Application of trem-2+t cells in preparation of drugs for treating tuberculosis

A tuberculosis and cell technology, which is applied in the application field of TREM-2+T cells in the preparation of drugs for the treatment of tuberculosis, can solve problems such as drug resistance of Mycobacterium tuberculosis, reduce inflammation, reduce pulmonary Mycobacterium tuberculosis, Scientific and efficient preparation process

Active Publication Date: 2022-06-24
THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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  • Description
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AI Technical Summary

Problems solved by technology

However, long-term chemotherapy can easily lead to drug resistance of Mycobacterium tuberculosis

Method used

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  • Application of trem-2+t cells in preparation of drugs for treating tuberculosis
  • Application of trem-2+t cells in preparation of drugs for treating tuberculosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Experiment preparation:

[0022] (1) TREM-2 specific fluorescent antibody; (2) T cell specific fluorescent antibody; (3) 1640 medium with 10% fetal bovine serum; (4) interleukin-2; (5) Mycobacterium tuberculosis infection Recipient mice; (6) TREM-2-T cells (ie negative T cells).

[0023] Experimental steps:

[0024] (1) 20ml of peripheral blood was drawn, and lymphocyte separation medium was used to separate peripheral blood mononuclear cells;

[0025] (2) Stain and label TREM-2+ T cells;

[0026] (3) Sorting and purifying TREM-2+ T cells by flow sorter;

[0027] (4) Add medium and interleukin-2 (20ng / ml) for 14 days in vitro, and change the medium and interleukin-2 every 3 days;

[0028] (5) The cells were washed 3 times with normal saline and injected intravenously into the recipient mice.

[0029] (6) Efficacy determination: with no cell injection and TREM-2-T cell injection (ie negative T cells) as the control, the number of Mycobacterium tuberculosis in the lu...

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Abstract

The present invention relates to the technical field of biopharmaceuticals, in particular to the application of TREM-2+T cells in the preparation of drugs for treating tuberculosis. The drug can realize immunotherapy for tuberculosis, reduce pulmonary tuberculosis, and reduce inflammation; in addition, because TREM-2+ T cells are derived from the patient's autologous peripheral blood, there is no rejection, and the reaction is suitable for comprehensive treatment of tuberculosis; TREM-2+ T cells for the treatment of tuberculosis are prepared through the process of cell sorting, interleukin-2 stimulation in vitro culture expansion and washing, and the preparation process is more scientific and efficient.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to the application of TREM-2+T cells in the preparation of medicines for treating tuberculosis. Background technique [0002] Tuberculosis is a health and medical problem in my country, and the current clinical treatment is mainly through chemical drugs. However, long-term chemotherapy can easily lead to drug resistance of Mycobacterium tuberculosis. With high drug resistance rates and the spread of drug-resistant bacteria, the treatment of tuberculosis is becoming more and more severe. Therefore, there is an urgent need to develop new treatments for TB and drug-resistant TB. In recent years, many attempts have been made in the field of biotherapy, such as cellular immunotherapy, cytokines, and monoclonal antibodies. Among them, cell therapy is to activate and amplify autologous or allogeneic immune effector cells in vitro, and then infuse them back to the patient to k...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783A61K35/17A61P31/06
CPCA61K35/17A61K9/0019C12N5/0636A61P31/06C12N2501/2302
Inventor 黄曦吴永坚
Owner THE FIFTH AFFILIATED HOSPITAL SUN YAT SEN UNIV
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