Construction method and application of inducible transgenic mouse cardiomyopathy animal model

A technology of transgenic mouse and construction method, applied in the field of transgenic, can solve the problems of difficult and accurate identification of myocarditis and cardiomyopathy, potential risk of virus, long modeling time, etc., so as to avoid modeling waiting period and low maintenance cost. , the effect of high modeling success rate
CN108018310BActive Publication Date: 2020-07-28SUZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SUZHOU UNIV
Publication Date
2020-07-28

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Abstract

The invention relates to an expression vector containing a calpain-1 gene. The expression vector comprises a Tet operon, an alpha-MHC promoter nucleotide sequence, a mitochondrial signal peptide sequence and a gene segment of calpain-1 which are connected in sequence. The invention further provides a construction method of an inducible transgenic mouse cardiomyopathy animal model. The constructionmethod comprises the following steps: importing the expression vector containing the calpain-1 gene into oosperms of a mouse, and transferring the oosperms into the uterus of the receptor mouse for gestation, thus obtaining the positive offspring transgenic mouse; and mating the positive offspring transgenic mouse with a transgenic mouse with specific expression of tTA or a transgenic mouse withspecific expression of rtTA, and picking out the inducible transgenic mouse cardiomyopathy animal model from the hybrid offsprings. The invention further discloses applications of the transgenic mousecardiomyopathy animal model obtained through the construction method in screening medicines for treating cardiomyopathy. According to the technical scheme provided by the invention, the modeling success rate is high, the phenotype is stable, the cost is ow, and whether inducting for the generation of cardiomyopathy is needed or not can be selected according to the actual demands.
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Description

technical field

[0001] The invention relates to the field of transgenic technology, in particular to a construction method and application of an inducible transgenic mouse cardiomyopathy animal model. Background technique

[0002] Cardiomyopathy is a kind of myocardial disease caused by various reasons, characterized by myocardial enlargement, thickening and hardening. It is often manifested clinically as cardiac mechanical / electrical activity disturbance and abnormal hypertrophy or dilation of the ventricle. With the development of the disease, it can eventually evolve into heart failure. In recent years, the incidence of cardiomyopathy has been increasing year by year in the world, and it has become one of the most common clinical heart diseases in my country. The pathogenesis of cardiomyopathy is complex, the course of the disease is hidden, the prognosis is extremely poor, and there is a lack of specific treatment methods. It seriously endangers the health of the popula...

Claims

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