Application of TREM-2+T cells in preparation of drugs for treating tuberculosis
A tuberculosis and cell technology, which is applied in the application field of TREM-2+T cells in the preparation of drugs for the treatment of tuberculosis, can solve problems such as drug resistance of Mycobacterium tuberculosis, reduce inflammation, reduce pulmonary Mycobacterium tuberculosis, Scientific and efficient preparation process
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[0021] Experiment preparation:
[0022] (1) Specific fluorescent antibody to TREM-2; (2) Specific fluorescent antibody to T cells; (3) 1640 medium with 10% fetal bovine serum; (4) Interleukin-2; (5) Mycobacterium tuberculosis infection Recipient mice; (6) TREM-2-T cells (ie negative T cells).
[0023] Experimental steps:
[0024] (1) Take 20ml of peripheral blood, and use lymphocyte separation medium to separate peripheral blood mononuclear cells;
[0025] (2) Staining and marking TREM-2+ T cells;
[0026] (3) Sorting and purifying TREM-2+ T cells by flow cytometry;
[0027] (4) Add medium and interleukin-2 (20ng / ml) to culture in vitro for 14 days, and change the medium and interleukin-2 every 3 days;
[0028] (5) The cells were washed with normal saline for 3 times and injected intravenously into recipient mice.
[0029] (6) Judgment of curative effect: With no injection of cells and injection of TREM-2-T cells (i.e. negative T cells) as controls, the number of Mycobact...
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