40results about How to "Complete elution" patented technology

The invention discloses an extraction and separation method for artemisinin. The extraction and separation method comprises the following steps: (1) grinding abrotani herba serving as a raw medicinal material after the artemisinin is dried; (2) performing backflow extraction on the material by petroleum ether or solvent gasoline or a mixture of the petroleum ether and the solvent gasoline, which serves as a solvent, twice for 1-10 hours according to that the volume-mass ratio of the solvent to the material is (2:1)-(15:1), intermittently vacuumizing an extraction pot and breaking vacuum in an extraction process, and combining and concentrating extracting solutions of the two times for later use; (3) performing silica gel column chromatography on the concentrated artemisinin extracting solution, then eluting the artemisinin extracting solution through a normal hexane and diethyl ether mixture, and concentrating, decoloring, drying and crystallizing an eluant to obtain the artemisinin. According to the extraction separation method for the artemisinin, vacuum pulse type extraction and ultrasonic auxiliary elution are organically combined, so that low cost and high efficiency for extracting the artemisinin from the abrotani herba are achieved, and the application prospect is wide.

The invention discloses a carbon nano tube surface lysozyme molecularly-imprinted polymer. The carbon nano tube surface lysozyme molecularly-imprinted polymer adopts lysozyme as a template molecule, adopts 1-allyl-3-methylimidazolium hexafluorophate ionic liquid and N-tert-butyl acrylamide as a mixing functional monomer, adopts an acrylamide modified carbon nanotube as a matrix, adopts a Tris-HCl buffer solution with the pH being 9 as a reaction system, is obtained under the effect of a cross-linking agent, an initiating agent and a catalyst and has the selective recognition capacity for lysozyme. The number of acting sites is multiple when the template molecule and the functional monomer are combined, the acting force is higher, more effective recognition sites can be formed in the preparation process of the molecularly-imprinted polymer, and therefore the printing effect and the preferential adsorption capacity for lysozyme are better.

The invention discloses a fast detecting method for benzopyrene in edible oil. The method comprises the steps of 1, preparation of a solution for a testing product, wherein a proper amount of edible oil sample is accurately weighed and placed in a conical flask with a cover, an organic solvent is added, vibrating and shaking are carried outer after covering, ultrasonic extraction is carried out, supernate is collected after centrifugation, an organic solvent is added into the residual edible oil sample, ultrasonic extraction and centrifugation are carried out, the supernate is mixed, an organic solvent is taken and added into a solid phase extraction column for activation, the mixed supernate is transferred into the activated solid phase extraction column after activation is completed, the organic solvent is used for elution, eluant is collected into a measuring flask, volume metering is carried out, and the eluant passes through a 0.22-micrometer microfiltration membrane and enters a sample bottle for use; 2, preparation of a standard product solution; 3, detection, wherein a benzopyrene standard series solution and the solution for the testing product are sequentially injected into HPLC for detection. Two times of ultrasonic extraction and solid phase extraction column enrichment and purification are adopted, and the method is easy, convenient and safe to operate, low in cost, complete in extraction and accurate, true and reliable in detecting result.

The invention discloses a preparation method of a high-adsorbability ion exchange resin for sewage treatment. The preparation method comprises the following steps: sending macroporous chloromethylatedpolystyrene resin into a piperazine aqueous solution for swelling, adding 2-aminoethanol, carrying out heating, stirring, cooling and washing, adding modified guar gum, stirring the mixture uniformly, and carrying out filtering, washing and drying to obtain pretreated polystyrene resin; and dropwise adding thionyl chloride into the pretreated polystyrene resin in an ice water bath, adjusting thetemperature, keeping the temperature, carrying out cooling, washing and filtering, sending the mixture into nitrobenzene for swelling, and adding trimethylamine for reaction to obtain the high-adsorbability ion exchange resin with for sewage treatment. The modified guar gum is prepared by the following processes: mixing chitosan and glacial acetic acid uniformly, adding water, stirring the materials uniformly, adding polyethylene glycol, carrying out heating and stirring, then carrying out heating, adding guar gum and bamboo charcoal powder and stirring the materials to obtain the modified guar gum.

The invention provides a preparation method of an estrogen specific nanometer silica gel surface molecule marking material, mainly comprising three steps of surface modification of a colloidal silica sol nanosphere, polyreaction and template molecule elution. The preparation method is easy to control, required experimental facilities are simple, and a prepared nanometer colloidal silica gel molecule marking polymer has the advantages of big specific surface, high molecule binding capacity, many recognition sites, favorable molecular recognition performance and the like. An estrogen molecule marking polymer material prepared by the invention not only can be used for various ideal adsorbents or coating materials of a solid-phase extraction technology, but also can be used for preparing molecule marking sensors and chips. The invention adopting the technology is of great importance in analyzing and researching various environmental pollutant residues.

The invention discloses a detection method of counterfeiting honey in a traditional Chinese medicine honey pill. A high performance liquid chromatography method is used for analysis. The method comprises the following detection steps of (1) preparing a mixed reference substance solution; (2) preparing a sample solution; (3) performing chromatography analysis; (4) obtaining the result: in the testsubstance chromatography, the result of chromatography peak occurrence in the corresponding position after the maltopentaose of a mixed reference substance chromatography shows the oligosaccharide inspection, and the result indicates that the honey is counterfeiting. The detection method provided by the invention has the advantages that the high performance liquid chromatography method is used foranalysis; the operation is simple and convenient; convenience and high speed are realized; the detection efficiency is high; the sample solution preparation method is simple; the separation effect isgood; the accuracy is high; good stability and reproducibility are realized; the counterfeiting honey in various kinds of traditional Chinese medicine honey pills can be detected at the same time; the use range is wide.

The invention discloses a method for improving purity of a bispecific antibody by purification with magnetic beads. The method comprises the steps of mixing magnetic beads coated with activated Protein A with a bispecific antibody containing supernatant to be purified, performing incubation, and after the magnetic beads are sufficiently mixed with the bispecific antibody, removing the supernatant through magnetic separation; firstly, cleaning the magnetic beads with a balanced buffered solution for 2-5 times; cleaning the magnetic beads with water; eluting the cleaned magnetic beads with an eluent, performing magnetic separation, and collecting the eluent; and eluting the separated magnetic beads with the eluent for 1-3 times, and merging the eluent so as to obtain the purified bispecific antibody. The method does not need the clarification operation, so that the time saved, the demand for equipment is reduced, and the bispecific antibody of samples being higher in flux and larger in volume can be purified within a short time.

The invention discloses a multifunctional bottom mud eluting-disturbing device, namely a multifunctional eluting-disturbing head, which is arranged at the front end of a bottom mud eluting ship and has multiple disturbing modes. The device comprises a jet device, an aeration device and an electric mechanical disturbing device, which are independently arranged in an elution box and are connected with a control box. The aeration device is arranged in the elution box and is used for carrying out aeration on riverway bottom mud and filling oxygen into riverway bottom mud, releasing pollutants on the surface layer of the bottom mud into an elution chamber, and pumping the bottom mud to the shore. The jet device ejects high pressure water flow, which is formed by making water pumped by a high pressure pump go through a jet hole, on riverway bottom mud so as to disperse the pollutant layer on the bottom mud, the pollutants are released and delivered to the shore. The mechanical disturbance isthat the river bottom mud is disturbed, rotated and scattered through the rotation of a disturbing head, so that pollutants are released. The adaptability of the bottom mud eluting ship to differentkinds of riverway bottom mud is improved, the bottom mud eluting ship can better adapt to the field environment, and the working efficiency and the treatment capacity of the bottom mud eluting ship are improved.

The invention relates the oral mucosa agent. The invention improves the effects of collection and elution. The one end of hand handle 1 of oral mucosa agent has striking board 2, and the two sides of striking board have ridges 3. Between ridges 3 there are via holes(2-1).

The invention discloses a gas washing device. The gas washing device comprises a first tower, a second tower, a connection pipe and an acid solution tank. A first filler part is installed in the first tower, the top of the first tower is provided with a first opening, and a first steam pipe is elastically connected to the portion, corresponding to the first opening, of the first tower and provided with a steam inlet; a second filler part is at least installed in the second tower, the top of the second tower is provided with a second opening, and a second steam pipe is elastically connected to the portion, corresponding to the second opening, of the second tower and provided with a steam outlet; the connection pipe is elastically connected to the bottom of the first tower and the bottom of the second tower separately; the acid solution tank is communicated with the connection pipe, communicated with the first tower through a first pipe via the first steam pipe and communicated with a second tower through the second pipe via the second steam pipe. According to the gas washing device, ammonia nitrogen in steam can be completely absorbed, the treatment efficiency is improved, leakage is avoided, and the cost is low.