Food-borne selenium-rich antioxidative peptide and preparation method thereof

An antioxidant peptide, food-derived technology, applied in the field of food-derived selenium-rich antioxidant peptides and its preparation, can solve the problems of selenium-rich antioxidant peptide research and related patents that have not been reported, and selenium-rich antioxidant peptides are few , to achieve the effects of reducing subsequent desalination steps, reducing the generation of bitter peptides, and reducing costs

Inactive Publication Date: 2016-11-23
HENAN UNIVERSITY OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, among the published research results and patents, there are few studies on selenium-enriched antioxidant peptides, and they are mainly artificially synthesized selenium-enriched peptides, while research and related patents on food-derived and selenium-enriched antioxidant peptides have not yet been seen. to report

Method used

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  • Food-borne selenium-rich antioxidative peptide and preparation method thereof
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  • Food-borne selenium-rich antioxidative peptide and preparation method thereof

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preparation example Construction

[0032] The invention provides a preparation method of food-derived selenium-rich antioxidant peptides, such as figure 1 As shown, including the following steps:

[0033] 1) The selenium-rich rice bran is crushed and passed through a 60-100 mesh sieve, and immersed in a food-grade phosphate buffer with a material-to-liquid ratio (g / mL) of 14 to 18 and a pH of 8.0 to 9.5 at 20 to 45°C Extract for 2 to 4 hours, extract twice, centrifuge at 6000 revolutions / centrifuge for 20 minutes, combine the supernatant; adjust the pH of the supernatant to 4.5 with dilute hydrochloric acid, precipitate the protein, centrifuge, discard the supernatant, and wash the precipitated protein twice with water spare;

[0034] 2) In the precipitated protein obtained in step 1), add water with a mass / volume ratio of 16 to 1 12, stir and homogenize, and then mix the neutral protease to the precipitated protein with a mass ratio of 2% to 5% at a temperature of 45 Enzymatic hydrolysis at ~55℃ and pH 6.5-7.5 for...

Embodiment 1

[0037] The present invention provides a preparation method of food-derived selenium-enriched antioxidant peptide, which comprises the following steps:

[0038] 1) Selenium-enriched rice bran is crushed and passed through an 80-mesh sieve. In a food-grade phosphate buffer with a material-to-liquid ratio (g / mL) of 16 and a pH of 8.5, it is leached at 35°C for 3 hours, and leached twice. Centrifuge at 6000 rpm for 20 minutes and combine the supernatant; adjust the pH of the supernatant to 4.5 with dilute hydrochloric acid, precipitate the protein, centrifuge, discard the supernatant, and wash the precipitated protein twice for use;

[0039] 2) To the precipitated protein obtained in step 1), add water with a mass / volume ratio of 18, stir and homogenize, and then set the mass ratio of neutral protease to precipitated protein to 4%, temperature of 50°C, and pH of 7.0 Under the conditions of enzymatic hydrolysis for 1.2 hours, after enzymatic hydrolysis, adjust the enzymatic hydrolysis s...

Embodiment 2

[0042] The present invention provides a preparation method of food-derived selenium-enriched antioxidant peptide, which comprises the following steps:

[0043] 1) The selenium-rich rice bran is crushed and passed through a 60-mesh sieve, and extracted twice in a food-grade phosphate buffer with a material-to-liquid ratio (g / mL) of 18 and a pH of 8.0 at 45°C for 2 hours. Centrifuge at 6000 rpm for 20 minutes and combine the supernatant; adjust the pH of the supernatant to 4.5 with dilute hydrochloric acid, precipitate the protein, centrifuge, discard the supernatant, and wash the precipitated protein twice for use;

[0044] 2) In the precipitated protein obtained in step 1), add water with a mass / volume ratio of 16 and stir and homogenize. Then, the mass ratio of neutral protease to precipitated protein is 5%, the temperature is 45°C, and the pH value is 7.5. Enzymatic hydrolysis under conditions for 1.0 hour, after enzymatic hydrolysis, adjust the enzymatic hydrolysis solution to n...

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Abstract

The invention discloses a food-borne selenium-rich antioxidative peptide and a preparation method thereof. A by-product, namely selenium-rich rice bran obtained through selenium-rich brown rice processing serves as a raw material and is preprocessed, then, selenoprotein is extracted, the selenoprotein is subjected to composite enzymatic hydrolysis, separation and purification and activity checking, and then the selenium-rich peptide with antioxidant activity is obtained. The method has the advantages that safety is high, conditions are mild, a process is simple, and product activity is high. When applied to foods and especially to functional foods, the obtained selenium-rich antioxidative peptide can play a role of antioxidation of an active peptide, can also improve the intake of selenium in food of people and has multiple beneficial effects. In addition, the selenium-rich rice bran is the by-product produced through selenium-rich brown rice processing, and the selenium-rich antioxidative peptide prepared from the selenium-rich rice bran can effectively improve the resource utilization rate and increase the added value of the product.

Description

Technical field [0001] The invention belongs to the technical field of agricultural product processing, and specifically relates to a food-derived selenium-rich antioxidant peptide and a preparation method thereof. Background technique [0002] The existence of life depends on oxygen, and oxidative metabolism in the body is necessary for cell survival. At the same time, oxidative metabolism in the body produces a large number of free radicals and other reactive oxygen species. Their excessive accumulation in the body can damage biological macromolecules such as DNA, protein, and fatty acids, leading to oxidative damage to their structure and function, and causing cancer, A series of diseases such as cardiovascular disease. Eliminating excess radicals and other reactive oxygen species in the body is of great significance for preventing the occurrence of diseases and maintaining the health of the body. Synthetic antioxidants such as butyl hydroxyanisole (BHA), 2,6-di-tert-butyl p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/12
CPCA23J1/125A23V2002/00A23V2200/30
Inventor 刘昆仑陈复生赵妍方勇胡秋辉布冠好张丽芬辛颖赵爽
Owner HENAN UNIVERSITY OF TECHNOLOGY
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