51results about How to "Short doubling time" patented technology

The invention belongs to the field of environmental engineering, which relates to an integrated expanded granular sludge bed-membrane bioreactor whole-course autotrophy denitrification device and a process thereof. The device comprises a reactor main body, an air / liquid separation tank, a three-phase separation device, a membrane assembly, an air pump, an air flow meter, a perforated aeration pipe, a water feeding pump, a reflux pump, a water discharging pump and the like, wherein the reactor main body is divided into two function regions, i.e., a lower whole-course autotrophy denitrification function region and an upper membrane filtration function region, wherein an air aeration mode is adopted by the bottom of the immersion-type membrane assembly in the upper membrane filtration function region; the upper part of the reactor is externally connected with the air / liquid separation tank; the reflux system is used for refluxing heavy-oxygen-enriched water in the tank to a water inlet in the bottom of the reactor so as to provide the necessary dissolved oxygen for the whole-course autotrophy denitrification function region; and the fed sewage is firstly denitrified by the whole-course autotrophy denitrification function region and then filtered by the membrane so as to obtain the clean discharging water. The integrated reactor is small in land occupation. The whole-course autotrophy denitrification process is simple and convenient to operate.

A serum-free C3A clonal cell line and methods for generating the same are provided. The C3A cell line has a reduced doubling time in serum-free medium compared to a corresponding C3A cell line from which it is derived. Methods using the cells of the serum-free C3A clonal cell line for the production, expression and recovery of harvestable polypeptides, screening compounds for metabolic activity, studying enteric disease and for use in a bio-artificial liver device are also provided.

The present invention discloses a low-serum culture medium suitable for BHK-21 cell flask rotation culture, and a preparation method thereof. According to the present invention, in the flask rotation adherence culture, the serum consumption is substantially to 1% from the conventional 10%, the cell growth rate is rapid, the achieved cell density is high, and the high vitality is maintained; and during the culture medium development process, a plurality of compounds having special functions, lipid components, yeast, plant hydrolyzates and recombinant proteins are used to replace the conventionally-added animal-derived components in the culture medium, hormones, lipids, trace elements, hydrolyzates and other components are added to replace the partial functions of the serum so as to substantially reduce the serum addition amount, and reduce the serum cost while reduce the probability of the pollution caused by exogenous pathogenic bacteria carried by the serum and the animal-derived proteins in the culture medium, and the protein content in the culture medium is substantially reduced, such that the purification process at the late stage is easily achieved, and the stability of the cell product is improved.