51results about How to "Short doubling time" patented technology

The invention belongs to the field of marine microalgae cultivation, and relates to a formula of a Chaetoceros muelleri medium and a white plastic barrel aerated culture method. Chaetoceros muelleri is a small marine diatom, rich in polyunsaturated fatty acid and protein, and is a high-quality bait for raising sea treasures such as prawn and abalone. At present, an f / 2 culture medium formula used to produce Chaetoceros muelleri has problems of imbalanced and incomprehensive nutrients, slow growth, long multiplication time and low yield. ccording to experiments, nitrate is substituted by urea, which is used as a nitrogen source and supplemented by NaHCO3 and growth hormone, VB12 and VB1, so that the medium gains more comprehensive and balanced nutrients, and yield of Chaetoceros muelleri is increased by 87%. In addition, cement pool and glass cylinder barrel used for culture in current production have many disadvantages: easy pollution, difficult washing, inconvenient operation and easy cutting. Study in the invention shows that a white plastic barrel aerated culture method for culturing Chaetoceros muelleri has advantages of flexible operation, no pollution, easy washing, no cutting, low cost, high yield and large-scale promotion.

A hybrid multi-radionuclide sealed source for use in brachytherapy comprising a plurality of radionuclides is disclosed. The differing decay rates of the radionuclides in the hybrid multi-radionuclide sealed source combine a large initial dose of radiation followed by an extended dose of radiation contained within the single source. The sealed source may comprise a seed, a flexible strand, a rigid strand, a wire, a coil or a catheter.

The invention discloses a method for producing glutamic acid through double-feeding fermentation optimization of corn steep liquor and glucose, which comprises the following steps of: in fermentation culture of glutamic acid, initially adding 1-10g/L of glucose to a fermentation culture medium, and initially adding 1-10g/L of corn steep liquor; after the fermentation culture starts, starting feeding the corn steep liquor and glucose at the same time, wherein 5-19g/L of corn steep liquor is fed and completely added within 5-10 hours since the fermentation starts, and the total amount of the corn steep liquor is 15-20g/L; feeding the concentrated solution of glucose until the fermentation is over; maintaining the amount of residual sugar in the fermentation culture medium at 0.05-10g/L; and controlling the pH value of the liquid ammonia in the whole process of fermentation to 6.8-7.3. The method disclosed by the invention effectively increases the growth speed of thalli and shortens the adaptation period in the initial stage of thallus fermentation and the thallus doubling time, thus shortening the overall fermentation time and reducing the overall production cost of glutamic acid.

The invention belongs to the field of environmental engineering, which relates to an integrated expanded granular sludge bed-membrane bioreactor whole-course autotrophy denitrification device and a process thereof. The device comprises a reactor main body, an air/liquid separation tank, a three-phase separation device, a membrane assembly, an air pump, an air flow meter, a perforated aeration pipe, a water feeding pump, a reflux pump, a water discharging pump and the like, wherein the reactor main body is divided into two function regions, i.e., a lower whole-course autotrophy denitrification function region and an upper membrane filtration function region, wherein an air aeration mode is adopted by the bottom of the immersion-type membrane assembly in the upper membrane filtration function region; the upper part of the reactor is externally connected with the air/liquid separation tank; the reflux system is used for refluxing heavy-oxygen-enriched water in the tank to a water inlet in the bottom of the reactor so as to provide the necessary dissolved oxygen for the whole-course autotrophy denitrification function region; and the fed sewage is firstly denitrified by the whole-course autotrophy denitrification function region and then filtered by the membrane so as to obtain the clean discharging water. The integrated reactor is small in land occupation. The whole-course autotrophy denitrification process is simple and convenient to operate.

The invention relates to the field of cell culture medium, and in particular to a non-serum non-animal-origin-additive insect cell culture medium. The medium comprises mainly the following components: basic culture medium, glucose, inorganic salt, vitamins, L-arginine, L-agedoite, L-glycocoll, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-threonine, L-tryptophan, L-valine, L-proline, L-glutamine, yeast extracts, recombulin, malic acid, allomaleic acid, cholesterol, linoleic acid, granulesten, putrescine, glutathione, glycerol and fructosan. The culture medium can promote the growth of insect cell and is suitable for the large scale breeding of insect cell and the expression of recombination protein.

The invention provides a serum-free culture medium system for culture of primary human epidermic cells, relates to the technical field of biological medical materials, and discloses application of a serum-free culture medium in in-vitro culture of human epidermic cells. The serum-free culture medium system has the advantage that the cholera toxin and various anchoring factors are not added, the potential hidden hazard due to complicated components is avoided, the epidermic cells can well grow, and the requirement of scaled production and application is met. The serum-free culture medium system for culture of primary human epidermic cells comprises a basic culture medium and exogenous adding factors, wherein the basic culture medium is formed by mixing a DMEM (dulbecco's modified eagle medium) commercial culture medium and a F12 commercial culture medium according to a certain ratio, and can also adopt a commercialized cell culture medium MCDB153; the exogenous adding factors comprise L-glutamine, EGF (epidermic growth factors), BPE (bovine pituitary extract), insulin, penicillin-streptomycin, calcium chloride, transferrin, adenine, hydrocortisone, and sodium selenite.

The invention discloses a WAYNE293LVPRO cell adapted to a serum-free culture medium environment and application of the WAYNE293LVPRO cell. The human embryonic kidney cell WAYNE 293 is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 24, 2021, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the postal code is 100101, and the preservation number is CGMCC No.22348. The human embryonic kidney cell WAYNE 293 provided by the invention is derived from suspension culture of HEK293 cells in a serum-free culture medium without an anti-caking agent, has the characteristics of short doubling time, high growth density, high virus packaging efficiency and the like, and is an important support for industrial development in the field of cell and gene therapy.

The invention discloses a hyaluronic acid method for promoting proliferation of human amniotic membrane stem cells and application thereof. The present invention applies hyaluronic acid in drugs for promoting proliferation of human amniotic stem cells. Through a series of tests, it is fully proved that hyaluronic acid can significantly reduce the doubling time of human amniotic stem cells (hASCs) and promote hASCs proliferation, has safety, and does not affect hASCs multi-differentiation potential of stem cells. Hyaluronic acid can be used alone, or a composition containing hyaluronic acid can be used for promoting the hASCs proliferation or manufacturing pharmaceuticals for the same purpose. The present invention has the advantages of wide range of sources, low cost, and side effects.

The invention relates to the fields of stem cells and tissue engineering, in particular to a method for extracting mesenchymal stem cells from the human umbilical cord. To solve the problems and defects in the conventional separation and culturing technology for human umbilical cord mesenchymal stem cells, the method adopts the technical scheme that the separation method for the human umbilical cord mesenchymal stem cells comprises the following steps: 1. preparation of an umbilical cord tissue block; 2. digestion of the tissue block; 3. filtration of a digestion solution, and centrifugal cell collection; 4. combination of cell sediments, centrifugation, and cell collection; 5. subculturing; 6. cell cryopreservation through a programmable cooler, and cryopreservation in liquid nitrogen. The method provided by the invention can lower the cost, achieve rapid obtaining of the mesenchymal stem cells, reduce cell damages, prolong the cryopreservation period, improve the cell activity, shorten the operational process, reduce pollution, and improve the cell purity.

The invention relates to the technical field of the wastewater biological treatment, and in particular relates to a device for fast screening and culturing anaerobic ammonia oxidation (Anammox) microbial communities. The device comprises an anaerobic reactor, an inner pressure tube type ultrafiltration membrane, a water inlet controlling device, an automatic pH regulating device, an equilibrium reagent-supplementing device, a pneumatic pressure regulation device and an electronic control automatic thermostatic device, wherein the anaerobic reactor is arranged in the electronic control automatic thermostatic device and connected with the inner pressure tube type ultrafiltration membrane, the automatic pH regulating device, the equilibrium reagent-supplementing device, the water inlet controlling device and the pneumatic pressure regulation device respectively. Compared with the prior art, the device for fast screening and culturing Anammox microbial communities can provide an environment for the rapid proliferation of Anammox, thus shortening the doubling time and increasing the growth rate of Anammox.

The invention relates to the technical field of biological pharmacy, in particular to development of a serum-free and animal-source-free culture medium additive suitable for insect cells mainly including Sf9 and High Five cells. A traditional culture medium Grace or IPL-41 serves as a basis, the additive beneficial for the growth of the insect cells is added, in the insect cell culture process, the additive has a function of replacing serum, price is low, the insect cells can grow for 240 h continuously, and cell activity can keep over 95%.

The invention relates to a platelet lysate supported micro-sphere preparation method, and aims to solve the problem of large platelet lysate consumption or poor micro-carrier adsorption effect in existing cell culture. The preparation method includes the steps of platelet lysate preparation, micro-sphere component preparation and platelet factor and micro-capsule supported micro-sphere preparation. A prepared micro-sphere is quite strong in osteoblast carrying capacity, most of cells are fitted on the micro-sphere under the condition that the ratio of osteoblasts to the micro-sphere is 500:1,and only few individual cells are scattered in culture solution. Mesenchymal stem cells on the micro-sphere are good in growth condition, cell viability exceeds 95%, doubling time is short, positive expression rate exceeds 95% in terms of purity, and the preparation method is applied to the technical field of biology.

The invention discloses a sheep stem extract and its preparation method and application, and discloses a preparation method of its preparation freeze-dried powder. The sheep stem extract is obtained by separating and culturing amniotic mesenchymal stem cells from human placenta amniotic tissue, and using human platelets The lysate is cultured and expanded, and then the culture supernatant of human amniotic mesenchymal stem cells is collected, concentrated and collected, which solves the problems of long doubling time and poor multi-differentiation potential of AMSCs, and can qualitatively and Quantitative analysis can improve the active ingredients in sheep stems, such as antioxidants and various growth factors, and prevent the active ingredients from degrading. The results of animal experiments and clinical trials show that the sheep dry extract prepared by this technology can be used to delay aging, prevent and treat osteoporosis and skin aging, and promote skin wound healing.

The invention provides a method for inducing and differentiating human embryonic stem cells (hESCs) into mesenchymal stem cells (MSCs). The method mainly relates to three stages, including induction of primitive streak (PS) cells, differentiation to outer mesoderm cells and differentiation of the MSCs. According to the method, a staged differentiation method is adopted, the whole differentiation process can be tracked and subjected to quality control, the cost is low, and operation is easy. An intermediate product of hESCs induced and differentiated into MSCs is obtained and comprises PS cells, outer mesoderm cells and MSCs, more MSCs can be efficiently generated, the multiplication time is shorter, the MSCs can be repeated, and mature MSCs can be obtained in 17 days at the shortest time,wherein the differentiation induction culture media in the first two stages are serum-free culture media with clear components, so that differentiated MSCs are prevented from being polluted by exogenous substances, and the uniformity and clinical applicability of the differentiated MSCs are guaranteed.

The invention relates to a method for promoting in-vitro expansion of human amniotic epithelial cells. The method comprises adding a nutritional composition during human amniotic epithelial cell culture, wherein the nutritional composition mainly comprises hyaluronic acid, an epidermal cell growth factor, vitamin C, a GlutaMAX-I additive, beta-mercaptoethanol, glycine, L-alanine, L-aspartic acid, L-asparagine, L-glutamic acid, L-proline and L-serine. The method can significantly promote human amniotic epithelial cell proliferation, reduce the doubling time, maintain biological characteristics such as human amniotic epithelial cell surface marker expression, multi-directional differentiation potential and immune tolerance, and significantly enhance the expression level of a human amniotic epithelial cell related gene and an immunosuppressive factor. Therefore, the nutritional composition as a human amniotic epithelial cell proliferation regulator has obvious advantages and has a great clinical significance to alleviate the shortage of seed cells in the field of regenerative medicine.

The invention provides a bioactive substance composition, a serum-free culture medium containing the composition and application of the serum-free culture medium. The bioactive substance composition is used for a serum-free culture medium and/or a composition and preparation thereof; the serum-free culture medium and/or composition can be used for primary culture and subculture of cells and/or tissues. The cells are selected from any one or more of tendon and/or ligament source cells, chondrocytes, meniscus stem cells, mesenchymal stem cells, skeletal stem cells and muscle stem cells. The tissue is a source tissue of a motor system. The bioactive substance composition and/or the serum-free culture medium and/or the composition can be used for preparing a medicine for treating tissue and/or organ injury; the tissue or organ injury is selected from movement system tissue or organ injury.

The invention discloses application of a human umbilical mesenchymal stem cell exosome in promotion of growth of mesenchymal stem cells of decidua parietalis and a method for promoting the growth of the mesenchymal stem cells of the decidua parietalis. According to the application and the method, after the human umbilical mesenchymal stem cell exosome and PDB-MSCs are cultured jointly, the PDB-MSCs is high in cell proliferation capability, good in cellular form and high in cellular activity, thus, the human umbilical mesenchymal stem cell exosome disclosed by the invention can be used for improving the quality of the PDB-MSCs, the cell factor VEGF and SCF secreting capability of the PDB-MSCs is improved effectively, thus, the problems of the PDB-MSCs that repeated transfer of generation is lowered in proliferation capability and poor in cell activity can be solved, and then, the large-scale culture and clinical application of the PDB-MSCs is facilitated effectively.

The invention belongs to the technical field of cell biology and particularly relates to culture and three-line differentiation induction methods of adipose tissue-derived mesenchymal stem cells. Theculture method specifically includes: placing adipose tissue into a centrifugal tube, adding normal saline, pipetting with a pipet, standing, sucking out liquid on the lower layer of the tissue, adding collagenase type I/PBS digestive juice with the concentration being 0.1% to perform digestion, and placing the culture bottle into a culture tank of 37 DEG C and with 5% CO2; taking out cells when the cells grow to 80% and merge, adding TrypLE digestive juice, terminating the digestion when the cells start retracting and rounding, and performing subculture and multiplication culture. The differentiation induction method has the advantages that differentiation time is shortened, the method needs 10 days to differentiate the cells into adipose and bones and needs 14 days to differentiate the cells into cartilage, and differentiation efficiency is increased greatly as compared a common induction method which needs 14 days to differentiate cells into adipose and needs 21 days to differentiate the cells into bones and cartilage.

The invention discloses a protoperidinuim culture medium and method. The protoperidinuim culture medium is composed of, by weight, 20-100 mg of NH4NO3, 20-60 mg of CO(NH2)2, 1-10 mg of KH2PO4, 100-800 mg of NaHCO3, 15-50 g of MnCl2 4H2O, 10-30 mg of FeC6H5O7 5H2O, 1*10-5-1*10-3 mg of VB12, 50-200 mg of VB1, 0.5-5 mg of KCl, 10-15 mg of Na2EDTA, 0.2-5 g of chitosan oligosaccharide with a molecular weight of 1500-2000 Da, and 1000 mL of seawater. The protoperidinuim culture method comprises the steps of phase one, conical flask culture and phase two, mineral water barrel culture. The protoperidinuim culture medium and method provides the optimal nutritive salt formula for protoperidinuim culture, thereby improving the yield of protoperidinuim poison and reducing the culture cost; compared with traditional outdoor open cement ponds expanded culture manners, culturing protoperidinuim in mineral water barrels is lower in cost, less prone to pollution and easier to operate and can greatly reduce workload, increase the growth speed of the protoperidinuim and shorten the culture period.

The invention belongs to the field of biology, and discloses a serum-free full-suspension domestication method for Sf9 cells. The method comprises the following steps: step 1, resuspending the Sf9 cells in an Sf-900 III SFM culture medium added with dextran sulfate, and diluting the Sf9 cells to 0.6-1.0x10<6> cells/mL; step 2, adding a heat-inactivated fetal calf serum to the product in the step 1, and culturing the Sf9 cells; and step 3, repeating the step 1 and the step 2 when the density of the Sf9 cells in the step 2 reaches 4x10<6> cells/mL or above in the third day, wherein the amount ofthe added heat-inactivated fetal calf serum is gradually reduced every time the step 1 and the step 2 are repeated until the density of the Sf9 cells still reaches 4x10<6> cells/mL or above without adding the heat-inactivated fetal calf serum, and the final concentration of dextran sulfate in the Sf-900 III SFM culture medium in the step 1 is 20-30 mg/L. The method has the advantages of low domestication frequency, high concentration of the domesticated cells after proliferation, and high survival rate.

The present invention provides means and methods for producing improved ex vivo B cell cultures with a short doubling time.

The invention belongs to the field of culture mediums, and particularly relates to a serum-free chemical component definition culture medium for EB66 cell strain suspension growth. The culture mediumcomprises an additive A including, according to the concentrations of the substances in the culture medium, 1-10 mg/L of insulin, 5-10 microgram/L of IGF-1, 0.5-2 mg of putrescine, 0.5-5 mg/L of glutathione, 1-5 mg/L of neovaricaine, 10-50 micromole of beta-mercaptoethanol, 1-5 mg/L of lecithin, 10-50 mg/L of vitamin C, 50-100 mg/L of taurine, 2-5 mg/L of cholesterol and 3-6 millimole of glutamine, wherein 1000-3000 mg of an amino acid mixed component is added to per liter of the culture medium. The culture medium supports rapid proliferation of EB66 cells under the serum-free and full-suspension conditions, and the doubling time is short. The cells are high in density. Besides, the invention further provides a preparation method of the culture medium.