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A kind of L-citrulline succinate and its preparation method and application

A technology of citrulline succinate and citrulline, which is applied in the field of medicine and chemical industry, can solve the problems of lack of L-citrulline succinate, etc., to enhance the body's immunity, treat sexual dysfunction, and the preparation method is simple and feasible Effect

Active Publication Date: 2018-12-14
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on L-citrulline succinate in China

Method used

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  • A kind of L-citrulline succinate and its preparation method and application
  • A kind of L-citrulline succinate and its preparation method and application
  • A kind of L-citrulline succinate and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Microorganism fermentation method prepares L-citrulline aqueous solution, comprises the steps:

[0039] (1) plate activation, inoculate the bacterial strain (preservation number is CGMCC No.11567) on LB medium under aseptic conditions for cultivation, the cultivation temperature is 30°C, and the cultivation time is 24h;

[0040] (2) Expanded cultivation: pick a single colony from the LB plate of step (1) and receive it in the seed medium, the temperature of the expanded cultivation is 30°C, the shaking table speed is 200rpm, and the incubation time is 12h;

[0041] Seed medium (g / L): glucose 20, dipotassium hydrogen phosphate trihydrate 1.5, potassium dihydrogen phosphate 0.5, urea 2.5, manganese sulfate monohydrate 0.02, ferrous sulfate heptahydrate 0.02, biotin 10 -4 , VB1 2×10 -4 , arginine 0.2, magnesium sulfate heptahydrate 0.4, potassium hydroxide to adjust the pH to 6.5;

[0042] (3) Fermentation culture in a 5L fermenter: inoculate the expanded cultured bacter...

Embodiment 2

[0046] Configure the L-citrulline prepared in Example 1 into an aqueous solution with a concentration of 223g / L, take 20ml, add 1.5g of succinic acid, stir the reaction solution at 50°C until it becomes transparent (stirring time 40-45min), and dissolve the solution Cool down to room temperature, and add 11ml of absolute ethanol to make the solution quickly approach saturation. At room temperature, add 90 ml of 80% ethanol at 0.48 ml / min and keep stirring, the stirring speed is 200 rpm. Slowly lower the temperature of the solution to 4°C and cool overnight to crystallize, filter, wash, and dry the crystals in a vacuum oven. The product is 3.53g, and the yield is 64.4%. The infrared spectrum of the product is shown in figure 1 As shown in the hydrogen spectrum, see figure 2 shown in the liquid chromatogram image 3 As shown, the purity measured by high performance liquid chromatography was 97.5%.

Embodiment 3

[0048] Purify the L-citrulline fermented liquid prepared in Example 1 to obtain an aqueous solution of L-citrulline, concentrate the aqueous solution to 200g / L, take 22ml, add 1.5g of succinic acid, 0.5g of palladium Carbon, the temperature is 55°C and the reaction solution is stirred until it is transparent (stirring time is about 20-25min), the solution is cooled to room temperature, and 11ml of absolute ethanol is added to make the solution quickly approach a saturated state. At room temperature, 90 ml of 80% ethanol was added at 0.5 ml / min and stirred continuously, and the stirring speed was 150 rpm. Slowly lower the temperature of the solution to 4°C and cool overnight to crystallize, filter, wash, and dry the crystals in a vacuum oven. The product is 3.6406 g, the yield is 66.35%, and the purity measured by high performance liquid chromatography is 98.1%. After adding the catalyst, the reaction rate is nearly doubled, that is, the reaction time is shortened from 40-45min...

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Abstract

The invention discloses L-citrulline succinate and a preparation method and application thereof. The method comprises the steps of firstly preparing an L-citrulline water solution, adding succinic acid powder to the solution, and carrying out stirring reaction to obtain an L-citrulline succinate solution; and separating out the L-citrulline succinate solution in a crystal form. The method is simple, feasible, low in production cost, high in product yield and high in purity; and industrial popularization can be carried out. Through the L-citrulline succinate, blood vessels of a person can be relaxed; and the L-citrulline succinate is used for strengthening the male sexual function, keeping cholesterol at a normal level and synthesizing an antidote and a sedative.

Description

technical field [0001] The invention belongs to the field of medicine and chemical industry, and discloses an L-citrulline succinate and its preparation method and application. technical background [0002] In China, the research on citrulline is still in the stage of understanding, there is no corresponding report on the production method, and there are not many researches on its detection method and physiological function, only a small number of literature reports. In terms of the physiological functions of citrulline, foreign scientists have found that citrulline has some very important pharmacological functions, such as free radical scavenging, which is of great significance in the health care of the human body; vasodilation, which is expected Become a good medicine for the treatment of endotoxicity and sepsis. A Japanese scientist has developed a new peptide of citrulline and developed the new peptide into an anti-AIDS drug. At the same time, citrulline is considered ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C273/18C07C275/16A61K31/198A61P37/02A61P9/00A61P15/10A61P25/20A61P39/02A61P15/00
CPCC07C273/1854C07C275/16
Inventor 郝宁丁叶胡洁刘兆星许琳欧阳平凯
Owner NANJING TECH UNIV
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