Application of TROAP in preparation of product for detecting prognosis of breast cancer patient subjected to endocrinotherapy

A technology for endocrine therapy, breast cancer, applied in the field of biomedicine

Pending Publication Date: 2020-06-12
SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This gene has not been reported as a marker for detecting the effectiveness of letrozole treatment. We hope that in clinical practice, we will first detect this marker for patients with hormone receptor-positive breast cancer. Patients with positive expression will be treated with letrozole, with the purpose of improving Efficiency, slow down disease progression, prolong patient survival

Method used

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  • Application of TROAP in preparation of product for detecting prognosis of breast cancer patient subjected to endocrinotherapy
  • Application of TROAP in preparation of product for detecting prognosis of breast cancer patient subjected to endocrinotherapy
  • Application of TROAP in preparation of product for detecting prognosis of breast cancer patient subjected to endocrinotherapy

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Detect the viability of MCF-7 cells after letrozole treatment, and obtain the effective drug concentration.

[0030] The cells of each experimental group in the logarithmic growth phase were trypsinized, resuspended into a cell suspension, and counted. Cells were added to a 96-well plate, 100 μl per well, and the cell density of each group was observed under a microscope, and placed in 37°C, 5% CO 2 cultured in a cell culture incubator. On the second day, different concentrations of letrozole were added to each group, and the culture was continued. 10 μL of CCK-8 reagent was added to the wells 2-4 hours before the end of the culture, without changing the medium. After 4 hours, the 96-well plate was shaken on a shaker for 2-5 minutes, and the OD value was detected with a microplate reader at 450 nm. The effective drug concentration IC50 of letrozole was obtained by calculation for follow-up research.

[0031] 2. Screen differential genes.

[0032] MCF-7 cells adde...

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Abstract

The invention provides application of TROAP as a breast cancer endocrinotherapy drug screening molecular marker. It is found that the TROAP gene is related to the occurrence and development of breastcancer, the expression of the TROAP gene in a hormone receptor positive expression breast cancer patient is up-regulated, and after the expression of TROAP is down-regulated, the proliferation abilityof breast cancer MCF-7 cells is weakened. Based on this, the prognosis condition of a breast cancer patient subjected to endocrinotherapy is judged by detecting the expression level of TROAP.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the use of TROAP in preparing a product for detecting the prognosis of breast cancer patients undergoing endocrine therapy. Background technique [0002] Breast cancer is one of the malignant tumors that seriously threaten women's health. In recent years, its incidence has been increasing year by year all over the world, and it has leapt to the first place among female malignant tumors. The occurrence and development of breast cancer is affected by hormone levels in the body, and it is a hormone-dependent tumor. Endocrine therapy is mainly aimed at patients with positive hormone receptors. Because of its good tolerance and high safety, endocrine therapy has been widely used in clinic, and it can effectively prolong the disease-free survival and overall survival of breast cancer patients. Since 70%-80% of breast cancers are hormone-dependent tumors, endocrine therapy enjoys ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574
CPCC12Q1/6886G01N33/57415G01N33/57484C12Q2600/118C12Q2600/158C12Q2600/106G01N2333/47
Inventor 李凯赵力
Owner SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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