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Organoid technology-based individualized lung cancer primary cell culture method

A technology of primary cells and organoids, which is applied in the field of individualized lung cancer primary cell culture based on organoid technology, can solve the problems of inaccurate prediction of patients' clinical response to drugs and inability to predict the curative effect, and achieve high clinical relevance , not easy to mutate, high success rate effect

Inactive Publication Date: 2019-01-08
BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

However, due to the heterogeneity of tumors and the individual differences of patients, simple gene sequencing often cannot accurately predict the clinical response of patients to drugs in complex diseases such as tumors.
And gene sequencing cannot predict the curative effect of chemotherapy, traditional Chinese medicine and other treatments

Method used

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  • Organoid technology-based individualized lung cancer primary cell culture method
  • Organoid technology-based individualized lung cancer primary cell culture method
  • Organoid technology-based individualized lung cancer primary cell culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Organoid Culture

[0020] 100 tissue samples obtained from the operation were quickly transported to the laboratory for single-cell and three-dimensional culture. Through culturing, a bank of lung cancer specimens was established. Through ARMS-PCR technology, it was proved that the gene mutation carried by the organoid model was the same as that of the tumor tissue. Through the identification of short tandem repeats (short tandem repeats, STR), it was proved that the short tandem repeat sequences from the tumor tissue DNA were completely consistent with the short tandem repeat sequences from the organoid model DNA, and the establishment of the organoid model was confirmed through verification.

[0021] Specimen source:

[0022] (1) Inclusion criteria for patients with specimen source:

[0023] 1) Tissue sample washing solution (colorless).

[0024] 2) Tissue sample preservation solution (red). (stored at 4°C,)

[0025] 3) Disposable vacuum blood collecti...

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Abstract

The invention provides an organoid technology-based individualized lung cancer primary cell culture method. According to the method, high-throughput screening is carried out tumor cell models in vitrothrough organoid models established in tumor tissues of patients, so as to instruct individualized treatment of the patients. Specifically, excised lung cancer fresh tissues are utilized to carry outorganoid culture to establish individualized lung cancer primary cell models and carry out authentication, so that basis is provided for accurate treatment evaluation of lung cancer.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for culturing individualized lung cancer primary cells based on organoid technology. Background technique [0002] Lung cancer is a major disease that endangers the health of the Chinese people. The morbidity and mortality are as high as 53.37 / 100,000 and 45.57 / 100,000, respectively. The treatment of lung cancer is developing from evidence-based medicine to precision medicine, and the individualization of lung cancer treatment is also the general trend. Doctors formulate an "individualized" treatment plan with the best curative effect and the least toxicity according to the biological characteristic data of the patient's tumor. Maximize clinical benefit for patients. The treatment model for lung cancer under precision medicine will be individualized treatment based on clinical characteristics, pathological data, and biological molecular and cell characteristics. At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2509/00C12N2513/00
Inventor 韩毅刘志东曹小庆苏崇玉史抗程序李云峰
Owner BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
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