Application of TROAP in preparation of product for detecting prognosis of breast cancer patient subjected to endocrinotherapy
A technology for endocrine therapy, breast cancer, applied in the field of biomedicine
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[0029] 1. Detect the viability of MCF-7 cells after letrozole treatment, and obtain the effective drug concentration.
[0030] The cells of each experimental group in the logarithmic growth phase were trypsinized, resuspended into a cell suspension, and counted. Cells were added to a 96-well plate, 100 μl per well, and the cell density of each group was observed under a microscope, and placed in 37°C, 5% CO 2 cultured in a cell culture incubator. On the second day, different concentrations of letrozole were added to each group, and the culture was continued. 10 μL of CCK-8 reagent was added to the wells 2-4 hours before the end of the culture, without changing the medium. After 4 hours, the 96-well plate was shaken on a shaker for 2-5 minutes, and the OD value was detected with a microplate reader at 450 nm. The effective drug concentration IC50 of letrozole was obtained by calculation for follow-up research.
[0031] 2. Screen differential genes.
[0032] MCF-7 cells adde...
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