30 results about "Tuberculosis bacillus" patented technology

The invention provides a tubercle bacillus drug tolerance detection reagent kit and method. The tubercle bacillus drug tolerance detection reagent kit comprises a tubercle bacillus drug tolerance detection reagent, wherein the tubercle bacillus drug tolerance detection reagent comprises a sequencing primer in accordance with a tubercle bacillus drug tolerance gene; the tubercle bacillus drug tolerance gene comprises one or more genes of rpoB, katG, inhA-promoter, inhA-structural, furA, embB, ubiA, pncA, rpsA, gyrA, gyrB, eis, rpsL, rrs, tlyA, rplC and rrl; and further, the reagent kit also contains a tubercle bacillus nucleic acid detection reagent, and the tubercle bacillus nucleic acid detection reagent comprises a primer pair 1 in accordance with IS6110, a primer pair 2 in accordance with the IS6110 and a probe primer in accordance with the IS6110. Through the adoption of the tubercle bacillus drug tolerance detection reagent kit disclosed by the invention, tubercle bacillus nucleicacid in samples can be quickly detected, and positive samples can be further subjected to drug tolerance detection; and the tubercle bacillus drug tolerance detection reagent kit has good sensitivity, good specificity and good accuracy, and can perform mutation detection on 48 sites of 17 drug tolerance genes of common antituberculosis drugs and fragment deficiency detection of an intergenic region, so that the tuberculosis medication can be more accurately and comprehensively guided.

The present invention provides the novel anti-IRC85 monoclonal antibody specifically binding with IRC85 and it showed potent effect in removing the infected / phagocytosed bacteria from THP-I, a monocytic cell that expresses human IRC85 and is infected with Listeria monocytogenes or WR-tubercle bacillus. Accordingly, it can be useful as a medicament and health care food in the prevention and treatment of tuberculosis disease and enteritis disease.

A Chinese medicine in the form of pill for treating pulmonary tuberculosis is prepared from verdigris, alum, gypsum, zedoary, wheat bran and / or flour, and edible vinegar through grinding the first four, mixing, adding heated vinegar, adding wheat bran and / or flour, stirring , pilling and baking.

The invention provides an anti-tuberculous compound with a structure as shown in a formula I. The compound is an s-triazolo-thiadiazole derivative (IMB-SDb8) when n in the formula I is equal to 0, andthe compound is an s-triazolo-thiadiazine derivative (IMB-SDc9) when n in the formula I is equal to 1. According to activity evaluation results in an embodiment, the compound IMB-SDb8 and IMB-SDc9 has definite anti-tuberculous bacillus activity and a wide application prospect in the aspect of preparation of anti-tuberculous medicines and is good in bioavailability and low in toxicity.

The invention discloses a Bacillus tuberculosis fusion protein and the producing method thereof and its use in the preparation of vaccine. The amino acid sequence of the said protein is shown as in SEQ ID No.:2. The construction of the said fusion protein is directed to the immune response of Bacillus tuberculosis, so as to increase the vaccine's protection efficacy. The said fusion protein can be mixed with adjuvant so as to increase the anti-tuberculosis protection efficacy via inducing the body fluid and cell immune response of Th1 type.

The invention discloses a preparation method and application of a MC-ARMS-mMB triplet technology in a drug-resistant tuberculosis diagnosis kit, and describes the MC-ARMS-mMB (microcolony-amplification-impeding mutation system-multiplex molecular beacon) triplet technology , a diagnostic kit for rapid culture of Mycobacterium tuberculosis and its drug-resistant bacteria. At the same time, the preparation method of the diagnostic kit was clarified; and a scientific presentation was made on its clinical application. It is particularly important to point out the clinical application value and better development prospect of the diagnostic kit.

The invention belongs to the technical field of biological agents and discloses a novel biological agent for treating drug resistant tuberculosis and a preparation method thereof. The novel biologicalagent for treating drug resistant tuberculosis is a tuberculosis specific lymphocyte and is prepared by utilizing IL-1 alpha, IL-2, a CD3 monoclonal antibody and tuberculosis specific antigens (CFP10, ESAT6). Compared with the conventional tuberculosis treatment method, the novel biological agent disclosed by the invention is reported for the first time at home and abroad, and obvious curative effects are achieved when patients with pulmonary tuberculosis are treated by utilizing lymphocytes cultured in vitro. For patients subjected to cell therapy, the number of lymphocytes in peripheral blood is obviously increased, the sputum examination result is rapidly turned to be negative, pulmonary shadow is rapidly absorbed, the course of disease is shortened, the mental state is improved, and the appetite and weight are increased. The living quality of the patients in anti-tuberculosis therapy is obviously improved.

The invention relates to a bone rehabilitating material and a preparation method of the material. The invention provides a bone rehabilitating material, the effective components of which are prepared by the following weights and portions of raw materials: 40 of a PLGA; 20 to 80 of a semi-hydrated calcium sulphate; 2 to 8 of a rifampicin. The invention also provides the preparation method of the bone rehabilitating material. The invention mixes and solidifies the semi-hydrated calcium sulphate, the PLGA, the rifampicin and a sodium chloride granule and digests the sodium chloride granule to make a novel multi-hole bone rehabilitating material with a function of anti-bacillus tuberculosis.

The invention relates to the technical field of traditional Chinese medicines, in particular to a medicine for treating pulmonary tuberculosis, which is prepared from Japanese raspberry root, radices stellariae dichotomae, sweet wormwood herb, bletilla striata and large-leaved gentian. The preparation method comprises three steps of decoction preparation, medicine powder preparation and compounding. Compared with the prior art, the medicine for treating pulmonary tuberculosis has the following beneficial effects that the medicine for treating pulmonary tuberculosis takes pure natural traditional Chinese medicines as raw materials, all the raw materials are in mutual compatibility in a monarch, minister, assistant and guide manner, and decoction and medicine powder are mutually matched, so that the effect of treating pulmonary tuberculosis can be achieved, and meanwhile, the medicine is rapid in effect, exact in curative effect and free of obvious side effects; liver and kidney function damage and drug resistance caused by western medicines are also avoided; meanwhile, the Japanese raspberry root is taken as a main component, medicine properties are extracted from the bottom of the Japanese raspberry root, and after the medicine is taken for one treatment course, the tuberculosis bacillus can be obviously cured, and the symptoms such as low fever, cough, hematemesis and abnormal sweating caused by existing symptoms are changed.

The present invention relates to a series of diphenyl oxide derivatives and a use of the diphenyl oxide derivatives in preparation of anti-tuberculosis drugs, and belongs to the field of chemical medicine. Specifically the present invention relates to a use of a compound represented by a formula (I), wherein Ar1 is a substituted benzene ring, n is an integer of 0-3, R1 is a substituted benzene ring Ar2 or a substituted heterocyclic Het. The test results show that the compound represented by the formula (I) provides good in vitro inhibition activity for tubercle bacillus H37Rv, provides a good treatment effect for in vivo BCG infection mice model, and provides a new selection for preparation of the anti-tuberculosis drugs.

The invention relates to a processing method of croton for treating tuberculosis, in particular to a high-temperature processing and attenuation method for traditional Chinese medicine croton and action thereof on treatment of the tuberculosis. The croton is processed through three methods of high-temperature frying, high-temperature roasting and microwave heating to obtain a croton processed product. In the processed croton, toxic protein is not detected, LD50 is obviously increased, the relative content of each component in croton oil is basically kept unchanged, and the croton oil content is relatively increased. After being combined with other traditional Chinese medicines to prepare pills, the processed croton has an obvious anti-tuberculosis bacillus effect in vivo and in vitro. Theprocessing method of the croton for treating the tuberculosis disclosed by the invention has the effects of reducing toxicity and enhancing therapeutic effect; and compared with the conventional croton processing methods of roasting, boiling, steaming and the like, the processing method has the advantages of prominent advantages.

The invention discloses avain tuberculosis hyper-immune serum and a preparation method thereof. The preparation method includes the steps that inactivated avain tuberculosis bacilli are directly adopted as immunizing antigens, immunizing is conducted with an ear edge intravenous injection method, immunizing is conducted once every three days, immunizing is conducted seven times in total, blood sampling is conducted seven days after the last time of immunizing, and serum is collected. The high-potency serum can be prepared only through the inactivated bacilli via intravenous injection on immune animals, emulsifying operation is not needed, the whole hyper-immune serum preparation process only lasts for 26 days, the antibody valence measured by a tube agglutination test reaches 1 / 128, and compared with a conventional hyper-immune serum preparation method, the requirements for preparation conditions for the immunizing antigens involved in the method are low, immunizing procedure operation is simpler, needed time is shorter, and the high-potency antibody level can be acquired.

The invention discloses a use of chimonanthus plant anti-mycobacterium tuberculosis, and particularly relates to application of a chimonanthus plant and an extractive thereof for preparing pharmaceutical for anti-mycobacterium tuberculosis and diseases caused by the anti-mycobacterium tuberculosis. The chimonanthus plant comprises willow-pattern calyx canthus, Zhejiang calyx canthus and mountain calyx canthus. The extractive comprises a water extractive, an alcohol extractive, a steam distillation extractive and a supercritical extractive.

The present invention relates to the technical field of biopharmaceuticals, in particular to the application of TREM-2+T cells in the preparation of drugs for treating tuberculosis. The drug can realize immunotherapy for tuberculosis, reduce pulmonary tuberculosis, and reduce inflammation; in addition, because TREM-2+ T cells are derived from the patient's autologous peripheral blood, there is no rejection, and the reaction is suitable for comprehensive treatment of tuberculosis; TREM-2+ T cells for the treatment of tuberculosis are prepared through the process of cell sorting, interleukin-2 stimulation in vitro culture expansion and washing, and the preparation process is more scientific and efficient.