Application of trem-2+t cells in preparation of drugs for treating tuberculosis
A tuberculosis and cell technology, which is applied in the application field of TREM-2+T cells in the preparation of drugs for the treatment of tuberculosis, can solve problems such as drug resistance of Mycobacterium tuberculosis, reduce inflammation, reduce pulmonary Mycobacterium tuberculosis, Scientific and efficient preparation process
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[0021] Experiment preparation:
[0022] (1) TREM-2 specific fluorescent antibody; (2) T cell specific fluorescent antibody; (3) 1640 medium with 10% fetal bovine serum; (4) interleukin-2; (5) Mycobacterium tuberculosis infection Recipient mice; (6) TREM-2-T cells (ie negative T cells).
[0023] Experimental steps:
[0024] (1) 20ml of peripheral blood was drawn, and lymphocyte separation medium was used to separate peripheral blood mononuclear cells;
[0025] (2) Stain and label TREM-2+ T cells;
[0026] (3) Sorting and purifying TREM-2+ T cells by flow sorter;
[0027] (4) Add medium and interleukin-2 (20ng / ml) for 14 days in vitro, and change the medium and interleukin-2 every 3 days;
[0028] (5) The cells were washed 3 times with normal saline and injected intravenously into the recipient mice.
[0029] (6) Efficacy determination: with no cell injection and TREM-2-T cell injection (ie negative T cells) as the control, the number of Mycobacterium tuberculosis in the lu...
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