest-free, and 0.3 cm thick root cuttings from mother trees as cuttings. Cut the cuttings into 5-8 cm long and sterilize them.

[0008] (2) After disinfection, the base of the cuttings is quickly dipped in a plant rooting agent, and then the cuttings are soaked in a composite nutrient solution, wherein the composite nutrient solution comprises the following raw materials in parts by weight: 10-20 parts of loofah extract, 20-30 parts of Ailanthone, 5-10 parts of Acanthopanax senticosus polysaccharide, and 90-110 parts of ethanol; after soaking, the cuttings are taken out and then the cuttings are grafted after 10-15 minutes;

[0009] (3) Every 5 to 7 days after cutting, when there is no water on the leaf surface at night, spray the compound agent and fungicide alternately. The compound agent is prepared by extracting gentian, akebia, and kudzu root in a mass ratio of 1.8-2.2:3.5-4.5:2.8-3.2.

[0010] A further technical solution is that in step (2), the plant rooting agent is sodium nitrate naphthaleneacetic acid plant rooting agent, which is diluted 2000-3000 times before use.

[0011] A further technical solution is that, in step (2), the time of the rapid dipping is 10 to 15 seconds.

[0012] A further technical solution is that, in step (2), the preparation method of the composite nutrient solution is as follows: adding loofah extract, Acanthopanax senticosus polysaccharide and Ailanthus altissima extract to ethanol, mixing them evenly, and preparing the target composite nutrient solution.

[0013] A further technical solution is that in step (2), the compound nutrient solution is diluted 1000-2000 times before use.

[0014] A further technical solution is that in step (2), the soaking time is 10 to 15 seconds.

[0015] A further technical solution is that in step (2), the cutting matrix is prepared from peat soil, vermiculite and Croton rotundus in a mass ratio of 1.9-2.1:0.9-1.1:0.9-1.1, and the matrix is filled in a seedling tray.

[0016] A further technical solution is that, in step (3), the preparation method of the composite medicament is as follows: taking Chinese medicinal materials of gentian, akebia, and kudzu root, crushing them, and subjecting them to microwave extraction, with a microwave extraction power of 450-550W, a microwave extraction time of 10-15min, a microwave extraction solvent of 70%-80% v / v ethanol solution, a material-liquid ratio g / ml of 1:8-12, and concentrating under reduced pressure after extraction to obtain a composite Chinese medicine extract to prepare the composite medicament.

[0017] A further technical solution is that, in step (3), the compound agent is diluted with water to 2500-3000 times before use, and the dosage is 40-60 g per mu.

[0018] A further technical solution is that in step (3), the fungicide is 72% cymoxanil zinc or 70% thiophanate methyl, which is diluted 1200-1500 times and sprayed at a dosage of 80-120 g per mu.

[0019] Compared with the prior art, the present invention has the following advantages:

[0020] (1) The root cutting seedling raising method of the present invention is adopted, a composite nutrient solution prepared by using loofah extract, Ailanthone, Acanthopanax senticosus polysaccharide, etc., a composite agent prepared by using gentian, Akebia, and Pueraria root, and a fungicide are used to effectively increase the growth rate of honeysuckle seedlings, improve the disease resistance to diseases such as brown spot disease, white rot, powdery mildew, promote healthy and rapid growth, and improve the survival rate and quality of seedlings.

[0021] (2) By adopting the seedling raising method of the present invention, the survival rate of the seedlings of the gray felt honeysuckle is high, the incidence rates of brown spot disease, white rot and powdery mildew are low, and the transplanting survival rate of the gray felt honeysuckle is high, the seedling quality is high, and the seedling raising effect is good.

[0022] (3) The seedling raising method of the present invention can reduce the use of traditional fungicides, increase the number of times the cutting matrix is reused, and improve resource utilization while ensuring the quality of seedling raising; and reduce the use of chemical agents and reduce the risk of environmental pollution. DETAILED DESCRIPTION

[0023] In order to better understand the technical content of this application, specific embodiments are provided below to further illustrate this application.

[0024] Unless otherwise specified, the experimental methods used in the examples of this application are all conventional methods.

[0025] Unless otherwise specified, the materials, reagents, etc. used in the examples of this application can be obtained from commercial channels.

[0026] The 72% cymoxanil zinc or 70% thiophanate methyl used in the examples of the present application are both commercially available products.

[0027] The sodium nitrate and naphthaleneacetic acid plant rooting agent used in the examples of the present application is a commercially available 2.85% sodium nitrate and naphthaleneacetic acid aqueous solution.

[0028] The Cistanche deserticola used in the examples of the present application is prepared by drying and crushing the whole Cistanche deserticola herb.

[0029] Preparation Example 1

[0030] The composite nutrient solution comprises the following raw materials in parts by weight: 15 parts of luffa extract, 25 parts of ailanthin, 8 parts of acanthopanax polysaccharide and 100 parts of anhydrous ethanol; the preparation method of the composite nutrient solution comprises the following steps: adding the luffa extract, acanthopanax polysaccharide and acanthus polysaccharide to anhydrous ethanol in sequence, mixing them evenly, and preparing the target composite nutrient solution.

[0031] Preparation Example 2

[0032] The composite nutrient solution comprises the following raw materials in parts by weight: 13 parts of luffa extract, 30 parts of ailanthin, 5 parts of acanthopanax polysaccharide and 100 parts of anhydrous ethanol; the preparation method of the composite nutrient solution comprises the following steps: adding the luffa extract, acanthopanax polysaccharide and acanthus polysaccharide to anhydrous ethanol in sequence, mixing them evenly, and preparing the target composite nutrient solution.

[0033] Preparation Example 3

[0034] The composite nutrient solution comprises the following raw materials in parts by weight: 18 parts of luffa extract, 20 parts of ailanthin, 10 parts of acanthopanax polysaccharide and 100 parts of anhydrous ethanol; the preparation method of the composite nutrient solution comprises the following steps: adding the luffa extract, acanthopanax polysaccharide and acanthus polysaccharide to anhydrous ethanol in sequence, mixing them evenly, and preparing the target composite nutrient solution.

[0035] Preparation Example 4

[0036] The composite medicine is prepared by extracting gentian, akebia and kudzu root in a mass ratio of 2.0:4.0:3.0; the preparation method of the composite medicine comprises the following steps: taking gentian, akebia and kudzu root Chinese medicinal materials, crushing them, and subjecting them to microwave extraction, wherein the microwave extraction power is 500W, the microwave extraction time is 12 minutes, the microwave extraction solvent is 75% v / v ethanol solution, the material-liquid ratio g / ml is 1:10, and after extraction, the composite Chinese medicine extract is obtained by concentrating under reduced pressure to prepare the composite medicine.

[0037] Preparation Example 5

[0038] The composite medicine is prepared by extracting gentian, akebia and kudzu root in a mass ratio of 1.8:4.0:3.2; the preparation method of the composite medicine comprises the following steps: taking gentian, akebia and kudzu root Chinese medicinal materials, crushing them, and subjecting them to microwave extraction, wherein the microwave extraction power is 450W, the microwave extraction time is 15 minutes, the microwave extraction solvent is 75% v / v ethanol solution, the material-liquid ratio g / ml is 1:8, and after extraction, the composite Chinese medicinal extract is concentrated under reduced pressure to obtain the composite Chinese medicinal extract, thereby preparing the composite medicine.

[0039] Preparation Example 6

[0040] The composite medicine is prepared by extracting gentian, akebia and kudzu root in a mass ratio of 2.2:4.0:2.8; the preparation method of the composite medicine comprises the following steps: taking gentian, akebia and kudzu root Chinese medicinal materials, crushing them, and subjecting them to microwave extraction, wherein the microwave extraction power is 550W, the microwave extraction time is 10 minutes, the microwave extraction solvent is 75% v / v ethanol solution, the material-liquid ratio g / ml is 1:12, and after extraction, the composite Chinese medicinal extract is obtained by concentrating under reduced pressure to prepare the composite medicine.

[0041] Example 1

[0042] A root cutting seedling raising method for Lonicera japonica comprises the following steps:

[0043] (1) Select healthy, pest-free, and 0.3 cm thick root strips from the mother tree as cuttings. Cut the root strips into 5-8 cm long and disinfect them.

[0044] (2) After disinfection, use a plant rooting agent (2.85% sodium nitrate·naphthaleneacetic acid aqueous solution, diluted 2500 times before use) to quickly dip the base of the cuttings for 13 seconds, then soak the cutting leaves in the compound nutrient solution of Preparation Example 1 (diluted 1500 times before use) for 13 seconds, take them out after soaking and then carry out cuttings 12 minutes later. The cutting medium is made of peat soil, vermiculite and Glechoma longituba in a mass ratio of 2:1:1, and the medium is filled in the seedling hole tray.

[0045] (3) Every 6 days after cutting, at night when there is no water on the leaf surface, spray the compound agent of Preparation Example 4 (original dosage 50g / mu, diluted 2500 times with water) and 72% cymoxanil manganese zinc (original dosage 100g / mu, diluted 1200 times) alternately once, that is, spray the compound agent after cutting, spray 72% cymoxanil manganese zinc after 6 days, and spray the compound agent after another 6 days, and so on.

[0046] Example 2

[0047] A root cutting seedling raising method for Lonicera japonica comprises the following steps:

[0048] (1) Select healthy, pest-free, and 0.3 cm thick root strips from the mother tree as cuttings. Cut the root strips into 5-8 cm long and disinfect them.

[0049] (2) After disinfection, use a plant rooting agent (2.85% sodium nitrate·naphthaleneacetic acid aqueous solution, diluted 2000 times before use) to quickly dip the base of the cuttings for 10 seconds, then soak the cutting leaves in the compound nutrient solution of Preparation Example 2 (diluted 1500 times before use) for 15 seconds, take them out after soaking and then carry out cuttings 10 minutes later. The cutting medium is made of peat soil, vermiculite and Glechoma longituba in a mass ratio of 2:1:1, and the medium is filled in the seedling hole tray.

[0050] (3) Every 6 days after cutting, at night when there is no water on the leaf surface, spray the compound agent of Preparation Example 5 (original dosage 50g / mu, diluted 2500 times with water) and 70% methyl thiophanate (original dosage 100g / mu, diluted 1200 times) alternately once, that is, spray the compound agent after cutting, spray 70% methyl thiophanate after 6 days, and spray the compound agent after another 6 days, and so on.

[0051] Example 3

[0052] A root cutting seedling raising method for Lonicera japonica comprises the following steps:

[0053] (1) Select healthy, pest-free, and 0.3 cm thick root strips from the mother tree as cuttings. Cut the root strips into 5-8 cm long and disinfect them.

[0054] (2) After disinfection, use a plant rooting agent (2.85% sodium nitrate·naphthaleneacetic acid aqueous solution, diluted 3000 times before use) to quickly dip the base of the cuttings for 15 seconds, then soak the cutting leaves in the compound nutrient solution of Preparation Example 3 (diluted 1500 times before use) for 10 seconds, take them out after soaking and then carry out cuttings 15 minutes later. The cutting medium is made of peat soil, vermiculite and Glechoma longituba in a mass ratio of 2:1:1, and the medium is filled in the seedling hole tray.

[0055] (3) Every 6 days after cutting, at night when there is no water on the leaf surface, spray the compound agent of Preparation Example 6 (original dosage 50g / mu, diluted 2500 times with water) and 72% cymoxanil manganese zinc (original dosage 100g / mu, diluted 1200 times) alternately once, that is, spray the compound agent after cutting, spray 72% cymoxanil manganese zinc after 6 days, and spray the compound agent after another 6 days, and so on.

[0056] Comparative Example 1

[0057] The difference from Example 1 is that: the composite agent. The raw materials for preparing the composite agent do not contain Pueraria lobata. The composite agent is prepared by extracting Gentiana scabra and Akebia kebia in a mass ratio of 5:4. The composite agent is prepared by extracting Gentiana scabra and Akebia kebia in a mass ratio of 5:4. The preparation method of the composite agent is as follows: Gentiana scabra and Akebia kebia are taken, crushed, and subjected to microwave extraction at a microwave extraction power of 500W and a microwave extraction time of 12 minutes. The microwave extraction solvent is 75% v / v ethanol solution, and the material-liquid ratio is 1:10. After extraction, the composite agent is concentrated under reduced pressure to obtain a composite Chinese herbal extract, thereby obtaining the composite agent. Other operations are consistent with those in Example 1.

[0058] Comparative Example 2

[0059] The difference from Example 1 is that the raw materials for preparing the composite agent do not contain Akebia. The composite agent is prepared by extracting Gentiana scabra and Pueraria lobata in a mass ratio of 5:4. The composite agent is prepared by extracting Gentiana scabra and Pueraria lobata in a mass ratio of 5:4. The preparation method of the composite agent is as follows: Gentiana scabra and Pueraria lobata are taken, crushed, and subjected to microwave extraction at a microwave power of 500W and a microwave extraction time of 12 minutes. The microwave extraction solvent is 75% v / v ethanol solution, and the material-liquid ratio is 1:10. After extraction, the extract is concentrated under reduced pressure to obtain a composite Chinese herbal extract, thereby preparing the composite agent. Other operations are consistent with those in Example 1.

[0060] Comparative Example 3

[0061] The difference from Example 1 is that the composite nutrient solution does not contain Ailanthone. The composite nutrient solution is prepared by including the following raw materials in parts by weight: 30 parts of luffa extract, 18 parts of Acanthopanax senticosus polysaccharide, and 100 parts of anhydrous ethanol. The composite nutrient solution is prepared by adding the luffa extract and Acanthopanax senticosus polysaccharide to anhydrous ethanol and mixing them uniformly to prepare the composite nutrient solution. All other operations are the same as in Example 1.

[0062] Comparative Example 4

[0063] The difference from Example 1 is that the composite nutrient solution does not contain Acanthopanax senticosus polysaccharide. The composite nutrient solution is prepared by including the following raw materials in parts by weight: 23 parts of luffa extract, 25 parts of Ailanthone, and 100 parts of anhydrous ethanol. The composite nutrient solution is prepared by adding the luffa extract and Ailanthone to anhydrous ethanol and mixing them uniformly to prepare the composite nutrient solution. All other operations are the same as in Example 1.

[0064] Comparative Example 5

[0065] The difference from Example 1 lies in the ratio of the raw materials of the composite agent. The composite agent is prepared by extracting gentian, akebia, and kudzu root in a mass ratio of 3:3:3. Preparation method of the composite agent: Gentian, akebia, and kudzu root are ground and microwave-extracted at a microwave power of 500W for 12 minutes, a material-liquid ratio of 1:10 (g / ml), and a 75% v / v ethanol solution as the microwave extraction solvent. After extraction, the composite Chinese medicine extract is concentrated under reduced pressure to obtain the composite Chinese medicine extract, thereby preparing the composite agent. Other operations are consistent with those of Example 1.

[0066] Comparative Example 6

[0067] The difference from Example 1 is the raw material ratio of the composite nutrient solution. The composite nutrient solution is prepared by including the following raw materials in parts by weight: 20 parts of luffa extract, 20 parts of Ailanthone, 20 parts of Acanthopanax senticosus polysaccharide, and 100 parts of anhydrous ethanol. Preparation method of the composite nutrient solution: Add the luffa extract, Acanthopanax senticosus polysaccharide, and Ailanthone to anhydrous ethanol and mix well to prepare the composite nutrient solution. Other operations are consistent with those in Example 1.

[0068] Test example

[0069] Test site: Longhui County, Hunan Province

[0070] Trial start time: March 2023

[0071] First, experiments were conducted using the root cutting seedling cultivation methods of Lonicera japonica (Grey Honeysuckle) according to Examples 1-3 and Comparative Examples 1-6. Examples 1-3 and Comparative Examples 1-6 were designated as Test Groups 1-9, respectively. Each test group had 1,000 seedlings, and seedlings emerged 30 days after cutting. Thirty days after cutting, the survival and disease incidence of Lonicera japonica seedlings were analyzed, along with the seedling survival rate and the incidence of brown spot disease, white rot, and powdery mildew.

[0072] Seedling survival rate = (number of surviving seedlings / number of seedlings) * 100

[0073] Incidence rate = (number of diseases / number of seedlings) * 100

[0074] Table 1 The number of survival and diseased honeysuckle seedlings

[0075]

[0076]

[0077] Table 2 Survival rate and disease incidence of Lonicera japonica seedlings

[0078]

[0079] As shown in Table 1-2, the survival rate of the seedlings of Examples 1-3 was high, and the number of plants infected with brown spot disease, white rot, and powdery mildew was relatively small, indicating good seedling cultivation results. Among them, the incidence of brown spot disease, white rot, and powdery mildew in Comparative Examples 1-6 was relatively high, especially in Comparative Example 1-4.

[0080] 2. Among the surviving seedlings, 100 plants were randomly selected for transplanting in each experimental group, with 3 replicates, and the survival rate was calculated 2 weeks after transplanting.

[0081] Transplant survival rate = (number of live seedlings after transplanting / number of transplants) * 100

[0082] Table 3 Transplant survival of honeysuckle seedlings

[0083]

[0084] The results are shown in Table 3. The transplant survival rates of the honeysuckle of Examples 1-3 were higher and the seedling quality was higher. The transplant survival rates of the honeysuckle of Comparative Examples 1-4 were significantly lower.

[0085] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A root cutting seedling raising method for Lonicera japonica, characterized in that: The following steps are involved: (1) Select healthy, pest-free, and 0.3 cm thick root cuttings from mother trees as cuttings. Cut the cuttings into 5-8 cm long and sterilize them. (2) After disinfection, use a plant rooting agent to quickly dip the base of the cuttings, and the time for the quick dipping is 10 to 15 seconds; then soak the cutting leaves in a composite nutrient solution for 10 to 15 seconds, the composite nutrient solution comprising the following raw materials in parts by weight: 15 parts of loofah extract, 25 parts of Ailanthus altissima, 8 parts of Acanthopanax senticosus polysaccharide and 100 parts of anhydrous ethanol; after soaking, take out the cuttings and then carry out cuttings 10 to 15 minutes later, the cutting matrix is made of peat soil, vermiculite and Acanthopanax senticosus in a mass ratio of 2:1:1, and the matrix is filled in a seedling hole tray; the plant rooting agent is sodium nitrate naphthaleneacetic acid plant rooting agent, which is diluted 2000-3000 times before use; the preparation method of the composite nutrient solution is: add loofah extract, Acanthopanax senticosus polysaccharide and Ailanthus altissima to ethanol, mix evenly, and prepare the target composite nutrient solution; the composite nutrient solution is diluted 1000-2000 times before use; (3) Every 5 to 7 days after cutting, at night when there is no water on the leaf surface, spray the compound agent and the fungicide alternately. The compound agent is prepared by extracting gentian, akebia and kudzu root in a mass ratio of 2:4:3; the preparation method of the compound agent is as follows: take gentian, akebia and kudzu root Chinese medicinal materials, crush them, and extract them by microwave, the microwave extraction power is 500W, the microwave extraction time is 12min, the microwave extraction solvent is 75% v / v ethanol solution, the material-liquid ratio g / ml is 1:10, and after extraction, reduce pressure and concentrate to obtain a compound Chinese medicinal extract to prepare the compound agent; the compound agent is diluted with water to 2500-3000 times before use, and the dosage per mu is 40-60g; the fungicide is 72% mancozeb cymoxanil or 70% thiophanate-methyl, diluted 1200-1500 times and sprayed, and the dosage per mu is 80-120g.

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