Fast detecting method for benzopyrene in edible oil

A detection method and edible oil technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as lack of confidence of consumers in camellia oil products, unfavorable health and rapid development of camellia oil industry, and threats to the safety of experimenters. Low cost, easy operation, and short elution time

Inactive Publication Date: 2017-03-15
WUXI X RES PROD DESIGN & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since September 2010, the products of many domestic camellia oil production enterprises have been detected to exceed the standard of carcinogen benzopyrene, which caused extreme shocks in the industry, caused consumers to lack confidence in camellia oil products, and severely hit the camellia oil market , is not conducive to the healthy and rapid development of the camellia oil industry
However, in the current benzopyrene detection method, the sample pretreatment method is complicated and the steps are cumbersome, and it is easy to inhale or skin contact with benzopyrene due to careless operation during the pretreatment sample process, which brings safety hazards to the experimenters. great threat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In an embodiment of the present invention, a rapid detection method for benzopyrene in edible oil comprises the following steps:

[0024] (1) Preparation of the test solution: Accurately weigh an appropriate amount of edible oil sample into a stoppered conical flask, add petroleum ether equivalent to 16 times the amount of the edible oil sample, and shake after plugging; After the bottle mouth of the shaped bottle is sealed, ultrasonic extraction is carried out. The ultrasonic frequency is 35kHz, the ultrasonic power is 400W, the time is 22min, and the supernatant is collected by centrifugation at 12000r / min for 10min; 12 times the amount of petroleum ether, after sealing the mouth of the Erlenmeyer flask with a stopper with a sealing film, ultrasonic extraction, the ultrasonic frequency is 35kHz, the ultrasonic power is 400W, the time is 18min, centrifuged at 12000r / min for 10min, and the supernatant is combined Get sherwood oil and add the solid-phase extraction colum...

Embodiment 2

[0028] In an embodiment of the present invention, a rapid detection method for benzopyrene in edible oil comprises the following steps:

[0029] (1) Preparation of the test solution: Accurately weigh an appropriate amount of edible oil sample into a stoppered Erlenmeyer flask, add xylene equivalent to 18 times the amount of the edible oil sample, and shake after plugging; After the bottle mouth of the shaped bottle is sealed, ultrasonic extraction is carried out. The ultrasonic frequency is 40kHz, the ultrasonic power is 450W, the time is 15min, and the supernatant is collected by centrifugation at 18000r / min for 5min; 8 times the amount of xylene, after sealing the mouth of the Erlenmeyer flask with stopper with a sealing film, ultrasonic extraction, the ultrasonic frequency is 40kHz, the ultrasonic power is 450W, the time is 12min, centrifuged at 18000r / min for 5min, and the supernatant is combined Get xylene and add solid-phase extraction column to activate, until xylene dr...

Embodiment 3

[0033] In an embodiment of the present invention, a rapid detection method for benzopyrene in edible oil comprises the following steps:

[0034] (1) Preparation of the test solution: Accurately weigh an appropriate amount of edible oil sample into a stoppered Erlenmeyer flask, add 17 times the amount of chloroform equivalent to the edible oil sample, shake after plugging; After the bottle mouth of the bottle is sealed, ultrasonic extraction is carried out. The ultrasonic frequency is 40kHz, the ultrasonic power is 400W, the time is 20min, and the supernatant is collected by centrifugation at 15000r / min for 8min; the residual edible oil sample is then added to the residual edible oil sample for 10 minutes. Double the amount of chloroform, seal the mouth of the Erlenmeyer flask with a stopper with a sealing film, and then extract it ultrasonically. The ultrasonic frequency is 35kHz, the ultrasonic power is 450W, the time is 15min, centrifuged at 15000r / min for 8min, and the super...

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PUM

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Abstract

The invention discloses a fast detecting method for benzopyrene in edible oil. The method comprises the steps of 1, preparation of a solution for a testing product, wherein a proper amount of edible oil sample is accurately weighed and placed in a conical flask with a cover, an organic solvent is added, vibrating and shaking are carried outer after covering, ultrasonic extraction is carried out, supernate is collected after centrifugation, an organic solvent is added into the residual edible oil sample, ultrasonic extraction and centrifugation are carried out, the supernate is mixed, an organic solvent is taken and added into a solid phase extraction column for activation, the mixed supernate is transferred into the activated solid phase extraction column after activation is completed, the organic solvent is used for elution, eluant is collected into a measuring flask, volume metering is carried out, and the eluant passes through a 0.22-micrometer microfiltration membrane and enters a sample bottle for use; 2, preparation of a standard product solution; 3, detection, wherein a benzopyrene standard series solution and the solution for the testing product are sequentially injected into HPLC for detection. Two times of ultrasonic extraction and solid phase extraction column enrichment and purification are adopted, and the method is easy, convenient and safe to operate, low in cost, complete in extraction and accurate, true and reliable in detecting result.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a rapid detection method for benzopyrene in edible oil. Background technique [0002] Benzopyrene, also known as benzo(a)pyrene, abbreviated as BaP, is a class of organic compounds with obvious carcinogenic effects. It is a polycyclic aromatic hydrocarbon compound composed of a benzene ring and a pyrene molecule. It has been tested that B(a)P will be produced in high-temperature vegetable oils and over-fried foods that have been used many times. When high-temperature frying is used repeatedly, the organic matter in the food will be decomposed by heat, and B(a)P will be formed through cyclization and polymerization. Carcinogenic ingredients. [0003] Incidents of benzopyrene exceeding the standard often occur in vegetable oils. In 1996, 40 olive oil samples on the Brazilian market were found to contain benzopyrene, with the highest content reaching 164 μg / kg. In July 200...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 徐静赵春城胡勇蒋韦艳刘金杰吴敏芳
Owner WUXI X RES PROD DESIGN & RES
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