Production method of yoghourt having functions of assisting in reducing blood pressure and protecting liver
A production method and blood pressure lowering technology, applied in milk preparations, dairy products, applications, etc., can solve side effects and other problems, achieve the effect of relaxing blood vessels, maintaining blood pressure stability, lowering blood pressure and protecting liver function
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Embodiment 1
[0033](1) Add pepsin to the aqueous solution of casein with substrate concentration of 7% according to the ratio of enzyme to substrate of 2000U / g, the hydrolysis conditions of pepsin are: temperature is 37℃, pH3.0, hydrolysis After 3h, heat in a boiling water bath for 10min to end the reaction, and cool to 45-50°C. Then the pH value of the hydrolyzate was adjusted to 7.7, and the hydrolyzate was continued with trypsin. The ratio of trypsin to substrate was 500U / g. After 3h of hydrolysis, the hydrolyzate was boiled in a boiling water bath for 10min to inactivate the enzyme, and then cooled to 45-50°C. Adjust the pH value of the hydrolyzate to 7.0, add flavor protease for debittering, the ratio of flavor protease to substrate is 280U / g, and the hydrolysis time is 2 hours. The hydrolyzed solution is boiled in a water bath to inactivate the enzyme for 10 minutes, then ultrafiltered with a 10-15kD ultrafiltration membrane, and then desalted with a nanofiltration membrane with a mo...
Embodiment 2
[0041] In the raw material formula for producing 1000g of yogurt, 3g of casein hydrolyzate rich in ACE inhibitory peptide and 1.5g of purple potato extract rich in anthocyanin were added. After mixing evenly, it was sterilized at 95°C for 5 minutes, and fermented at 43°C for 5.5 hours after inoculation. After ripening at 4°C for 12 hours, the blood pressure-lowering and liver-protecting functions were tested.
[0042] (1) Blood pressure lowering function test
[0043] 16 12-week-old male SHRs, with a body weight of (260±15) g, had free access to food and water, kept at an ambient temperature of (23±1)°C, and a relative humidity of (60±5)%, pre-feeding for 1 week (Tianjin Medical University animal laboratory). Rats were randomly divided into 2 groups according to body weight, 8 rats in each group, which were used as blank group and sample group respectively. at 3.3g / kg m b Dose of SHR was administered orally, and the blank group was administered with yogurt (3.3g / kg m b );...
Embodiment 3
[0054] In the raw material formula for producing 1000g yogurt, 3g of casein hydrolyzate rich in ACE inhibitory peptide and 3g of purple potato extract rich in anthocyanin are added. After mixing evenly, it was sterilized at 95°C for 5 minutes, and fermented at 43°C for 5.5 hours after inoculation. After ripening at 4°C for 12 hours, the blood pressure-lowering and liver-protecting functions were tested.
[0055] (1) blood pressure lowering function detection, method is the same as (1) in embodiment 1, see the result Figure 8 . Depend on Figure 8 It can be seen that yogurt (3g casein hydrolyzate + 3g purple potato extract / 1000g yogurt) added with ACE-inhibiting peptide-rich casein hydrolyzate and anthocyanin-rich purple potato extract can increase the blood pressure of SHR 4h after gavage. Compared with ordinary yogurt (13mmHg), there is a significant difference (P<0.05).
[0056] (2) Evaluation of liver protection function, the method is the same as (2) in Example 1, the...
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