Alcohol-free nucleic acid detection reagent tube

Abstract

The invention discloses an alcohol-free nucleic acid detection reagent tube, which comprises a main pipe (1), and a plurality of branch pipes (2) are arranged at the lower end of the main pipe (1); the main pipe (1) is sequentially provided with cracking zones ( 3), the first separation plug (4), the cleaning area (5), the second separation plug (6), the branch pipe (2) is provided with the reaction zone (7), and the second separation plug (6) is located in the branch pipe (2) ) and the junction of the main pipe (1), the first separation plug (4) and the second separation plug (6) are provided with a liquid or solid hydrophobic layer (8); the inner wall of the main pipe (1) is provided with There is a magnetic bead channel (9) through to the branch pipe (2); a nucleic acid extraction solution is provided in the lysis zone (3), an alcohol-free cleaning solution is provided in the cleaning zone (5), and an alcohol-free cleaning solution is provided in the reaction zone (7). eluent. The invention can not only reduce the difficulty of operation, but also improve the accuracy and efficiency of detection.

Description

technical field [0001] The invention relates to a nucleic acid detection tube, in particular to an alcohol-free nucleic acid detection reagent tube. Background technique [0002] The nucleic acid detection cycle in the laboratory is short, and the sensitivity and specificity are comparable to those of the culture method. At present, nucleic acid detection reagent tubes are usually used for nucleic acid detection of samples. Separate, and then melt the separation layer by heating, so that the magnetic beads can carry nucleic acid through the separation layer for corresponding operations, but because nucleic acid is sensitive to temperature changes, in order to reduce the influence of temperature changes on nucleic acids, operators need to strictly control the heating area, so It will increase the difficulty of operation. At the same time, because the existing detection tubes are susceptible to the interference of many factors (such as the interference of temperature on nucle...

AI Technical Summary

Problems solved by technology

[0002] The nucleic acid detection cycle in the laboratory is short, and the sensitivity and specificity are comparable to those of the culture method. At present, nucleic acid detection reagent tubes are usually used for nucleic acid detection of samples. Separate, and then melt the separation layer by heating, so that the magnetic beads can carry nucleic acid through the separation layer for corresponding operations, but because nucleic acid is sensitive to temperature changes, in order to reduce the influence of temperature changes on nucleic acids, operators need to strictly control the heating area, so will increase the difficulty of operation

At the same time, because the existing detection tubes are susceptible to the interference of many factors (such as the interference of temperature on nucleic acid), there are large errors in the existing detection results

Moreover, the existing cleaning solution contains alcohols, which will not only inhibit the amplification reaction, but also be unfavorable for the dispersion of magnetic beads, and because alcohols are volatile, they are not conducive to product storage, thus Affect detection accuracy

Therefore, the existing technology has the problems of high operational difficulty, low detection accuracy and low detection efficiency.

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