Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Adipose-derived stem cell-ECM modified SIS composite engineered bone and preparation method thereof

An adipose stem cell and human adipose stem cell technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as uncontrollable factors, and achieve the effect of good safety, high mechanical strength, and function to accelerate repair

Active Publication Date: 2017-07-21
河南源创生命干细胞库科技有限公司 +1
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, stem cell therapy has shown good tissue regeneration ability, but there are still great difficulties in improving the efficiency of cell therapy, mainly the following two problems: ① introduction of exogenous substances or the use of genetic engineering techniques to induce the differentiation of stem cells, There are many uncontrollable factors; ②When stem cells are injected into the body, how to quickly adhere and fuse into the recipient tissue to exert their functions is still under debate. Therefore, improving the efficiency of cell therapy is an issue that remains to be studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Adipose-derived stem cell-ECM modified SIS composite engineered bone and preparation method thereof
  • Adipose-derived stem cell-ECM modified SIS composite engineered bone and preparation method thereof
  • Adipose-derived stem cell-ECM modified SIS composite engineered bone and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation method of the fat stem cell-ECM modified SIS composite engineered bone of embodiment 1 comprises the following aseptic operation steps:

[0028] 1) Osteoblast culture and passage: Prepare α-MEM medium containing 10% fetal bovine serum as the osteoblast culture medium; place MC3T3-E1Subclone 14 cells isolated from the MC3T3-E1 cell line of the anterior cranial bone of the mouse into Cultivate in a cell culture incubator with 5% carbon dioxide concentration and 37°C, discard the old culture medium every 48 hours and add an equal amount of new culture medium, and carry out cell passage when the confluence of MC3T3-E1Subclone 14 cells is greater than 90% , the process of subculture of the cells is as follows: discard the old culture medium, add PBS buffer solution without calcium and magnesium ions, and wash twice; then add 1 mL of 0.25 wt% trypsin containing EDTA, and incubate at 37°C for 1 min; Collect the cells and add them to a centrifuge tube, centrifug...

Embodiment 2

[0032] The preparation method of the fat stem cell-ECM modified SIS composite engineered bone of embodiment 2 comprises the following aseptic operation steps:

[0033] 1) Osteoblast culture and passage: Prepare α-MEM medium containing 10% fetal bovine serum as the osteoblast culture medium; place MC3T3-E1Subclone 14 cells isolated from the MC3T3-E1 cell line of the anterior cranial bone of the mouse into Cultivate in a cell culture incubator with 5% carbon dioxide concentration and 37°C, discard the old culture medium every 48 hours and add an equal amount of new culture medium, and carry out cell passage when the confluence of MC3T3-E1Subclone 14 cells is greater than 90% , the process of subculture of the cells is as follows: discard the old culture medium, add PBS buffer without calcium and magnesium ions, wash 3 times; then add 1.5mL of 0.25wt% trypsin containing EDTA, and incubate at 37°C for 1min ;Collect the cells and add them to the centrifuge tube, and centrifuge at 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
elastic modulusaaaaaaaaaa
elastic modulusaaaaaaaaaa
elastic modulusaaaaaaaaaa
Login to View More

Abstract

The invention discloses an adipose-derived stem cell-ECM modified SIS composite engineered bone and a preparation method thereof. The microstructure of the composite engineered bone comprises an SIS collagen fiber skeleton, human adipose-derived stem cells and an extracellular matrix layer, the extracellular matrix layer is composed of MC3T3-E1 cell guided and secreted matrix protein and polysaccharide, the extracellular matrix layer covers the surface of the SIS collagen fiber skeleton, and human adipose-derived stem cells adhere to the extracellular matrix layer. The composite engineered bone has high mechanical strength and a microstructure highly similar to natural bone, can provide good tissue support at the initial stage of implantation in vivo, can provide excellent bone-formation microenvironment for implantation of stem cells, after implantation, human adipose-derived stem cells can undergo multiplication and osteogenic differentiation rapidly, thus promoting rapid repair of large bone injury. In addition, the composite engineered bone has pure natural and endogenous characteristics, can avoid uncontrollable factors brought about by introduction of exogenous substances / genes and the like, has very good safety, and has wide clinical application prospects in bone regeneration field.

Description

technical field [0001] The invention relates to the technical field of tissue engineering and stem cell therapy in biomedicine, in particular to an adipose stem cell-ECM modified SIS composite engineering bone applicable to bone regeneration and a preparation method thereof. Background technique [0002] About 3.5 million people in my country have bone defects due to different reasons every year, and about 1.5 million bone surgery transplants are performed. The demand for replacement bones is very large, especially for replacement bones for large bone injuries. Bone repair in vivo is a synergistic process between cells and scaffold materials. The three-dimensional microstructure of scaffold materials can provide host cells with a good microenvironment for adhesion, proliferation and migration. With the rapid development of tissue engineering technology, the use of tissue engineering principles to combine cells and scaffold materials in vitro and implant them into the body fo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/24A61L27/50
CPCA61L27/24A61L27/3633A61L27/365A61L27/3834A61L27/3847A61L27/3895A61L27/50A61L2430/02
Inventor 赵基源张迟李梅
Owner 河南源创生命干细胞库科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com