Serum-free medium of adipose stem cells

A serum-free culture medium and human adipose stem cell technology, applied in the field of cell engineering, can solve problems such as insufficient cell number, and achieve the effects of high cell expansion multiples, insufficient cell number, and safety problems

Inactive Publication Date: 2019-02-15
YOCON BIOLOGY TECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention can solve the problem of insufficient cell quantity, and the cell quantity, survival rate and immune phenotype of each batch are more stable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Preparation of human adipose stem cell serum-free medium

[0063] The concentration of each raw material is as follows, and the unit of each concentration is mg / L unless otherwise specified:

[0064] L-Arginine 210;

[0065] CuSO 4 ·5H 2 O 0.002;

[0066] L-asparagine 55;

[0067] L-aspartic acid 20;

[0068]L-glutamic acid 25;

[0069] Ni (NO 3 ) 2 ·6H 2 O 0.00012;

[0070] L-Glutamine 300;

[0071] ZnSO 4 ·7H 2 O 0.32;

[0072] Glycine 12;

[0073] CoCl 2 ·6H 2 O 0.0055;

[0074] L-histidine 15;

[0075] NaSiO 3 9H 2 O 0.006;

[0076] L-isoleucine 45;

[0077] Na 3 VO 4 12H 2 O 0.0015;

[0078] L-lysine hydrochloride 35;

[0079] SnCl 2 2H 2 O 0.000045;

[0080] L-methionine 20;

[0081] Na 2 SeO 3 0.006;

[0082] L-phenylalanine 18;

[0083] FeSO 4 ·7H 2 O 1.0;

[0084] L-proline 20;

[0085] Glucose 3000;

[0086] L-serine 25;

[0087] Vitamin C 0.45;

[0088] L-threonine 25;

[0089] Paraben 1.0;

[0090] L-try...

Embodiment 2

[0114] Example 2 Preparation of human adipose stem cell serum-free medium

[0115] The concentration of each raw material is as follows, and the unit of each concentration is mg / L unless otherwise specified:

[0116] L-Arginine 105;

[0117] CuSO 4 ·5H 2 O 0.0006;

[0118] L-asparagine 70;

[0119] L-aspartic acid 32;

[0120] L-glutamic acid 15;

[0121] Ni (NO 3 ) 2 ·6H 2 O 0.000032;

[0122] L-Glutamine 500;

[0123] ZnSO 4 ·7H 2 O 0.65;

[0124] Glycine 5;

[0125] CoCl 2 ·6H 2 O 0.001;

[0126] L-histidine 35;

[0127] NaSiO 3 9H 2 O 0.01;

[0128] L-Isoleucine 20;

[0129] Na 3 VO 4 12H 2 O 0.0005;

[0130] L-lysine hydrochloride 50;

[0131] SnCl 2 2H 2 O 0.0001;

[0132] L-methionine 15;

[0133] Na 2 SeO 3 0.002;

[0134] L-phenylalanine 25;

[0135] FeSO 4 ·7H 2 O 1.5;

[0136] L-proline 5;

[0137] Glucose 1000;

[0138] L-serine 40;

[0139] Vitamin C 0.7;

[0140] L-threonine 10;

[0141] p-Hydroxybenzoic acid 0.3;

[0...

Embodiment 3

[0166] Example 3 Preparation of human adipose stem cell serum-free medium

[0167] The concentration of each raw material is as follows, and the unit of each concentration is mg / L unless otherwise specified:

[0168] L-Arginine 305;

[0169] CuSO 4 ·5H 2 O 0.005;

[0170]L-asparagine 28;

[0171] L-Aspartate 12;

[0172] L-glutamic acid 35;

[0173] Ni (NO 3 ) 2 ·6H 2 O 0.0003;

[0174] L-glutamine 0;

[0175] ZnSO 4 ·7H 2 O 0.065;

[0176] Glycine 20;

[0177] CoCl 2 ·6H 2 O 0.008;

[0178] L-histidine 5;

[0179] NaSiO 3 9H 2 O 0.001;

[0180] L-isoleucine 75;

[0181] Na 3 VO 4 12H 2 O 0.005;

[0182] L-lysine hydrochloride 25;

[0183] SnCl 2 2H 2 O 0.00001;

[0184] L-methionine 35;

[0185] Na 2 SeO 3 0.01;

[0186] L-phenylalanine 5;

[0187] FeSO 4 ·7H 2 O 0.1;

[0188] L-proline 30;

[0189] Glucose 4000;

[0190] L-serine 10;

[0191] Vitamin C 0.19;

[0192] L-threonine 30;

[0193] p-Hydroxybenzoic acid 1.8;

[0194] L-t...

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PUM

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Abstract

The invention discloses a serum-free medium of human adipose stem cells. The serum-free medium comprises main components such as amino acids, inorganic salt components, a growth factor and albumin. The invention provides the serum-free medium of the human adipose stem cells, which does not contain animal-derived components and can completely realize serum-free culture of the stem cells. By adopting the serum-free medium provided by the invention to culture the human adipose stem cells, a large number of high-purity, safe and high-quality mesenchymal stem cells can be obtained in vitro; meanwhile, the cell survival rate can be increased, and the stem cell characteristics can be maintained. The serum-free medium can solve the problem of insufficient cells, and the cell quantity, survival rate and immunophenotype of each batch of the adipose stem cells are more stable.

Description

technical field [0001] The invention relates to a serum-free medium for human adipose stem cells, belonging to the technical field of cell engineering. Background technique [0002] Mesenchymal stem cells (MSCs) are derived from mesoderm, also known as multipotent mesenchymal stromal cells (multipotent mesenchymal stromal cells). The multilineage differentiation potential of germ layer cell differentiation has low immunogenicity, does not stimulate allogeneic immune response, is not killed by cytotoxic T cells and NK cells, and has immunomodulatory effects. Under the corresponding induction conditions, MSCs can differentiate into different types of cells such as bone, fat, cartilage, muscle and liver cells. Adipose stem cells (adipose derivedstem cslls, ADSC) is a kind of adult mesenchymal stem cells (mesenchymal stem cells, MSC) existing in adipose tissue. Under specific induction conditions, it can differentiate into different germ layer cells and tissues. In 2001, adip...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2500/90C12N2501/115C12N2500/12C12N2500/20C12N2500/22C12N2500/24C12N2500/25C12N2500/30C12N2500/34C12N2500/36C12N2500/44C12N2500/46C12N2500/32C12N2501/71C12N2501/998C12N2501/39C12N2501/15
Inventor 路明华冯成宝
Owner YOCON BIOLOGY TECH CO
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