Morinda officinalis tissue culture method

A technology of tissue culture and Morinda officinalis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as poor genetic stability, easy variation, and quality decline, and achieve good genetic stability, no Easy to mutate and maintain the effect of medicinal properties

Inactive Publication Date: 2021-06-11
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the demand for Morinda officinalis has gradually increased; at present, Morinda officinalis is cultivated and planted mainly through asexual reproduction methods such as cutting propagation, which is simple and has a high survival rate, but asexual reproduction creates conditions for the accumulation of viruses, causing Germplasm degradation of Morinda officinalis, more diseases, lower yield and lower quality
[0003] In the related technology, in order to solve the above-mentioned problems, tissue culture of Morinda officinalis is carried out through indirect organogenesis, callus is induced by explants of Morinda officinalis, and adventitious buds are induced by callus to obtain tissue culture plantlets of Morinda officinalis; However, the method of obtaining the tissue culture seedlings of Morinda officinalis by inducing adventitious buds through callus has poor genetic stability and is prone to variation, which is not conducive to maintaining the specific traits of medicinal plants

Method used

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  • Morinda officinalis tissue culture method
  • Morinda officinalis tissue culture method
  • Morinda officinalis tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The detoxified and cleaned Morinda officinalis cane is used as explants, specifically, the explants are semi-lignified stem segments.

[0056] The explants were inoculated in the axillary bud induction medium, illuminated for 11 hours every day, the daily light intensity was 1300lx, the air temperature was 28°C, and the humidity was 75%. After 30 days of cultivation, the axillary buds grew. Among them, the axillary bud induction medium is 1 / 2MS+6-BA0.2mg / L+30g / L sucrose+4.3g / L agar powder, and the pH is 6.0.

[0057] The axillary buds were inoculated on the cluster bud induction medium, illuminated for 13 hours every day, the intensity of light every day was 1200 lx, the air temperature was 28° C., and the humidity was 75%. After 30 days of cultivation, the cluster buds grew. Wherein, the cluster bud induction medium is 1 / 2MS+6-BA0.2mg / L+30g / L sucrose+4.3g / L agar powder, and the pH is 6.0.

[0058] The cluster buds were inoculated on the rooting induction medium, illum...

Embodiment 2

[0060] For explant pretreatment, select adult 3-4-year-old Morinda officinalis, transplant it into a clean greenhouse, cut off the cane from the stem base of Morinda officinalis, and spray Morinda officinalis at a mass concentration of 10 every 7 days. % carbendazim solution, a new cane grows after one month as explant material.

[0061] The explants are disinfected and cleaned; the explants include young part, semi-lignified stem segment and fully lignified stem segment.

[0062] The explants were inoculated on the axillary bud induction medium, illuminated for 12 hours every day, the daily light intensity was 1200 lx, the air temperature was 28° C., and the humidity was 75%. After 30 days of cultivation, the axillary buds grew. Among them, the axillary bud induction medium is 1 / 2MS+6-BA0.2mg / L+30g / L sucrose+4.3g / L agar powder, and the pH is 6.0.

[0063] The axillary buds were inoculated on the bud-clump induction medium, illuminated for 12 hours every day, the intensity of...

Embodiment 3

[0066] For explant pretreatment, select adult 3-4-year-old Morinda officinalis, transplant it into a clean greenhouse, cut off the cane from the stem base of Morinda officinalis, and spray Morinda officinalis at a mass concentration of 10 every 7 days. % carbendazim solution, grow new cane after one month as explant material (such as figure 1 shown).

[0067] The newly grown cane explant material is placed on the ultra-clean workbench, the surface of the explant material is wiped with 75% alcohol, and cut into stem segments with a length of 2-3cm with nodes, wherein, cut Include the young tender stem section within 20cm from the top, the semi-lignified stem section approximately 20-40cm away from the top and the fully lignified stem section approximately more than 40cm from the top in the divided stem section. Disinfect young stems with 0.1% mercuric chloride solution for 4min30s, and sterilize semi-lignified and fully lignified stems with 0.1% mercuric chloride solution for ...

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Abstract

The invention relates to the technical field of tissue culture, in particular to a morinda officinalis tissue culture method. The method comprises the following steps of taking a disinfected and cleaned morinda officinalis rattan as an explant; inoculating the explant to an axillary bud induction culture medium, and illuminating the explant every day until axillary buds grow out; inoculating the axillary buds to a cluster bud induction culture medium, and illuminating the axillary buds every day until cluster buds grow out; and inoculating the cluster buds to a rooting induction culture medium, and illuminating the cluster buds every day until root systems grow out. According to the morinda officinalis tissue culture method, the rattan of the morinda officinalis is adopted as the explant for tissue culture, namely, a direct organogenesis way of the morinda officinalis is used, a callus stage is not needed, good hereditary stability is achieved, variation is not prone to occurring, and the specific medicinal characters of the morinda officinalis can be effectively kept.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture of Morinda officinalis. Background technique [0002] Morinda officinalis How is a Rubiaceae plant and one of the famous "Four Southern Medicines". Its dried roots are used as medicine, which has the effects of strengthening muscles and bones, nourishing kidney yang, and expelling wind and dampness. At the same time, Morinda officinalis also has a wide range of health benefits, and can be used in large quantities in preserved fruits, soups, ingredients, etc. In recent years, the demand for Morinda officinalis has gradually increased; at present, Morinda officinalis is cultivated and planted mainly through asexual reproduction methods such as cutting propagation, which is simple and has a high survival rate, but asexual reproduction creates conditions for the accumulation of viruses, causing The germplasm of Morinda officinalis is degraded, wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 丁平冯冲
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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