Catechu extract composition for resisting tubercle bacillus, preparation method of catechu extract composition, pharmaceutical preparation containing catechu extract composition, and application of catechu extract composition

Abstract

The invention discloses a catechu extract composition for resisting tubercle bacillus, a preparation method of the catechu extract composition, a pharmaceutical preparation containing the catechu extract composition, and application of the catechu extract composition. The catechu extract composition contains a flavanoid compound of which the purity is more than 50%, and the flavanoid compound takes catechin and / or epicatechin as main components. The main structural formulas (I) and (II) of the flavanoid compound are shown in the specifications, wherein R1, R2, R3, R4 and R5 are respectively and independently selected from hydrogen, C1-C6 alkyl, beta-D-glucopyranesyl, SO3 or PO3. By adopting a chromatographic process for refining and purifying the catechu extract composition disclosed by the invention, the effective content is as high as 99.5%. The catechu extract composition and the pharmaceutical composition thereof have excellent effects on improving or treating pulmonary tuberculosis, treating ulcerative bone tuberculosis and AIDS (Acquired Immune Deficiency Syndrome) complicated by tuberculosis, treating drug resistant tuberculosis, and especially treating drug resistant tuberculosis and AIDS complicated by tuberculosis.

Description

Technical field

[0001] The invention relates to the technical field of natural medicines, in particular to a catechin extract composition against tuberculosis bacillus, a preparation method thereof, and a pharmaceutical preparation and application containing them. Background technique

[0002] Catechu (catechu) is a dry extract made from dried branches of the leguminous Acacia catechu tree [Acacia catechu (L.) Willd.] and decoction in water. It is recorded in ancient books such as "Drinking and Diet Zheng Yao", "Compendium of Materia Medica", "Materia Medica Seeking Truth", "Materia Medica Zhengyi" and the calendar "Chinese Pharmacopoeia". Various sores, muscle growth and pain relief, hemostatic and dampening effects. It is used for the treatment of ulcers, eczema, aphthous, pain, traumatic bleeding and other diseases.

[0003] Modern research on catechin shows that it mainly contains catechin tannin, catechin, epicatechin, ochre tannin, fisetin, quercetin, quercetin marigold, pr...

Examples

Embodiment 1

[0031] 1. Extraction: 20g of Chinese medicine catechu, crushed and soaked in 50% ethanol for 3 times, 24 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat to dissolve, and filter to obtain a clear filtrate.

[0032] 2. Refining: The above-mentioned filtrate is eluted with a gradient of water and 10%, 20%, 30%, 40%, 50%, 75% ethanol through the treated HPD-100 type macroporous adsorption resin, HPLC detection, collecting the rich The fractions of catechin and epicatechin derivatives are combined, concentrated under reduced pressure, and freeze-dried to obtain 9.9 g of extract. According to the standard method of "The Pharmacopoeia of the People's Republic of China", the total content of the main active ingredients including catechin and epicatechin is 53.2% after HPLC external standard method determination. Catechin and epicatechin were identified by HPLC method and chemical reference substance. For details, see...

Embodiment 2

[0034] 1. Extraction: 20g of Chinese medicine catechu, crushed and added 50% ethanol, soaked at 50°C for 3 times, 6 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat to dissolve, and filter to obtain a clear filtrate.

[0035] 2. Refining: The above-mentioned filtrate is eluted with a gradient of water and 10%, 20%, 30%, 40%, 50%, and 75% ethanol through the treated HP-20 type macroporous adsorption resin, and HPLC detects and collects The fractions of catechin and epicatechin derivatives are combined, concentrated under reduced pressure, and freeze-dried to obtain 8.4 g of extract. According to the standard method of "The Pharmacopoeia of the People's Republic of China" and measured by the high performance liquid phase external standard method, the main active ingredients include catechin and epicatechin with a total content of 55.6%.

Embodiment 3

[0037] 1. Extraction: 20g of Chinese medicine catechu, crushed and soaked in 50% ethanol overnight, heated and refluxed for extraction 3 times, each for 3.0 hours. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat to dissolve, and filter to obtain a clear filtrate.

[0038] 2. Refining: The above-mentioned filtrate is passed through the treated HP-20 type macroporous adsorption resin, washed with water, 10%, 20%, 30%, 40%, 50% and 75% methanol in successive gradients, detected by HPLC, and collected The fractions containing catechin and epicatechin derivatives are combined, concentrated under reduced pressure, and freeze-dried to obtain 9.2 g of plant extract. According to the standard method of "The Pharmacopoeia of the People's Republic of China", the main active ingredients include 54.4% of the total content of catechin and epicatechin through high-performance liquid phase external standard method.

Technical field

[0001] The invention relates to the technical field of natural medicines, in particular to a catechin extract composition against tuberculosis bacillus, a preparation method thereof, and a pharmaceutical preparation and application containing them. Background technique

[0002] Catechu (catechu) is a dry extract made from dried branches of the leguminous Acacia catechu tree [Acacia catechu (L.) Willd.] and decoction in water. It is recorded in ancient books such as "Drinking and Diet Zheng Yao", "Compendium of Materia Medica", "Materia Medica Seeking Truth", "Materia Medica Zhengyi" and the calendar "Chinese Pharmacopoeia". Various sores, muscle growth and pain relief, hemostatic and dampening effects. It is used for the treatment of ulcers, eczema, aphthous, pain, traumatic bleeding and other diseases.

[0003] Modern research on catechin shows that it mainly contains catechin tannin, catechin, epicatechin, ochre tannin, fisetin, quercetin, quercetin marigold, pr...

Examples

Embodiment 1

[0031] 1. Extraction: 20g of Chinese medicine catechu, crushed and soaked in 50% ethanol for 3 times, 24 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat to dissolve, and filter to obtain a clear filtrate.

[0032] 2. Refining: The above-mentioned filtrate is eluted with a gradient of water and 10%, 20%, 30%, 40%, 50%, 75% ethanol through the treated HPD-100 type macroporous adsorption resin, HPLC detection, collecting the rich The fractions of catechin and epicatechin derivatives are combined, concentrated under reduced pressure, and freeze-dried to obtain 9.9 g of extract. According to the standard method of "The Pharmacopoeia of the People's Republic of China", the total content of the main active ingredients including catechin and epicatechin is 53.2% after HPLC external standard method determination. Catechin and epicatechin were identified by HPLC method and chemical reference substance. For details, see...

Embodiment 2

[0034] 1. Extraction: 20g of Chinese medicine catechu, crushed and added 50% ethanol, soaked at 50°C for 3 times, 6 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat to dissolve, and filter to obtain a clear filtrate.

[0035] 2. Refining: The above-mentioned filtrate is eluted with a gradient of water and 10%, 20%, 30%, 40%, 50%, and 75% ethanol through the treated HP-20 type macroporous adsorption resin, and HPLC detects and collects The fractions of catechin and epicatechin derivatives are combined, concentrated under reduced pressure, and freeze-dried to obtain 8.4 g of extract. According to the standard method of "The Pharmacopoeia of the People's Republic of China" and measured by the high performance liquid phase external standard method, the main active ingredients include catechin and epicatechin with a total content of 55.6%.

Embodiment 3

[0037] 1. Extraction: 20g of Chinese medicine catechu, crushed and soaked in 50% ethanol overnight, heated and refluxed for extraction 3 times, each for 3.0 hours. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat to dissolve, and filter to obtain a clear filtrate.

[0038] 2. Refining: The above-mentioned filtrate is passed through the treated HP-20 type macroporous adsorption resin, washed with water, 10%, 20%, 30%, 40%, 50% and 75% methanol in successive gradients, detected by HPLC, and collected The fractions containing catechin and epicatechin derivatives are combined, concentrated under reduced pressure, and freeze-dried to obtain 9.2 g of plant extract. According to the standard method of "The Pharmacopoeia of the People's Republic of China", the main active ingredients include 54.4% of the total content of catechin and epicatechin through high-performance liquid phase external standard method.

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