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Use of Bacteroides fragilis in the preparation of drugs for the treatment and prevention of tuberculosis

A technology for Bacteroides fragilis, tuberculosis, applied in the field of biomedicine

Inactive Publication Date: 2019-01-15
REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, there are no reports and patent applications of Bacteroides fragilis for the auxiliary prevention and treatment of tuberculosis

Method used

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  • Use of Bacteroides fragilis in the preparation of drugs for the treatment and prevention of tuberculosis
  • Use of Bacteroides fragilis in the preparation of drugs for the treatment and prevention of tuberculosis
  • Use of Bacteroides fragilis in the preparation of drugs for the treatment and prevention of tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Culture of Bacteroides fragilis

[0037] training method

[0038] Step 1: Take a freeze-dried strain of Bacteroides fragilis (Bacteroides fragilis), add 200 μL of TSB medium, redissolve, absorb 20 bases, draw a line on the blood plate, and put it in the biochemical incubator after the gas control system of the anaerobic tank is pumped 37 dishes, anaerobic culture for 48 hours;

[0039] Step 2: Pick a monoclonal colony and insert it into 10mL TSB medium, 37, and anaerobic culture for 12h;

[0040] Step 3: Take 1 bottle of 500mL TSB medium, insert 1% (v / v) bacteria, 37, and anaerobic culture for 48h;

[0041] Step 4: Take the bacterial solution and centrifuge at 6000rpm for 10min. Wash twice with normal saline, and finally reconstitute the sludge with normal saline for use and count the viable bacteria.

Embodiment 2

[0042] Example 2: Mouse experiments on the therapeutic effect of Bacteroides fragilis on Mycobacterium tuberculosis infection

[0043] figure 1 Schematic diagram of the experimental flow for testing the therapeutic effect of Bacteroides fragilis and inactivated Bacteroides fragilis on Mycobacterium tuberculosis infection.

[0044] figure 2 Schematic diagram of the experimental flow for detecting the preventive effect of B. fragilis and inactivated B. fragilis on Mycobacterium tuberculosis infection.

[0045] 1. Training method

[0046] The culture method of Bacteroides fragilis is the same as in Example 1.

[0047] 2. Sample preparation

[0048] 1) Preparation of live Bacteroides fragilis ZY-312 cells

[0049] Step 1: Take one freeze-dried strain, add 200 freeze-dried strain culture medium, redissolve, absorb 20 bases, line the blood plate, and put it in the biochemical incubator for 37 plates after the gas control system of the anaerobic tank is pumped , Anaerobic cult...

Embodiment 3

[0065] Example 3: Protective efficacy of tuberculosis BCG based on Bacteroides fragilis adjuvant

[0066] To assess the ability of B. fragilis-based adjuvants to provide protection against tuberculosis infection caused by Mycobacterium tuberculosis infection, BCG was performed on C57BL / 6 mice using live and inactivated B. fragilis and control PBS. Immunization.

[0067] Two weeks after the last vaccination, mice were challenged with live M. tuberculosis by intraperitoneal route. The ability of the vaccine to reduce M. tuberculosis load was measured in the lung after 4 weeks. These data show a significant level of protection using Bacteroides fragilis and its inactivated strains as adjuvants ( Figure 4 ).

[0068] It can be seen from the above results that the Bacteroides fragilis of the present invention has a good effect on the prevention and / or treatment of tuberculosis caused by Mycobacterium tuberculosis infection, and is safe and non-toxic, providing a new option for ...

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Abstract

The invention belongs to the biomedical field, in particular to the use of Bacteroides fragilis in the preparation of drugs for the treatment and prevention of tuberculosis. The invention also provides the application of Bacteroides fragilis in the preparation of pharmaceutical compositions, foods, health products and food additives for treating and / or preventing tuberculosis. The Bacteroides fragilis provided by the invention can effectively treat and / or prevent tuberculosis and enhance the immune protection effect of tuberculosis bacillus Calmette-Guerin vaccine (BCG), and has wide application prospect.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of Bacteroides fragilis in the preparation of medicines for preventing and / or treating tuberculosis. Background technique [0002] Tuberculosis (TB) caused by Mycobacterium tuberculosis (M.tb) infection is one of the severe infectious diseases with the highest infection rate, morbidity rate and fatality rate. One of the public health security threats. The latest global tuberculosis epidemic annual report released by the World Health Organization (WHO) in 2016 shows that in 2016, there were more than 9 million new cases of tuberculosis, and the number of deaths from tuberculosis reached 2 million, which has exceeded the number of deaths caused by HIV / AIDS infection; and, my country's tuberculosis epidemic and Mycobacterium tuberculosis infection are quite serious, and it is one of the 22 countries with a high burden of tuberculosis in the world, and the number of tubercu...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61P31/06A23L33/135A23L29/00
CPCA23V2002/00A61K35/741A23L29/065A23L33/135A23V2200/314
Inventor 曾谷成
Owner REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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