Preparation method for cinnamaldehyde surface molecularly imprinted polymer

A technology of surface molecular imprinting and cinnamic acid, applied in material separation, analytical materials, ion exchange, etc., can solve the problems of cumbersome preparation methods, unstable column efficiency, uneven particle size, etc., and achieve simple and easy preparation process, molecular Strong identification ability and uniform grinding particle size

Active Publication Date: 2014-12-03
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have strong specificity, they have certain disadvantages. Because the recognition site is deeply buried, it is difficult to enter and elute template molecules, so the adsorption amount is small, and the preparation method is relatively cumbersome. The emergence of surface molecular imprinting overcomes the above problems. shortcoming
Although it has been reported in the literature that the molecularly imprinted polymers prepared have good selective adsorption for pefloxacin, the methods used are basically the traditional host polymerization and precipitation polymerization methods, and these methods exist as follows The disadvantages of the main polymerization method, the polymer prepared by it needs to be ground, this process will destroy many recognition sites, and many recognition sites are covered, the elution and adsorption of template molecules are very difficult, the specific surface area is small, and the particle size is small. Uneven diameters lead to unstable column efficiency and other phenomena

Method used

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  • Preparation method for cinnamaldehyde surface molecularly imprinted polymer
  • Preparation method for cinnamaldehyde surface molecularly imprinted polymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of cinnamic acid surface molecularly imprinted polymer

[0024] 1. Activation of silica gel

[0025] Dissolve 10g gelatin in 100ml0.25mol / L NaOH, shake for 30min, vacuum filter and wash until neutral, then dissolve it in 200ml8mol / LHCl, stir and reflux at 90°C for 12h, wash with ultrapure water until neutral, then vacuum dry, Obtain activated silica gel.

[0026] 2. Alkylated silica gel

[0027] Activated silica gel and silylating agent 3-methoxyaminopropylsilane were stirred and refluxed in toluene solution at 90°C for 12 hours, washed and vacuum-dried to obtain alkylated silica gel;

[0028] 3. Preparation of polymer

[0029] Take 1 g of alkylated silica gel, 1 mmol of cinnamic acid, 8 mol of methacrylic acid, 5 mol of ethylene glycol dimethacrylate, and 0.20 mmol of azobisisobutyronitrile, dissolve them in 50 ml of chloroform by volume, and mix them well in 60 ℃ water bath shaking thermal polymerization for 48h;

[0030] 4. Washing of templ...

Embodiment 2

[0032] Example 2 Application of Cinnamic Acid Surface Molecularly Imprinted Polymer

[0033] 1. Column packing and activation of solid phase extraction column

[0034] Take an empty polypropylene solid-phase extraction column with a capacity of 3ml, add a sieve plate of appropriate size, weigh 250mg of cinnamic acid molecularly imprinted solid-phase extraction materials (MIPs) and add it to it, and then add a sieve plate to the top of the packing, and use a sieve plate to Press the plate insertion tool tightly, and take 5ml of water-activated solid-phase extraction column;

[0035] 2. Sample pre-extraction

[0036] Cosmetic samples were ultrasonically extracted by adding methanol, and then filtered with a 0.22 μm microporous membrane.

[0037] 3. Sample loading, rinsing, elution and drying to volume

[0038]Add the above sample extract into the column, and control the solution passing through the column at a speed of no more than 0.5ml / min. After all the solution flows out,...

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Abstract

The invention relates to a preparation method for a cinnamaldehyde surface molecularly imprinted polymer. Cinnamic acid and a cinnamic acid polymer with specific recognition characteristic are prepared by adopting a molecular imprinting technology. The molecularly imprinted polymer takes alkylated silica as a carrier, analogue cinnamic acid of cinnamaldehyde as a template, 4-vinylpyridine as a functional monomer and polyethylene glycol dimethacrylate as a cross-linking agent. The obtained molecular imprinting polymer is synthesized into trichloromethane by adopting a thermal polymerization method. The obtained molecular imprinting polymer is used as filler of a solid phase extraction column for self preparing a solid-phase extraction column with the specificity. Meanwhile, the solid-phase extraction column and liquid chromatography are combined and applied to detection of cinnamaldehyde and analogue in an actual cosmetic sample. According to the preparation method disclosed by the invention, high-efficiency separation, enrichment and purification of essence cinnamaldehyde in the cosmetics are realized; the defects of a traditional solid-phase extraction column are overcome; and the obtained solid-phase extraction column can be repeatedly used and is low in cost and favorable in application prospect.

Description

technical field [0001] The invention relates to a preparation method of a polymer imprinted with cinnamaldehyde molecules on the surface of silica gel, belonging to the fields of cosmetic detection and drug analysis. Background technique [0002] Cinnamaldehyde, commonly known as cinnamaldehyde, occurs naturally in essential oils such as Sri Lankan cinnamon oil, cassia bark oil, patchouli oil, and hyacinth oil. Due to its unique properties, it has a wide range of applications, such as medicine, chemical industry, food, flavors and fragrances, and animal feed. Cinnamaldehyde is commonly used as a flavor in cosmetics. However, with the continuous increase of cosmetics and the expansion of the scope of use, there are more and more cosmetic allergies. Cinnamaldehyde is also one of the main allergens that cause cosmetic allergies, and more and more attracted the attention of consumers. [0003] The detection methods of cinnamaldehyde mainly include liquid chromatography, gas ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F222/14C08F226/06C08F2/44C08J9/28B01D15/08G01N30/06
Inventor 孙秀兰田秀梅蒋栋磊吴龙云张银志余雯静郑利钱和
Owner JIANGNAN UNIV
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