Stropharia rugoso-annulata strain breeding method

Abstract

The invention discloses a stropharia rugoso-annulata strain breeding method. The breeding method comprises the following steps of preparing a stropharia rugoso-annulata rejuvenation culture medium, inoculating stropharia rugoso-annulata mother strains to the rejuvenation culture medium for variable-temperature rejuvenation pure culture, and cultivating stress-resistant and high-activity strophariarugoso-annulata mother strains; preparing a shake-flask liquid culture medium, inoculating the rejuvenated stropharia rugoso-annulata mother strains onto the shake-flask liquid culture medium, and cultivating the stropharia rugoso-annulata shake-flask liquid strain by adopting a shake-flask liquid submerged fermentation process; preparing a fermentation tank fermentation culture medium, inoculating the cultured shake-flask liquid strain onto the fermentation tank fermentation culture medium, and cultivating a liquid submerged fermentation strain of stropharia rugoso-annulata by adopting a fermentation tank liquid submerged fermentation process. A composting fermentation method is adopted, the stropharia rugoso-annulata cultivar culture medium is prepared, the prepared cultivar fermentation culture medium is put into a cultivar breeding tray hole, then a cultivated stropharia rugoso-annulata liquid submerged fermentation strain is inoculated to the cultivar fermentation culture mediumin the breeding tray hole through an inoculation gun, and the stropharia rugoso-annulata cultivar is obtained through an open solid fermentation process.

Description

technical field [0001] The invention belongs to the field of breeding of edible fungus strains, and in particular relates to a breeding method of Stropharia stropharia. Background technique [0002] Stropharia belongs to Basidiomycota, Agaricaceae, Agaricaceae, Strophariaceae, and Stropharia. Stropharia is not only rich in nutrients such as dietary fiber, mineral elements, and vitamins, but also rich in active ingredients such as protein and polysaccharides, which can regulate human immunity, resist oxidation, inhibit tumor cells, and lower blood sugar. It is one of the top ten mushrooms in the international mushroom trading market, and it is also an important edible fungi variety recommended by the United Nations to developing countries for cultivation. [0003] In the 1960s, Germany began to try to grow Stropharia, and then Poland, Hungary and other countries successively introduced and cultivated it. my country began to introduce and try to grow it in the 1980s, and it w...

AI Technical Summary

Problems solved by technology

In recent years, while the cultivation of Stropharia stropharia in my country has been successfully cultivated and continuously promoted, there have been many problems in the breeding of Stropharia stropharia cultivars, such as high pollution rate, easy aging of strains, long breeding cycle and high production cost. The high cost of stropharia cultivation, unstable yield and quality restrict the sustainable development of the stropharia industry

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