Application of TREM-2+T cells in preparation of drugs for treating tuberculosis

A tuberculosis and cell technology, which is applied in the application field of TREM-2+T cells in the preparation of drugs for the treatment of tuberculosis, can solve problems such as drug resistance of Mycobacterium tuberculosis, reduce inflammation, reduce pulmonary Mycobacterium tuberculosis, Scientific and efficient preparation process

Active Publication Date: 2020-05-22
THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, long-term chemotherapy can easily lead to drug resistance of Mycobacterium tuberculosis

Method used

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  • Application of TREM-2+T cells in preparation of drugs for treating tuberculosis
  • Application of TREM-2+T cells in preparation of drugs for treating tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Experiment preparation:

[0022] (1) Specific fluorescent antibody to TREM-2; (2) Specific fluorescent antibody to T cells; (3) 1640 medium with 10% fetal bovine serum; (4) Interleukin-2; (5) Mycobacterium tuberculosis infection Recipient mice; (6) TREM-2-T cells (ie negative T cells).

[0023] Experimental steps:

[0024] (1) Take 20ml of peripheral blood, and use lymphocyte separation medium to separate peripheral blood mononuclear cells;

[0025] (2) Staining and marking TREM-2+ T cells;

[0026] (3) Sorting and purifying TREM-2+ T cells by flow cytometry;

[0027] (4) Add medium and interleukin-2 (20ng / ml) to culture in vitro for 14 days, and change the medium and interleukin-2 every 3 days;

[0028] (5) The cells were washed with normal saline for 3 times and injected intravenously into recipient mice.

[0029] (6) Judgment of curative effect: With no injection of cells and injection of TREM-2-T cells (i.e. negative T cells) as controls, the number of Mycobact...

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Abstract

The present invention relates to the technical field of bio-pharmaceuticals, and particularly relates to an application of TREM-2+T cells in preparation of drugs for treating tuberculosis. The application of TREM-2+T cells has the following beneficial effects: the TREM-2+T cells are applied to prepare drugs treating tuberculosis for the first time, and the drugs can achieve immunotherapy on the tuberculosis, relieve pulmonary tuberculosis bacteria and reduce inflammation. In addition, since the TREM-2+T cells are derived from the autologous peripheral blood of a patient without rejection, thereaction is suitable for comprehensive treatment of tuberculosis. According to the invention, the TREM-2+T cells for treating the tuberculosis are prepared by processes such as flow cytometric sorting, in-vitro culture amplification stimulated by interleukin 2 and washing, and the preparation method is more scientific and efficient.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to the application of TREM-2+T cells in the preparation of drugs for treating tuberculosis. Background technique [0002] Tuberculosis is a health and medical problem in our country, and the current clinical treatment is mainly through chemical drugs. However, long-term chemotherapy can easily lead to drug resistance of Mycobacterium tuberculosis. With the high rate of drug resistance and the spread of drug-resistant bacteria, the treatment of tuberculosis is becoming more and more severe. Therefore, there is an urgent need to develop new regimens to treat TB and drug-resistant TB. In recent years, many attempts have been made in the field of biotherapy, such as cellular immunotherapy, cytokines, monoclonal antibodies, etc. Among them, cell therapy is to activate and expand autologous or allogeneic immune effector cells in vitro, and then infuse them back to the patien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/17A61P31/06C12N5/0783
CPCA61K35/17A61K9/0019C12N5/0636A61P31/06C12N2501/2302
Inventor 黄曦吴永坚
Owner THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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