Gene chip and kit for detecting common pathogenic bacteria in food

A gene chip and pathogenic bacteria technology, applied in the field of gene chips and kits for detecting seven common pathogenic bacteria in food, can solve the problems of time-consuming, cumbersome, low detection sensitivity, etc., and achieve simple operation, strong repeatability, The effect of high accuracy
CN101967510BInactive Publication Date: 2012-10-17TIANJIN BIOCHIP TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TIANJIN BIOCHIP TECH CO LTD
Publication Date
2012-10-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a gene chip and a kit for detecting common pathogenic bacteria in food. The gene chip comprises a solid phase carrier and an oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe comprises DNA sequences which are selected from an nuc gene of staphylococcus aureus, an speB gene of streptococcus pyogenes, an invA gene of salmonella, an ipaHgene of Shigela, a zpx gene of Enterobacter sakazakii, a 16S-23S intergenic region of Klebsiella pneumoniae, a toxR gene of Vibrio parahaemolyticus and 16SDNA of the seven bacteria. The kit comprisesthe gene chip. By utilizing the gene chip and the kit to detect the seven common pathogenic bacteria in food, the invention has the advantages of simple and convenient operation, high accuracy and good repeatability.
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Description

technical field

[0001] The invention relates to a gene chip and a test kit for detection, in particular to a gene chip and a test kit for detecting seven common pathogenic bacteria in food. Background technique

[0002] Foodborne diseases and food contamination are still important factors that endanger public health. In my country, there were 150 cases of food poisoning reported officially in 2000, with 6273 people poisoned and 150 dead. In 2003, there were 379 cases of food poisoning, 12,876 people were poisoned, and 323 people died. Among nearly 10,000 cases of food poisoning every year, except for accidents, most of them are caused by pathogenic microorganisms. For example, in Ningxia in 1999, an outbreak of food poisoning caused by Salmonella contaminated meat occurred, and thousands of people fell ill. Internationally, food poisoning caused by food microorganisms is also a very serious food safety problem. According to incomplete statistics, 75% of the food poisoning...

Claims

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