Drug-loaded microspheres, anti-tumor drug and preparation method

A technology of anti-tumor drugs and drug-loaded microspheres, which is applied in the field of tumor treatment, can solve the problems of irreversible tumor immune escape, failure to improve tumor immunosuppressive microenvironment, and poor tumor efficacy, so as to reduce tumor immune escape and promote tumor growth. Internal migration, effect of stabilizing local concentration

Inactive Publication Date: 2020-04-17
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Inside the tumor, tumor cells and stromal cells can secrete a variety of chemokines, but the function of most chemokines is to promote the migration of immunosuppressive cells into the tumor, while few chemokines can promote the migration of immune effector cells, leading to the killing of tumor cells. Tumor-killing immune effecto

Method used

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  • Drug-loaded microspheres, anti-tumor drug and preparation method
  • Drug-loaded microspheres, anti-tumor drug and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This example provides a preparation method of drug-loaded microspheres. Purchase the loaded matrix (Callispher, 100-300 μm, Hengrui Medicine) and CXCL10 from the market, take 1×10 4 A loading matrix was mixed with 0.1ug CXCL10, and the loading was allowed to stand for 30 minutes to obtain drug-loaded microspheres loaded with CXCL10.

experiment example 1

[0046] In this experimental example, the drug-loaded microspheres prepared in Example 1 were used to treat colon cancer in mice. Specifically include the following steps:

[0047] (1) Cell culture and passage: The mouse CT26 colon cancer cell line used in the experiment was routinely cultured in RPMI 1640 medium containing 10% inactivated fetal bovine serum, placed at 37°C, 5% CO 2 Cultured in an incubator, changing the medium every other day. Cells were digested with 0.15% trypsin [containing 0.02% ethylenediaminetetraacetic acid (EDTA)] prepared in phosphate buffered saline (PBS) and passaged every 3 days, and the cells in the logarithmic growth phase were collected for experiment.

[0048] (2) Establishment of mouse tumor model: collect cells in logarithmic growth phase, adjust cell concentration to 2.5×10 6 pieces / ml. The back skin of BALB / C mice was prepared, and 0.2ml of cell suspension (5×10 5 cells / side), observe the tumor formation every day, and consider the for...

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Abstract

The invention discloses drug-loaded microspheres, an anti-tumor drug and a preparation method, and relates to the technical field of tumor treatment. The drug-loaded microspheres comprise a loading matrix and an active factor, and the active factor is adsorbed on the loading matrix; wherein the active factor comprises any one or a combination of a chemotactic factor and a cytokine. The drug-loadedmicrospheres can chemotactic the transfer of tumor cells and immune effector cells through loading the loading matrix with the chemotactic factor, promote immune effector cells to kill tumor cells ina targeted manner; and the loading matrix loaded cytokine can promote the immune effector cells to migrate into tumors and promote the proliferation or survival of the immune effector cells, therebyrealizing tumor immunotherapy. The preparation method of the drug-loaded microspheres is simple and easy to popularize and apply. The anti-tumor drug can be prepared from the drug-loaded microspheresand act on tumor cells in a targeting manner.

Description

technical field [0001] The invention relates to the technical field of tumor treatment, in particular to a drug-loaded microsphere, an antitumor drug and a preparation method. Background technique [0002] Immunotherapy is the most promising tumor treatment strategy for clinical application. The basic principle of tumor immunotherapy is to control tumors by enhancing the function of autoimmune effector cells or adopting anti-tumor active cells through biological methods. For solid tumors, an important part of the efficacy of tumor immunotherapy is the migration of immune effector cells into the tumor to achieve direct contact with tumor cells, thereby killing tumor cells. [0003] Inside the tumor, tumor cells and stromal cells can secrete a variety of chemokines, but the function of most chemokines is to promote the migration of immunosuppressive cells into the tumor, while few chemokines can promote the migration of immune effector cells, leading to the killing of tumor c...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K47/36A61K47/38A61K47/34A61K47/32A61K38/19A61K45/06A61K38/20A61K38/21A61K38/18A61P35/00
CPCA61K9/5036A61K9/5042A61K9/5031A61K9/5026A61K38/195A61K45/06A61K38/2013A61K38/2046A61K38/21A61K38/191A61K38/18A61P35/00A61K2300/00
Inventor 石亮荣
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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