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Lysozyme molecularly imprinted polymer on carbon nanotube surface and preparation method thereof

A carbon nanotube and molecular imprinting technology, applied in the field of chemistry and materials, can solve the problems of large protein volume, many binding sites, and unstable conformation, and achieve the effects of large specific surface area, complete elution and rapid adsorption.

Inactive Publication Date: 2016-06-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the imprinting of biological macromolecules such as proteins still faces great challenges due to the problems of large size, complex structure, many binding sites, and unstable conformation of the protein itself as an imprinting template.

Method used

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  • Lysozyme molecularly imprinted polymer on carbon nanotube surface and preparation method thereof
  • Lysozyme molecularly imprinted polymer on carbon nanotube surface and preparation method thereof
  • Lysozyme molecularly imprinted polymer on carbon nanotube surface and preparation method thereof

Examples

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Effect test

Embodiment 1

[0049] Dissolve 18 mg of lysozyme in 30 mL of Tris-HCl buffer solution at pH 9, add 200 mg of 1-allyl-3-methylimidazolium hexafluorophosphate ionic liquid, stir magnetically at room temperature for 2 hours; add 30 mg of acrylamide-modified carbon nanotubes, and disperse them by ultrasonic Uniformly, add 100mg N,N'-methylenebisacrylamide, nitrogen deoxygenation for 15 minutes; add 40mg ammonium persulfate, 50μL N,N,N',N'-tetramethylethylenediamine, continue nitrogen deoxygenation 15 minutes, and then magnetically stirred at room temperature for 48 hours under anaerobic conditions. The solid-liquid two phases were separated, and the solid-phase product was washed 5 times with a Tris-HCl buffer solution of pH 9, then eluted with ultrapure water until no protein remained, and freeze-dried to obtain a molecularly imprinted polymer of lysozyme on the surface of carbon nanotubes. The synthesis and elution methods of the blank molecularly imprinted polymer are the same, except that th...

Embodiment 2

[0051] Dissolve 18 mg of lysozyme in 30 mL of Tris-HCl buffer solution at pH 9, add 300 mg of 1-allyl-3-methylimidazolium hexafluorophosphate ionic liquid, stir magnetically for 2 hours at room temperature; add 30 mg of acrylamide-modified carbon nanotubes, and disperse them by ultrasonic Uniformly, add 100mg N,N'-methylenebisacrylamide, nitrogen deoxygenation for 15 minutes; add 40mg ammonium persulfate, 50μL N,N,N',N'-tetramethylethylenediamine, continue nitrogen deoxygenation 15 minutes, and then magnetically stirred at room temperature for 48 hours under anaerobic conditions. The solid-liquid two phases were separated, and the solid-phase product was washed 5 times with a Tris-HCl buffer solution of pH 9, then eluted with ultrapure water until no protein remained, and freeze-dried to obtain a molecularly imprinted polymer of lysozyme on the surface of carbon nanotubes. The synthesis and elution methods of the blank molecularly imprinted polymer are the same, except that th...

Embodiment 3

[0053] Dissolve 18 mg of lysozyme in 30 mL of Tris-HCl buffer solution at pH 9, add 350 mg of 1-allyl-3-methylimidazolium hexafluorophosphate ionic liquid, stir magnetically for 2 hours at room temperature; add 30 mg of acrylamide-modified carbon nanotubes, and disperse them by ultrasonic Uniformly, add 100mg N,N'-methylenebisacrylamide, nitrogen deoxygenation for 15 minutes; add 40mg ammonium persulfate, 50μL N,N,N',N'-tetramethylethylenediamine, continue nitrogen deoxygenation 15 minutes, and then magnetically stirred at room temperature for 48 hours under anaerobic conditions. The solid-liquid two phases were separated, and the solid-phase product was washed 5 times with a Tris-HCl buffer solution of pH 9, then eluted with ultrapure water until no protein remained, and freeze-dried to obtain a molecularly imprinted polymer of lysozyme on the surface of carbon nanotubes. The synthesis and elution methods of the blank molecularly imprinted polymer are the same, except that th...

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Abstract

The invention discloses a carbon nano tube surface lysozyme molecularly-imprinted polymer. The carbon nano tube surface lysozyme molecularly-imprinted polymer adopts lysozyme as a template molecule, adopts 1-allyl-3-methylimidazolium hexafluorophate ionic liquid and N-tert-butyl acrylamide as a mixing functional monomer, adopts an acrylamide modified carbon nanotube as a matrix, adopts a Tris-HCl buffer solution with the pH being 9 as a reaction system, is obtained under the effect of a cross-linking agent, an initiating agent and a catalyst and has the selective recognition capacity for lysozyme. The number of acting sites is multiple when the template molecule and the functional monomer are combined, the acting force is higher, more effective recognition sites can be formed in the preparation process of the molecularly-imprinted polymer, and therefore the printing effect and the preferential adsorption capacity for lysozyme are better.

Description

technical field [0001] The invention belongs to the field of chemistry and materials, and relates to a molecularly imprinted polymer of lysozyme on the surface of carbon nanotubes and a preparation method thereof. Background technique [0002] Molecular imprinting technology is a technology that simulates antigen-antibody interaction and obtains a polymer whose spatial structure and binding site match the template molecule by artificial synthesis. First, complementary interactions between template molecules and functional monomers form complexes, and then under the action of cross-linking agents, bulk polymerization occurs around the template-monomer complexes to form polymers, and finally the polymerization is removed under certain conditions. The template molecules in the material can be used to obtain selective molecular recognition materials with specific spatial structure holes and recognition sites matching the template molecules, which are commonly referred to as mole...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F292/00C08F222/38C08F220/54C08J9/28C09C1/44C09C3/08
Inventor 刘名茗王小洁皮江燕黄蓉杜亚媚冯雄汉刘凡
Owner HUAZHONG AGRI UNIV
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