Anti-tumor vaccine with core-shell structure containing branched polymer and mRNA and application of anti-tumor vaccine

A branched polymer, core-shell structure technology, applied in the field of biomedicine, can solve problems such as poor anti-tumor effect of vaccines

Pending Publication Date: 2021-01-22
上海杏禾医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] The object of the present invention is to provide an anti-tumor vaccine containing a branched polymer and a nucleocapsid structure of mRNA and its application, the anti-tumor

Method used

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  • Anti-tumor vaccine with core-shell structure containing branched polymer and mRNA and application of anti-tumor vaccine
  • Anti-tumor vaccine with core-shell structure containing branched polymer and mRNA and application of anti-tumor vaccine
  • Anti-tumor vaccine with core-shell structure containing branched polymer and mRNA and application of anti-tumor vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1: the construction of branched polymer macromolecules:

[0060] Dissolve 0.2 g of a multi-arm initiator (such as PAMAMG0) in dry 100 ml of dimethylformamide, add 10 g of benzyl aspartate cyclic anhydride and stir at room temperature for 48 hours, then use diethyl ether to precipitate the product. In order to obtain a branched polymer macromolecule with a cation in the side chain, the obtained branched polyamino acid can be deprotected. Specifically, 1 g of the branched polymer macromolecule is dissolved in 10 ml of dimethyl sulfoxide, and the Wherein dropwise add the diethylenetriamine of 50 times than side group, react at room temperature for 1 hour and use hydrochloric acid to adjust the pH value of the solution to 2.0, then dialyze with distilled water and lyophilize to obtain cationic branched polymer macromolecule (specific reaction The process and structure are shown below).

[0061]

Embodiment 2

[0062] Embodiment 2: the composite of branched polymer macromolecule and mRNA:

[0063] Agarose gel electrophoresis: the synthesized branched polymer macromolecule and the mRNA segment encoding ovalbumin (OVA) were dissolved in distilled water and mixed according to different nitrogen-phosphorus ratios and stabilized at room temperature for 15 minutes ( figure 1 A), then the complex was added to an agarose gel with a mass percent concentration of 2% and operated at 80V for 30 minutes, and then the gel was stained with ethidium bromide and analyzed using ChemDoc TM It was imaged with the MP gel imaging system (Bio-Rad). The result is as figure 2 As shown in A, when the nitrogen to phosphorus ratio is greater than 1, the mRNA is completely encapsulated by the branched polymer macromolecules.

[0064] Particle size potential: The synthesized branched polymer macromolecules and mRNA were dissolved in distilled water and mixed according to different nitrogen to phosphorus ratio...

Embodiment 3

[0069] Example 3: Evaluation of nucleocapsid structure on mRNA encapsulation efficiency and in vitro toxicity:

[0070] Disperse the composite nanoparticles of branched polymer macromolecules prepared according to different nitrogen-phosphorus ratios and mRNA of known concentration in RNase-free water to make solution A, set the volume to 0.1ml to 10ml, and the mRNA concentration is 1μg / ml to 100 μg / ml; the above optimal ratio of lipid molecules such as (2,3-dioleoyl-propyl)-trimethylamine, dipalmitoylphosphatidylcholine and cholesterol in ethanol solution 40:20:40 (wt: wt) to prepare solution B, and finally adjust the volume to 0.1ml to 10ml. Using Nanoassemblr TM Mix solution A with solution B at a mixing flow rate from 0.1ml / min to 10ml / min. After preparation, the filtrate was collected by ultrafiltration and centrifugation, and the residual mRNA in the filtrate was determined using the Ribo Green kit. The calculation formula for the encapsulation efficiency of mRNA is as...

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Abstract

The invention provides an anti-tumor vaccine with a core-shell structure containing a branched polymer and mRNA. The anti-tumor vaccine comprises one mRNA chain segment for at least encoding a tumor antigen and at least one positively charged branched polymer macromolecule, and the mRNA and the branched polymer macromolecules are encapsulated in a biocompatible lipid double-layer shell after beingcompounded. According to the anti-tumor vaccine, the nano core-shell structure is constructed by multiple components such as the branched polymer macromolecules, lipid molecules, immune additives andthe like to deliver the mRNA for encoding the clinical tumor antigen so as to realize immunotherapy of tumors. The immune additives and the encoded mRNA are simultaneously integrated into a deliveryvaccine and the vaccine is efficiently delivered to mammalian cells for use in the treatment or amelioration of symptoms of one or more cancers in a mammal. The anti-tumor vaccine has the advantages of simple preparation method, wide anti-tumor spectrum, strong specificity and high efficiency.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a vaccine, in particular to an anti-tumor vaccine with a nucleocapsid structure containing a branched polymer and mRNA and its application. Background technique [0002] Vaccines usually consist of one or more antigens that can be recognized by the body's immune system and several adjuvants. Through the stimulation and activation of the body's immune system, the body's immune response to specific diseases can be enhanced, thereby effectively inhibiting the disease. occurrence, development and recurrence. Vaccines generally fall into two categories: preventive vaccines and therapeutic vaccines. Among them, therapeutic vaccines have shown great potential in targeting many types of life-threatening diseases including cancer and acute infectious diseases. [0003] However, vaccines against cancer are completely different from vaccines against infectious diseases that produce anti-pathogens,...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K9/51A61K47/34A61K45/06A61P35/00
CPCA61K39/0011A61K9/5146A61K45/06A61P35/00A61K2039/5154A61K2300/00
Inventor 齐若谷杨溪左青松丁海童
Owner 上海杏禾医疗科技有限公司
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